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Information on EC 3.6.1.40 - guanosine-5'-triphosphate,3'-diphosphate phosphatase and Organism(s) Escherichia coli and UniProt Accession P25552
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3.6.1.40 guanosine-5'-triphosphate,3'-diphosphate phosphataseIUBMB Comments
Also hydrolyses other guanosine 5'-triphosphate derivatives with at least one unsubstituted phosphate group on the 3'-position, but not GTP, ATP or adenosine 5'-triphosphate 3'-diphosphate.
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
Synonyms
pppgpp-5'-phosphohydrolase, exopolyphosphatase/guanosine pentaphosphate phosphohydrolase, guanosine pentaphosphatase, guanosine pentaphosphate phosphohydrolase, guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, pppgpp phosphohydrolase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
guanosine 5'-triphosphate-3'-diphosphate 5'-phosphohydrolase
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guanosine pentaphosphatase
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guanosine pentaphosphate phosphatase
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guanosine pentaphosphate phosphohydrolase
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guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
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phosphatase, guanosine 5'-triphosphate 3'-diphosphate 5'-
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pppGpp 5'-phosphohydrolase
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pppGpp-5'-phosphohydrolase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric acid anhydride
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SYSTEMATIC NAME
IUBMB Comments
guanosine-5'-triphosphate,3'-diphosphate 5'-phosphohydrolase
Also hydrolyses other guanosine 5'-triphosphate derivatives with at least one unsubstituted phosphate group on the 3'-position, but not GTP, ATP or adenosine 5'-triphosphate 3'-diphosphate.
CAS REGISTRY NUMBER
COMMENTARY
85130-44-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
guanosine 5'-triphosphate 3'-diphosphate + H2O
guanosine 3',5'-bis(diphosphate) + phosphate
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?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
guanosine 5'-triphosphate,3'-monophosphate + H2O
guanosine 5'-diphosphate,3'-monophosphate + phosphate
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?
pppGpNp + H2O
ppGpNp + phosphate
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cleavage of 5'-phosphate ends of RNA
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?
pppGpNp-RNA + H2O
ppGpNp-RNA + phosphate
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cleavage of 5'-phosphate ends of RNA, poor substrate
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?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
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?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
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?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
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?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
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involved in nutritional stress response via ppGpp
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?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
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involved in nutritional stress response via ppGpp
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?
additional information
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not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential
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additional information
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enzyme also liberates phosphate by processive hydrolysis of the phosphoanhydride bonds of polyphosphate chains (1000 residues)
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
guanosine 5'-triphosphate 3'-diphosphate + H2O
guanosine 3',5'-bis(diphosphate) + phosphate
-
-
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
-
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
involved in nutritional stress response via ppGpp
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
involved in nutritional stress response via ppGpp
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, apoenzyme, using 10% (w/v) PEG 3350, 0.1 M HEPES (pH 7.5), and 0.2 M magnesium chloride
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Keasling, J.D.; Bertsch, L.; Kornberg, A.
Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase
Proc. Natl. Acad. Sci. USA
90
7029-7033
1993
Escherichia coli, Escherichia coli CA10
Hara, A.; Sy, J.
Guanosine 5'-triphosphate, 3'-diphosphate 5'-phosphohydrolase. Purification and substrate specificity
J. Biol. Chem.
258
1678-1683
1983
Escherichia coli, Escherichia coli JF1599
Reizer, J.; Reizer, A.; Saier, M.H.
Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily
Trends Biochem. Sci.
18
247-248
1993
Escherichia coli
Song, H.; Dharmasena, M.N.; Wang, C.; Shaw, G.X.; Cherry, S.; Tropea, J.E.; Jin, D.J.; Ji, X.
Structure and activity of PPX/GppA homologs from Escherichia coli and Helicobacter pylori
FEBS J.
287
1865-1885
2019
Escherichia coli (P25552), Helicobacter pylori (C7BYL8), Helicobacter pylori B38 (C7BYL8)
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