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Information on EC 3.5.2.18 - enamidase for references in articles please use BRENDA:EC3.5.2.18Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Eubacterium barkeri
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6-oxo-1,4,5,6-tetrahydronicotinate + 2 H2O = 2-formylglutarate + NH3
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hydrolysis of C-N bond
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nicotinate degradation III
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Nicotinate and nicotinamide metabolism
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Microbial metabolism in diverse environments
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6-oxo-1,4,5,6-tetrahydronicotinate amidohydrolase
Contains iron and Zn2+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 1.1.1.291 (2-hydroxymethylglutarate dehydrogenase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Delta-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase).
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PDB: 2vun
UniProt
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1,4,5,6-tetrahydro-6-oxonicotinate + H2O
2-(enamine)glutarate + NH3
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2-(enamine)glutarate + H2O
(S)-2-formylglutarate + NH3
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6-oxo-1,4,5,6-tetrahydronicotinate + H2O
2-formylglutarate + NH3
6-oxo-1,4,5,6-tetrahydronicotinate + H2O
2-formylglutarate + NH3
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6-oxo-1,4,5,6-tetrahydronicotinate + H2O
2-formylglutarate + NH3
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the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
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r
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6-oxo-1,4,5,6-tetrahydronicotinate + H2O
2-formylglutarate + NH3
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the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
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r
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Fe
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contains 1.0 Fe per subunit. The Fe/Zn binuclear metal center of enamidase catalyzes amide hydrolysis of 6-oxo-1,4,5,6-tetrahydronicotinate, hydration and ammonia elimination
Zn
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contains about 0.6 Zn per subunit
additional information
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the enzyme contains the typical metal binding His-X-His pattern in the N-terminal part
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39793
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4 * 39793, MALDI-TOF MS
40000
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4 * 40000, SDS-PAGE
178000
size-exclusion chromatography
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tetramer
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4 * 39793, MALDI-TOF MS; 4 * 40000, SDS-PAGE
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expression in Escherichia coli strain BL 21
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ENA_EUBBA
386
39923
Swiss-Prot
A0A0M0ELJ7_KOMEU
398
42026
TrEMBL
A0A090EDT9_MESPL
399
41254
TrEMBL
A0A212M0V7_9FIRM
uncultured Sporomusa sp
383
40586
TrEMBL
A0A1Y5RWL3_9PROT
401
41950
TrEMBL
A0A173L018_9SPHN
411
43936
TrEMBL
A0A2H5YIZ2_9BACT
403
41991
TrEMBL
A0A1J5JQR7_MOOTH
387
39664
TrEMBL
A0A1M4M2X0_9FIRM
386
40436
TrEMBL
Q13KU7_PARXL
Paraburkholderia xenovorans (strain LB400)
422
44430
TrEMBL
A0A2U3L9R3_9DELT
388
40243
TrEMBL
A0A090G1M8_MESPL
399
41254
TrEMBL
A0A090SUY3_9VIBR
331
36791
TrEMBL
A0A090GF02_9RHIZ
419
43487
TrEMBL
A0A1Y5RXV6_9RHOB
388
41244
TrEMBL
A0A2H5UXB7_9ARCH
394
41753
TrEMBL
A0A1A0DMA9_ACEPA
446
47540
TrEMBL
A0A2S6QCV0_9PROT
400
43072
TrEMBL
A0A090PAM9_9VIBR
397
44746
TrEMBL
A0A090SV50_9VIBR
270
30935
TrEMBL
A5EEK6_BRASB
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
398
41982
TrEMBL
A0A2R8B775_9RHOB
390
41296
TrEMBL
A0A0U1L0B3_9FIRM
387
39762
TrEMBL
A0A1X6XNY9_9MICO
542
57840
TrEMBL
M7P805_9BACL
411
43918
TrEMBL
A0A2X1BD43_9BORD
397
41778
TrEMBL
A0A0J5TBG0_9RHOB
391
41485
TrEMBL
A0A2S2E1T7_9ALTE
426
45395
TrEMBL
A0A1D7W0K3_BRELN
543
56937
TrEMBL
A0A178WUA5_9PSEU
351
36169
TrEMBL
A0A0H2MND5_VARPD
399
41340
TrEMBL
A0A109LG33_PSEFL
394
41117
TrEMBL
E3H6M1_ILYPC
Ilyobacter polytropus (strain DSM 2926 / CuHBu1)
386
40315
TrEMBL
A0A2S6QCR7_9PROT
396
41447
TrEMBL
A0A212M187_9FIRM
uncultured Sporomusa sp
386
40942
TrEMBL
A0A0T9K7D1_MYCTX
400
40852
TrEMBL
A1WG72_VEREI
Verminephrobacter eiseniae (strain EF01-2)
402
42342
TrEMBL
A0A161WVQ4_9CLOT
386
40441
TrEMBL
A0A174RDM5_9FIRM
325
33948
TrEMBL
A0A1S8TKH6_9CLOT
363
39431
TrEMBL
A0A0K2VTP6_MESPL
399
41241
TrEMBL
A0A238JR73_9RHOB
399
42087
TrEMBL
F4LUP7_TEPAE
Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)
386
40793
TrEMBL
A0A0B8NF40_9NOCA
353
35774
TrEMBL
A0A109KPZ3_PSEFL
394
41215
TrEMBL
A0A1U7MCR6_9FIRM
383
40586
TrEMBL
Q124S7_POLSJ
Polaromonas sp. (strain JS666 / ATCC BAA-500)
401
41743
TrEMBL
A0A090FQ08_MESPL
399
41253
TrEMBL
A0A157R4F6_9BORD
397
41796
TrEMBL
A0A1V6C279_9DELT
290
30263
TrEMBL
A0A259UPG7_9FIRM
388
39727
TrEMBL
A0A1W1B8H8_9ZZZZ
635
71961
TrEMBL
A0A090PDU7_9VIBR
206
22453
TrEMBL
A0A2W0CH71_9BACL
441
48231
TrEMBL
A0A1U7MD02_9FIRM
386
40942
TrEMBL
F4LUP0_TEPAE
Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1)
386
40692
TrEMBL
A0A1L9NTP0_9RHOB
390
41298
TrEMBL
A0A1Y0Y5W0_ACEPA
446
47425
TrEMBL
A0A0H3IZD7_CLOPA
386
40741
TrEMBL
A0A2R8CDA3_9RHOB
389
40373
TrEMBL
A0A1V4W6U0_9DELT
383
40090
TrEMBL
A0A1C5ZXL1_9FIRM
uncultured Flavonifractor sp
395
40962
TrEMBL
Q2IZI1_RHOP2
Rhodopseudomonas palustris (strain HaA2)
398
41378
TrEMBL
A0A0P1FF32_THAGE
387
40906
TrEMBL
A0A2S2DSK5_9BACT
423
45482
TrEMBL
A0A1W1EFX4_9ZZZZ
629
70811
TrEMBL
A0A0B4XE01_9RHIZ
404
42525
TrEMBL
A0A1V5Y808_9FIRM
416
44568
TrEMBL
A0A0T9K7G1_MYCTX
395
41105
TrEMBL
A0A177H7U7_9RHOB
388
40202
TrEMBL
A0A259UL88_9FIRM
387
39860
TrEMBL
A0A1V4WRT4_9DELT
381
39904
TrEMBL
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Alhapel, A.; Darley, D.J.; Wagener, N.; Eckel, E.; Elsner, N.; Pierik, A.J.
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
Eubacterium barkeri
brenda
Kress, D.; Alhapel, A.; Pierik, A.J.; Essen, L.O.
The crystal structure of enamidase: a bifunctional enzyme of the nicotinate catabolism
J. Mol. Biol.
384
837-847
2008
Eubacterium barkeri, Eubacterium barkeri (Q0QLE9)
brenda
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