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EC Tree
IUBMB Comments This enzyme hydrolyses the diphosphate bond, leaving a phospho group where a diphospho group had been. It can also act on bis(adenosine) diphosphate.
The taxonomic range for the selected organisms is: Homo sapiens The enzyme appears in selected viruses and cellular organisms
Synonyms
diphosphoinositol polyphosphate phosphohydrolase, dipp2, dipp3, mudipp1, diphosphoinositol-polyphosphate phosphohydrolase, aps protein, diphosphoinositol phosphohydrolase, diphosphoinositol phosphate phosphohydrolase,
more
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diphosphoinositol phosphohydrolase
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diphosphoinositol polyphosphate phosphatase
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diphosphoinositol polyphosphate phosphohydrolase
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diphosphoinositol-polyphosphate phosphohydrolase
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phosphatase, diphosphoinositol polyphosphate
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poly-P endopolyphosphatase
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DIPP3
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isoform
DIPP3
isoenzyme hDIPP3alpha
DIPP3
isoenzyme hDIPP3beta
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hydrolysis of phosphoric ester
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diphospho-myo-inositol-polyphosphate diphosphohydrolase
This enzyme hydrolyses the diphosphate bond, leaving a phospho group where a diphospho group had been. It can also act on bis(adenosine) diphosphate.
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diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
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-
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?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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reaction of EC 3.6.1.17
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?
bisdiphosphoinositol tetrakisphosphate + H2O
? + phosphate
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?
bisdiphosphoinositol tetrakisphosphate + H2O
diphosphoinositol pentakisphosphate + phosphate
dATP + H2O
dADP + phosphate
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?
diadenosine 5',5''-P1,P6-hexaphosphate + H2O
?
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?
diphosphoinositol pentakisphosphate + H2O
inositol hexakisphosphate + phosphate
diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
additional information
?
-
bisdiphosphoinositol tetrakisphosphate + H2O
diphosphoinositol pentakisphosphate + phosphate
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?
bisdiphosphoinositol tetrakisphosphate + H2O
diphosphoinositol pentakisphosphate + phosphate
specificity constant of isoenzyme hDIPP3beta is 2.7fold higher than value for isoenzyme hDIPP3alpha
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?
diphosphoinositol pentakisphosphate + H2O
inositol hexakisphosphate + phosphate
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?
diphosphoinositol pentakisphosphate + H2O
inositol hexakisphosphate + phosphate
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?
diphosphoinositol pentakisphosphate + H2O
inositol hexakisphosphate + phosphate
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the diphosphate group that is attached to the 5-carbon is hydrolyzed, no release of diphosphate
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?
diphosphoinositol pentakisphosphate + H2O
inositol hexakisphosphate + phosphate
isoenzyme hDIPP3beta is 2.3fold more active than isoenzyme hDIPP3alpha
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?
diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
no activity with dGTP
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?
diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
vesicle trafficking, stress responses, DNA repair, apoptosis
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?
additional information
?
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enzyme degrades Ap6A and Ap5A in preference to other diadenosine polyphosphates
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?
additional information
?
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the MuT domain of the enzyme participates in the hydrolysis of dATP, diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
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?
additional information
?
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DIPP1, DIPP2 and DIPP3 are also capable of degrading polyphosphates
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?
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diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
vesicle trafficking, stress responses, DNA repair, apoptosis
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-
?
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inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,4,5,6-pentaphosphate
competitive
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
competitive
inositol 4-butyl-1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
competitive
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0.0000042 - 0.0000048
diphosphoinositol pentakisphosphate
additional information
additional information
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Km-values for hydrolysis of Ap6A and Ap5A
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0.0000042
diphosphoinositol pentakisphosphate
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0.0000042
diphosphoinositol pentakisphosphate
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isoenzyme hDIPP1 and hDIPP2alpha
0.0000048
diphosphoinositol pentakisphosphate
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isoenzyme hDIPP2beta
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0.03 - 0.2
diphosphoinositol pentakisphosphate
additional information
additional information
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turnover-numbers for hydrolysis of Ap6A and Ap5A
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0.03
diphosphoinositol pentakisphosphate
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0.03
diphosphoinositol pentakisphosphate
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isoenzyme hDIPP2beta
0.15
diphosphoinositol pentakisphosphate
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0.15
diphosphoinositol pentakisphosphate
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isoenzyme hDIPP2alpha
0.2
diphosphoinositol pentakisphosphate
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-
0.2
diphosphoinositol pentakisphosphate
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isoenzyme hDIPP1
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0.00011
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,4,5,6-pentaphosphate
pH not specified in the publication, temperature not specified in the publication
0.00027
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
pH not specified in the publication, temperature not specified in the publication
0.000023
inositol 4-butyl-1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
pH not specified in the publication, temperature not specified in the publication
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7 - 7.5
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hydrolysis of diphosphoinositol pentakisphosphate
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6.5 - 8
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pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate
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SwissProt
brenda
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brenda
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brenda
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NUDT3_HUMAN
172
0
19471
Swiss-Prot
other Location (Reliability: 2 )
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19500
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x * 19500, calculation from nucleotide sequence
20434
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x * 20434, calculation from nucleotide sequence
25000
recombinant enzyme, SDS-PAGE
25000
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x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
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?
-
x * 19500, calculation from nucleotide sequence
?
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x * 25000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
?
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x * 20434, calculation from nucleotide sequence
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E66Q
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no significant activity towards diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate, activity with diadenosine 5',5'''-P1,P6-hexaphosphate is 2% of the activity of the wild-type enzyme
F84Y
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hydrolysis of bisdiphosphoinositol tetrakisphosphate at 63% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 75% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
G50A
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hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
G52A
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hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
G72A
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catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
G75A
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catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
G78A
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catalytic activity and CD spectrum is not significantly different from that of the wild.type enzyme
G82A
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mutation promotes some unraveling of the normal secondary structure
H91L
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hydrolysis of bisdiphosphoinositol tetrakisphosphate at 4% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 26% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
P89R
codes for isoenzyme hDIPP3beta
E70Q
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E70Q
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inactive against dATP,diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate
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recombinant enzyme, by TALON metal affinity chromatography
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expression in Escherichia coli
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isoenzyme hDIPP2alpha and hDIPP2beta
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Safrany, S.T.; Caffrey, J.J.; Yang, X.; Bembenek, M.E.; Moyer, M.B.; Burkhart, W.A.; Shears, S.B.
A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase
EMBO J.
17
6599-6607
1998
Homo sapiens, Rattus norvegicus
brenda
Safrany, S.T.; Ingram, S.W.; Cartwright, J.L.; Falck, J.R.; McLennan, A.G.; Barnes, L.D.; Shears, S.B.
The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein
J. Biol. Chem.
274
21735-21740
1999
Homo sapiens
brenda
Yang, X.; Safrany, S.T.; Shears, S.B.
Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates
J. Biol. Chem.
274
35434-35440
1999
Homo sapiens
brenda
Caffrey, J.J.; Safrany, S.T.; Yang, X.; Shears, S.B.
Discovery of molecular and catalytic diversity among human diphosphoinositol-polyphosphate phosphohydrolases. An expanding NUDT family
J. Biol. Chem.
275
12730-12736
2000
Homo sapiens
brenda
Hidaka, K.; Caffrey, J.J.; Hua, L.; Zhang, T.; Falck, J.R.; Nickel, G.C.; Carrel, L.; Barnes, L.D.; Shears, S.B.
An adjacent pair of human NUDT genes on chromosome X are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase
J. Biol. Chem.
277
32730-32738
2002
Homo sapiens (P07202), Homo sapiens (Q96G61), Homo sapiens
brenda
Lonetti, A.; Szijgyarto, Z.; Bosch, D.; Loss, O.; Azevedo, C.; Saiardi, A.
Identification of an evolutionarily conserved family of inorganic polyphosphate endopolyphosphatases
J. Biol. Chem.
286
31966-31974
2011
Homo sapiens
brenda
Wu, M.; Chong, L.S.; Capolicchio, S.; Jessen, H.J.; Resnick, A.C.; Fiedler, D.
Elucidating diphosphoinositol polyphosphate function with nonhydrolyzable analogues
Angew. Chem. Int. Ed. Engl.
53
7192-7197
2014
Homo sapiens (O95989)
brenda