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Information on EC 3.5.1.19 - nicotinamidase

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.19 nicotinamidase
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UNIPROT: A0A223CGG0 not found.
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
pzase, nicotinamidase, pnc-1, pnc1p, pzaase, nicotinamidase/pyrazinamidase, yndase, as87_01735, nicotinamidase pnc-1, na_as0847, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nicotinamidase/pyrazinamidase PncA
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nicotinamide amidase
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nicotinamide deaminase
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Nicotine deamidase
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YNDase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
nicotinamide amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9033-32-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nicotinamide + H2O
nicotinate + NH3
show the reaction diagram
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A223CGG0_9BACI
183
0
20528
TrEMBL
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
both dimers and tetramers are observed in crystals, but only dimers are observed in solution
tetramer
both dimers and tetramers are observed in crystals, but only dimers are observed in solution
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion method at 14°C. The crystal structure is determined at 2.0 A resolution. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers are observed in crystals, but only dimers are observed in solution
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shang, F.; Chen, J.; Wang, L.; Jin, L.; Zou, L.; Bu, T.; Dong, Y.; Ha, N.C.; Nam, K.H.; Quan, C.; Xu, Y.
Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis
Biochem. Biophys. Res. Commun.
503
2906-2911
2018
Bacillus velezensis (A0A223CGG0)
Manually annotated by BRENDA team