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Literature summary for 4.1.1.55 extracted from

  • Nakazawa, T.; Hayashi, E.
    Phthalate and 4-hydroxyphthalate metabolism in Pseudomonas testosteroni: purification and properties of 4,5-dihydroxyphthalate decarboxylase (1978), Appl. Environ. Microbiol., 36, 264-269.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
no inactivation by dialysis against 0.2 mM of either EDTA or o-phenanthroline in 0.05 M Tris-acetate, pH 7.5, containing 0.2 mM dithiothreitol, at 4°C, overnight Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzene sulfonate inhibition is partially prevented by 4,5-dihydroxyphthalate or 4-hydroxyphthalate, 1 mM Comamonas testosteroni
3-hydroxybenzoate
-
Comamonas testosteroni
3-methylcatechol weak Comamonas testosteroni
4-hydroxybenzoate
-
Comamonas testosteroni
AgCl
-
Comamonas testosteroni
anthranilate competitive Comamonas testosteroni
benzoate
-
Comamonas testosteroni
gentisate weak Comamonas testosteroni
HgCl2
-
Comamonas testosteroni
PCMB
-
Comamonas testosteroni
Phthalate
-
Comamonas testosteroni
protocatechuate
-
Comamonas testosteroni
salicylate
-
Comamonas testosteroni
terephthalate competitive Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
4-Hydroxyphthalate
-
Comamonas testosteroni
0.0105
-
4,5-Dihydroxyphthalate
-
Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Comamonas testosteroni
38000
-
4 * 38000, SDS-PAGE Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-
Comamonas testosteroni NH1000
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.9
-
-
Comamonas testosteroni

Storage Stability

Storage Stability Organism
4°C, 0.05 M Tris-acetate, pH 7.5, 0.2 mM dithiothreitol, 50% loss of activity after 4 days Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,5-Dihydroxyphthalate
-
Comamonas testosteroni 3,4-Dihydroxybenzoate + CO2
-
?
4,5-Dihydroxyphthalate
-
Comamonas testosteroni NH1000 3,4-Dihydroxybenzoate + CO2
-
?
4-Hydroxyphthalate
-
Comamonas testosteroni 3-Hydroxybenzoate
-
?
4-Hydroxyphthalate
-
Comamonas testosteroni NH1000 3-Hydroxybenzoate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38000, SDS-PAGE Comamonas testosteroni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
27
-
pH 7.5, 3.5 h, about 20% loss of activity Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Comamonas testosteroni

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
27°C, 3.5 h, about 20% loss of activity Comamonas testosteroni