EC Number |
---|
3.6.1.40 | PPX/GPPA Y19N mutant enzyme in complex with guanosine tetraphosphate, hanging-drop vapor diffusion method by mixing 0.002 ml of protein and 0.002 ml of reservoir solution containing 200 mM sodium acetate, pH 6.4, 100 mM 4-morpholineethanesulfonic acid, pH 5.7, and 50% methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 2.7 A resolution, modelling |
3.6.1.40 | sitting drop vapor diffusion method, apoenzyme, using 10% (w/v) PEG 3350, 0.1 M HEPES (pH 7.5), and 0.2 M magnesium chloride |
3.6.1.40 | sitting drop vapor diffusion method. Apoenzyme, using 20% (w/v) PEG 3350, 0.2 M ammonium sulfate, and 15 mM malic acid. Enzyme in complex with 5'-guanosine-diphosphate-monothiophosphate or phosphoaminophosphonic acid guanylate ester, using 20% (w/v) PEG 3350 and 0.2 M potassium chloride. Enzyme in complex with guanosine tetraphosphate, using 20% (w/v) PEG 3350, 0.2 M ammonium tartrate, and 15 mM malic acid |
3.6.1.40 | two-domain structure, two crystal forms |
3.6.1.40 | X-ray diffraction structure determination and analysis of two crystal forms at 1.53 A and 2.15 A resolution, two domains |