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Information on EC 4.1.1.31 - phosphoenolpyruvate carboxylase and Organism(s) Arabidopsis thaliana and UniProt Accession Q8GVE8

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     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.31 phosphoenolpyruvate carboxylase
IUBMB Comments
This enzyme replenishes oxaloacetate in the tricarboxylic acid cycle when operating in the reverse direction. The reaction proceeds in two steps: formation of carboxyphosphate and the enolate form of pyruvate, followed by carboxylation of the enolate and release of phosphate.
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Select one or more organisms in this record:
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q8GVE8
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The taxonomic range for the selected organisms is: Arabidopsis thaliana
Synonyms
archaeal-type phosphoenolpyruvate carboxylase, AT1G53310, atPEPC, Atppc1, Atppc2, Atppc3, Atppc4, bacterial-type PEPC, bacterial-type phosphoenolpyruvate carboxylase, BPTC, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AT1G53310
282892
gene name
Atppc1
282892
-
Atppc2
282893
-
Atppc3
282894
-
Atppc4
282895
-
bacterial-type phosphoenolpyruvate carboxylase
302
-
Carboxylase, phosphopyruvate (phosphate)
-
-
-
-
CP21
-
-
-
-
CP28
-
-
-
-
CP46
-
-
-
-
PEP carboxylase
PEPC1
302
isozyme
PEPCase
-
-
-
-
phosphoenolpyruvate carboxykinase
302
-
Phosphoenolpyruvate carboxylase
Phosphoenolpyruvic carboxylase
-
-
-
-
plant-type phosphoenolpyruvate carboxylase
302
-
PPC1
302
isozyme
PPCK1
302
isozyme
PPCK2
302
isozyme
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylation
-
-
-
-
decarboxylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphate:oxaloacetate carboxy-lyase (adding phosphate; phosphoenolpyruvate-forming)
This enzyme replenishes oxaloacetate in the tricarboxylic acid cycle when operating in the reverse direction. The reaction proceeds in two steps: formation of carboxyphosphate and the enolate form of pyruvate, followed by carboxylation of the enolate and release of phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9067-77-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + CO2
phosphate + oxaloacetate
show the reaction diagram
-
-
-
?
phosphate + oxaloacetate
phosphoenolpyruvate + CO2
show the reaction diagram
-
-
-
-
?
phosphoenolpyruvate + CO2
phosphate + oxaloacetate
show the reaction diagram
phosphoenolpyruvate + HCO3-
phosphate + oxaloacetate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
absolute dependence
Mn2+
-
absolute dependence
additional information
-
Cd2 + toxicity leads to PEPC up-regulation, iron deficiency also up-regulates PEPC activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-aspartate
-
-
additional information
-
not inhibited by phosphatidic acid, phosphatidylinositol, phosphatidylinositol 4-phosphate, lyso-phosphatidic acid, phosphatidylserine, phosphatidylcholine, and phosphatidylethanolamine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucose 6-phosphate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.054 - 0.34
phosphoenolpyruvate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.52 - 1.14
L-aspartate
0.2 - 0.68
L-malate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.034
-
clarified extract, at pH 8.5
22.3
-
after 660fold purification, at pH 8.5
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcript detected in
Manually annotated by BRENDA team
transcript detected in
Manually annotated by BRENDA team
transcript detected
Manually annotated by BRENDA team
-
BTPC shows limited expression during pollen development
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
BTPC localizes in vegetative cell cytoplasm
Manually annotated by BRENDA team
-
the enzyme partially localizes to membranes
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
CAPP4_ARATH
1032
0
116586
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
116600
deduced from cDNA
107000
-
4 * 107000, native enzyme, gel filtration
109700
deduced from cDNA
110200
deduced from cDNA
110300
deduced from cDNA
430000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
-
4 * 107000, native enzyme, gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.5
-
PEPC activity at pH 7.0 is approximately 50% of that occurring at pH 8.5
701501
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, butyl-Sepharose column chromatography, Fractogel EMD DEAE-650 colum chromatography, Mono Q column chromatography, and Superdex-200 gel filtration
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli with an N-terminal His-tag
expressed in Escherichia coli with an N-terminal His-tag
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
BTPC expression initiates after the last mitosis before pollen germination
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sanchez, R.; Flores, A.; Cejudo, F.J.
Arabidopsis phosphoenolpyruvate carboxylase genes encode immunologically unrelated polypeptides and are differentially expressed in response to drought and salt stress
Planta
223
901-909
2006
Arabidopsis thaliana (Q5GM68), Arabidopsis thaliana (Q84VW9), Arabidopsis thaliana (Q8GVE8), Arabidopsis thaliana (Q9MAH0)
Manually annotated by BRENDA team
Dharmarajan, L.; Kraszewski, J.L.; Mukhopadhyay, B.; Dunten, P.W.
Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase
Acta Crystallogr. Sect. F
65
1193-1196
2009
Arabidopsis thaliana
Manually annotated by BRENDA team
Meimoun, P.; Gousset-Dupont, A.; Lebouteiller, B.; Ambard-Bretteville, F.; Besin, E.; Lelarge, C.; Mauve, C.; Hodges, M.; Vidal, J.
The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: molecular and physiological characterization of PEPC kinase mutants
FEBS Lett.
583
1649-1652
2009
Arabidopsis thaliana
Manually annotated by BRENDA team
Monreal, J.; McLoughlin, F.; Echevarría, C.; García-Mauriño, S.; Testerink, C.
Phosphoenolpyruvate carboxylase from C4 leaves is selectively targeted for inhibition by anionic phospholipids
Plant Physiol.
152
634-638
2010
Arabidopsis thaliana, Sorghum bicolor, Zea mays
Manually annotated by BRENDA team
O'Leary, B.; Park, J.; Plaxton, W.C.
The remarkable diversity of plant PEPC (phosphoenolpyruvate carboxylase): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs
Biochem. J.
436
15-34
2011
Arabidopsis thaliana, Beta vulgaris, Brassica napus, Chlamydomonas reinhardtii, Citrus sinensis, Glycine max, Helianthus annuus, Hordeum vulgare, Lotus japonicus, Lupinus albus, Musa cavendishii, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum
Manually annotated by BRENDA team
Igawa, T.; Fujiwara, M.; Tanaka, I.; Fukao, Y.; Yanagawa, Y.
Characterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants
BMC Plant Biol.
10
200
2010
Arabidopsis thaliana, Lilium longiflorum
Manually annotated by BRENDA team
Shi, J.; Yi, K.; Liu, Y.; Xie, L.; Zhou, Z.; Chen, Y.; Hu, Z.; Zheng, T.; Liu, R.; Chen, Y.; Chen, J.
Phosphoenolpyruvate carboxylase in Arabidopsis leaves plays a crucial role in carbon and nitrogen metabolism
Plant Physiol.
167
671-681
2015
Arabidopsis thaliana (Q5GM68), Arabidopsis thaliana (Q9MAH0)
Manually annotated by BRENDA team
Feria, A.B.; Bosch, N.; Sanchez, A.; Nieto-Ingelmo, A.I.; de la Osa, C.; Echevarria, C.; Garcia-Maurino, S.; Monreal, J.A.
Phosphoenolpyruvate carboxylase (PEPC) and PEPC-kinase (PEPC-k) isoenzymes in Arabidopsis thaliana role in control and abiotic stress conditions
Planta
244
901-913
2016
Arabidopsis thaliana, Arabidopsis thaliana (Q84VW9)
Manually annotated by BRENDA team
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