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Information on EC 3.8.1.5 - haloalkane dehalogenase and Organism(s) Mycobacterium avium and UniProt Accession Q93K00

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EC Tree
     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.5 haloalkane dehalogenase
IUBMB Comments
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
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This record set is specific for:
Mycobacterium avium
UNIPROT: Q93K00
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The taxonomic range for the selected organisms is: Mycobacterium avium
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
haloalkane dehalogenase, haloalkane dehalogenase linb, dhaa31, hld-i, rv2579, ehld-c, linbut, ylehd, 1-chlorohexane halidohydrolase, linbmi, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-chlorohexane halidohydrolase
-
-
-
-
1-haloalkane dehalogenase
-
-
-
-
haloalkane dehalogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-haloalkane + H2O = a primary alcohol + halide
show the reaction diagram
catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-halide
C-halide hydrolysis
-
SYSTEMATIC NAME
IUBMB Comments
1-haloalkane halidohydrolase
Acts on a wide range of 1-haloalkanes, haloalcohols, haloalkenes and some haloaromatic compounds.
CAS REGISTRY NUMBER
COMMENTARY hide
95990-29-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dichloroethane + H2O
?
show the reaction diagram
-
-
-
?
1-bromobutane + H2O
1-butanol + bromide
show the reaction diagram
-
-
-
?
1-bromobutane + H2O
n-butanol + bromide
show the reaction diagram
-
-
-
?
1-haloalkane + H2O
a primary alcohol + halide
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-haloalkane + H2O
a primary alcohol + halide
show the reaction diagram
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00066
1,2-dichloroethane
0.6
1-bromobutane
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
activity assay with 1,2-dichloroethane
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene dhmA
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the alpha/beta-hydrolase superfamily, subgroup I of HLDs
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DHMA_MYCAV
301
0
33842
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
determined by SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D123A
D250A
H279A
W124L
W164L
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the cell free extract of Rhodococcus erythropolis IAM1399 cells is purified on a BD TALON metal affinity resin
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
phylogenetic analysis and tree
the coding sequence is amplified in the pAL-781-dhmA vector, the vector pUC18-dhmAHis is propagated in Escherichia coli JM110, the dhmAHis gene is ligated into the vector pK4RP for expression in Rhodococcus erythropolis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pavlova, M.; Klva?a, M.; Jesenska, A.; Prokop, Z.; Kone?na, H.; Sato, T.; Tsuda, M.; Nagata, Y.; Damborsky, J.
The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: Reaction mechanism and molecular evolution
J. Struct. Biol.
157
384-392
2006
Mycobacterium avium, Mycobacterium avium (Q73Y99), Mycobacterium avium N85
Manually annotated by BRENDA team
Chovancova, E.; Kosinski, J.; Bujnicki, J.M.; Damborsky, J.
Phylogenetic analysis of haloalkane dehalogenases
Proteins
67
305-316
2007
Mycobacterium tuberculosis, Mycobacterium avium, Mycolicibacterium smegmatis, Sphingobium indicum (A8CFB7), Rhodococcus sp. (P0A3G3), Xanthobacter autotrophicus (P22643), Bradyrhizobium japonicum (P59337), Rhodopirellula baltica (Q7UF99), Agrobacterium tumefaciens (Q8U671), Mesorhizobium loti (Q98C03)
Manually annotated by BRENDA team
Nagata, Y.; Ohtsubo, Y.; Tsuda, M.
Properties and biotechnological applications of natural and engineered haloalkane dehalogenases
Appl. Microbiol. Biotechnol.
99
9865-9881
2015
Agrobacterium tumefaciens (E2RV69), Agrobacterium tumefaciens C58 / ATCC 33970 (E2RV69), Alcanivorax dieselolei, Alcanivorax dieselolei B-5, Arthrobacter sp., Bradyrhizobium elkanii (E2RV62), Bradyrhizobium elkanii USDA94 (E2RV62), Bradyrhizobium japonicum (P59337), Bradyrhizobium japonicum USDA 110 (P59337), Corynebacterium sp., Marinobacter sp. (A3JB27), Mesorhizobium loti (Q98C03), Mesorhizobium loti MAFF303099 (Q98C03), Mycobacterium avium (Q93K00), Mycobacterium avium N85 (Q93K00), Mycobacterium sp. (Q9ZER0), Mycobacterium sp. GP1 (Q9ZER0), Mycobacterium tuberculosis (P9WMR9), Mycobacterium tuberculosis H37Rv (P9WMR9), Mycobacterium tuberculosis variant bovis (A4Q9R7), Mycobacterium tuberculosis variant bovis (Q6EUU8), Mycobacterium tuberculosis variant bovis (Q9XB14), Mycobacterium tuberculosis variant bovis 5033/66 (A4Q9R7), Mycobacterium tuberculosis variant bovis 5033/66 (Q6EUU8), Mycobacterium tuberculosis variant bovis 5033/66 (Q9XB14), Plesiocystis pacifica (A6G7B1), Psychrobacter cryohalolentis (Q1QBB9), Psychrobacter cryohalolentis K5 (Q1QBB9), Rhodobacteraceae bacterium UDC319 (A0A023I2Y1), Rhodococcus rhodochrous (P0A3G2), Rhodococcus rhodochrous NCIMB 13064 (P0A3G2), Rhodococcus sp., Rhodopirellula baltica (G3XCP3), Rhodopirellula baltica SH1 (G3XCP3), Sphingobium indicum, Sphingobium indicum (P51698), Sphingobium indicum B90A, Sphingobium indicum UT26 (P51698), Sphingobium sp. (A4PEU6), Sphingobium sp. MI1205 (A4PEU6), Sphingomonas sp., Sphingomonas sp. BHC-A, Strongylocentrotus purpuratus, Xanthobacter autotrophicus (P22643), Xanthobacter autotrophicus GJ10 (P22643)
Manually annotated by BRENDA team