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Literature summary for 4.2.1.104 extracted from

  • Harano, Y.; Suzuki, I.; Maeda, S.; Kaneko, T.; Tabata, S.; Omata, T.
    Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942 (1997), J. Bacteriol., 179, 5744-5750.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.104

Activating Compound

Activating Compound Comment Organism Structure
bicarbonate
-
 Synechococcus sp.
bicarbonate
-
 Synechocystis sp.
NtcA transcription factor, global nitrogen regulator of cyanobacteria, required for expression Synechococcus sp.
NtcA transcription factor, global nitrogen regulator of cyanobacteria, required for expression Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene cynS, DNA and amino acid sequence determination and analysis, tightly clustered with 2 genes located upstream, which encode proteins similar to the subunits of nitrate-nitrite transporter, complementation of deficient Escherichia coli strain Synechococcus sp.
gene cynS, tightly clustered with 4 putative molybdenum cofactor biosynthesis genes located downstream Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
NH4+ negative regulation, represses enzyme expression Synechococcus sp.
NH4+ negative regulation, represses enzyme expression Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16362
-
 x * 16362, amino acid sequence calculation Synechococcus sp.

Organism

Organism UniProt Comment Textmining
Synechococcus sp.
-
 strain PCC 7942, gene cynS
-
Synechocystis sp.
-
 strain PCC 6803, gene cynS
-

Reaction

Reaction Comment Organism Reaction ID
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamae. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide Synechococcus sp.
a
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamate. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide Synechocystis sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.047
-
 complemented deficient Escherichia coli cells Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NCO- + HCO3-
-
 Synechococcus sp. NH4+ + CO2
-
 ?
NCO- + HCO3-
-
 Synechocystis sp. NH4+ + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 16362, amino acid sequence calculation Synechococcus sp.

Synonyms

Synonyms Comment Organism
cyanase
-
 Synechococcus sp.
cyanase
-
 Synechocystis sp.
EC 4.3.99.1 formerly Synechococcus sp.
EC 4.3.99.1 formerly Synechocystis sp.