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Information on EC 4.1.1.55 - 4,5-dihydroxyphthalate decarboxylase for references in articles please use BRENDA:EC4.1.1.55
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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
Decarboxylase, 4,5-dihydroxyphthalate, DHP decarboxylase,
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Decarboxylase, 4,5-dihydroxyphthalate
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DHP decarboxylase
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4,5-dihydroxyphthalate = 3,4-dihydroxybenzoate + CO2
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4,5-dihydroxyphthalate carboxy-lyase (3,4-dihydroxybenzoate-forming)
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
4-Hydroxyphthalate
3-Hydroxybenzoate
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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?
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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4,5-Dihydroxyphthalate
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induced by phthalate
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4,5-Dihydroxyphthalate
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induced by phthalate
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4-Hydroxyphthalate
3-Hydroxybenzoate
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4-Hydroxyphthalate
3-Hydroxybenzoate
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4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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?
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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?
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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?
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
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?
4,5-Dihydroxyphthalate
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induced by phthalate
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4,5-Dihydroxyphthalate
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induced by phthalate
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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4,5-Dihydroxyphthalate
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enzyme is involved in o-phthalate catabolism
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2,4,6-Trinitrobenzene sulfonate
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inhibition is partially prevented by 4,5-dihydroxyphthalate or 4-hydroxyphthalate, 1 mM
5,5'-dithiobis(2-nitrobenzoate)
anthranilate
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competitive
terephthalate
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competitive
5,5'-dithiobis(2-nitrobenzoate)
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5,5'-dithiobis(2-nitrobenzoate)
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PCMB
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0.0104 - 0.0105
4,5-Dihydroxyphthalate
1.25
4-Hydroxyphthalate
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0.0104
4,5-Dihydroxyphthalate
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0.0105
4,5-Dihydroxyphthalate
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brenda
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brenda
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brenda
no activity in Bacillus sp.
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brenda
no activity in Bacillus sp. FO
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
; strain NH1024, strain NH1021, strain NH1051, strain NH1027
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brenda
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brenda
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brenda
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38000
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4 * 38000, SDS-PAGE
66000
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6 * 66000, SDS-PAGE
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hexamer
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6 * 66000, SDS-PAGE
hexamer
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6 * 66000, SDS-PAGE
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tetramer
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4 * 38000, SDS-PAGE
tetramer
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4 * 38000, SDS-PAGE
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7.5
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27°C, 3.5 h, about 20% loss of activity
4714
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27
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pH 7.5, 3.5 h, about 20% loss of activity
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dialysis against distilled water or phosphate buffer, 0.067 M, pH 5.9-8.5, at 0-3°C, results in no appreciable loss of activity. Dialysis for 3-4 days causes about 70% loss of activity
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no inactivation by dialysis against 0.2 mM of either EDTA or o-phenanthroline in 0.05 M Tris-acetate, pH 7.5, containing 0.2 mM dithiothreitol, at 4°C, overnight
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4°C, 0.05 M Tris-acetate, pH 7.5, 0.2 mM dithiothreitol, 50% loss of activity after 4 days
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Pujar, B.G.; Ribbons, D.W.
Phthalate metabolism in Pseudomonas fluorescens PHK: purification and properties of 4,5-dihydroxyphthalate decarboxylase
Appl. Environ. Microbiol.
49
374-376
1985
Pseudomonas fluorescens, Pseudomonas fluorescens PHK
brenda
Taylor, B.F.; Ribbons, D.W.
Bacterial decarboxylation of o-phthalic acid
Appl. Environ. Microbiol.
46
1276-1281
1983
Comamonas testosteroni, no activity in Bacillus sp., no activity in Bacillus sp. FO
brenda
Nakazawa, T.; Hayashi, E.
Phthalate and 4-hydroxyphthalate metabolism in Pseudomonas testosteroni: purification and properties of 4,5-dihydroxyphthalate decarboxylase
Appl. Environ. Microbiol.
36
264-269
1978
Comamonas testosteroni, Comamonas testosteroni NH1000
brenda
Ribbons, D.W.; Evans, W.C.
Oxidative metabolism of phthalic acid soil Pseudomonads
Biochem. J.
76
310-318
1960
Pseudomonas sp.
brenda
Nakazawa, T.; Hayashi, E.
Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain
J. Bacteriol.
131
42-48
1977
Comamonas testosteroni, Comamonas testosteroni NH1000
brenda
Grifoll, M.; Selifonov, S.A.; Chapman, P.J.
Evidence for a novel pathway in the degradation of fluorene by Pseudomonas sp. strain F274
Appl. Environ. Microbiol.
60
2438-2449
1994
Pseudomonas sp., Pseudomonas sp. F274
brenda
Iwaki, H.; Nishimura, A.; Hasegawa, Y.
Isolation and characterization of marine bacteria capable of utilizing phthalate
World J. Microbiol. Biotechnol.
28
1321-1325
2012
Burkholderia cepacia, Burkholderia cepacia DBO1 / ATCC 29424, Pseudomonas putida, Pseudomonas putida NMH102-2
brenda
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4.1.1.55 4,5-dihydroxyphthalate decarboxylase
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