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Information on EC 3.6.1.27 - undecaprenyl-diphosphate phosphatase and Organism(s) Bacillus subtilis and UniProt Accession P94571

for references in articles please use BRENDA:EC3.6.1.27
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IUBMB Comments
Isolated from the bacteria Micrococcus lysodeikticus , Escherichia coli [2,3,5,6] and Bacillus subtilis . The product of the reaction, ditrans,octacis-undecaprenyl phosphate, is essential for cell wall polysaccharide biosynthesis in these strains.
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This record set is specific for:
Bacillus subtilis
UNIPROT: P94571
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
undecaprenyl pyrophosphate phosphatase, upp phosphatase, smu.244, c55-pp phosphatase, undecaprenyl diphosphate phosphatase, c55-pp pyrophosphatase, hp0851, undecaprenyl pyrophosphatase, upp-pase, undecaprenyl-diphosphate phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Und-pp phosphatase
-
undecaprenyl pyrophosphate phosphatase
-
C55-isoprenyl diphosphatase
-
-
-
-
C55-isoprenyl pyrophosphatase
-
-
-
-
Dolicholpyrophosphatase
-
-
-
-
isoprenyl pyrophosphatase
-
-
-
-
undecaprenyl pyrophosphate phosphatase
-
-
UPP phosphatase
-
-
UPP-Pase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ditrans,octacis-undecaprenyl-diphosphate phosphohydrolase
Isolated from the bacteria Micrococcus lysodeikticus [1], Escherichia coli [2,3,5,6] and Bacillus subtilis [4]. The product of the reaction, ditrans,octacis-undecaprenyl phosphate, is essential for cell wall polysaccharide biosynthesis in these strains.
CAS REGISTRY NUMBER
COMMENTARY hide
9077-80-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
undecaprenyl diphosphate + H2O
undecaprenyl phosphate + phosphate
show the reaction diagram
-
-
-
?
undecaprenyl diphosphate + H2O
undecaprenyl phosphate + phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
undecaprenyl diphosphate + H2O
undecaprenyl phosphate + phosphate
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bacitracin
-
-
additional information
-
no inhibitory effect by reserpine
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.198
-
non-purified
0.23
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after first step of n-dodecyl-beta-D-maltoside extraction
0.73
-
purified His-tagged BcrC
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
inner cytoplasmic membrane
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme confers resistance to bacitracin
malfunction
physiological function
-
the enzyme BcrC is more relevant during vegetative growth while the enzyme UppP is crucial for normal sporulation. Together, enzyme forms bcrC and uppP encode the essential undecaprenyl diphosphatase function of Bacillus subtilis
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22000
-
SDS-PAGE, His-tagged BcrC
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bernard, R.; El Ghachi, M.; Mengin-Lecreulx, D.; Chippaux, M.; Denizot, F.
BcrC from Bacillus subtilis acts as an undecaprenyl pyrophosphate phosphatase in bacitracin resistance
J. Biol. Chem.
280
28852-28857
2005
Bacillus subtilis
Manually annotated by BRENDA team
Coker, O.; Palittapongarnpim, P.
Current understanding of de novo synthesis of bacterial lipid carrier (undecaprenyl phosphate): More enzymes to be discovered
Afr. J. Microbiol. Res.
5
2555-2565
2011
Escherichia coli (P60932), Bacillus subtilis (P94571)
-
Manually annotated by BRENDA team
Radeck, J.; Lautenschlaeger, N.; Mascher, T.
The essential UPP phosphatase pair BcrC and UppP connects cell wall homeostasis during growth and sporulation with cell envelope stress response in Bacillus subtilis
Front. Microbiol.
8
2403
2017
Bacillus subtilis
Manually annotated by BRENDA team
Zhao, H.; Sun, Y.; Peters, J.M.; Gross, C.A.; Garner, E.C.; Helmann, J.D.
Depletion of undecaprenyl pyrophosphate phosphatases disrupts cell envelope biogenesis in Bacillus subtilis
J. Bacteriol.
198
2925-2935
2016
Bacillus subtilis
Manually annotated by BRENDA team