Information on EC 4.1.1.55 - 4,5-dihydroxyphthalate decarboxylase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
4.1.1.55
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RECOMMENDED NAME
GeneOntology No.
4,5-dihydroxyphthalate decarboxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4,5-dihydroxyphthalate = 3,4-dihydroxybenzoate + CO2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Polycyclic aromatic hydrocarbon degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
4,5-dihydroxyphthalate carboxy-lyase (3,4-dihydroxybenzoate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37290-48-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Burkholderia cepacia DBO1 / ATCC 29424
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Manually annotated by BRENDA team
no activity in Bacillus sp.
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Manually annotated by BRENDA team
no activity in Bacillus sp. FO
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
show the reaction diagram
4,5-Dihydroxyphthalate
?
show the reaction diagram
4-Hydroxyphthalate
3-Hydroxybenzoate
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4,5-Dihydroxyphthalate
3,4-Dihydroxybenzoate + CO2
show the reaction diagram
4,5-Dihydroxyphthalate
?
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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2,4,6-Trinitrobenzene sulfonate
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inhibition is partially prevented by 4,5-dihydroxyphthalate or 4-hydroxyphthalate, 1 mM
3-hydroxybenzoate
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3-methylcatechol
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weak
4-hydroxybenzoate
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5,5'-dithiobis(2-nitrobenzoate)
anthranilate
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competitive
ATP
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slight
CoA
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slight
gentisate
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weak
protocatechuate
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salicylate
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terephthalate
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competitive
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0104 - 0.0105
4,5-Dihydroxyphthalate
1.25
4-Hydroxyphthalate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.7
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
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4 * 38000, SDS-PAGE
66000
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6 * 66000, SDS-PAGE
150000
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gel filtration
420000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
tetramer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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27°C, 3.5 h, about 20% loss of activity
4714
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
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pH 7.5, 3.5 h, about 20% loss of activity
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis against distilled water or phosphate buffer, 0.067 M, pH 5.9-8.5, at 0-3°C, results in no appreciable loss of activity. Dialysis for 3-4 days causes about 70% loss of activity
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no inactivation by dialysis against 0.2 mM of either EDTA or o-phenanthroline in 0.05 M Tris-acetate, pH 7.5, containing 0.2 mM dithiothreitol, at 4°C, overnight
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 0.05 M Tris-acetate, pH 7.5, 0.2 mM dithiothreitol, 50% loss of activity after 4 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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Show AA Sequence (145 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 4.1.1.55)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)