Literature summary for 3.5.1.19 extracted from

Shang, F.; Chen, J.; Wang, L.; Jin, L.; Zou, L.; Bu, T.; Dong, Y.; Ha, N.C.; Nam, K.H.; Quan, C.; Xu, Y.
Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis (2018), Biochem. Biophys. Res. Commun., 503, 2906-2911 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting-drop vapor-diffusion method at 14°C. The crystal structure is determined at 2.0 A resolution. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers are observed in crystals, but only dimers are observed in solution	Bacillus velezensis

Crystallization (Comment)

Organism

sitting-drop vapor-diffusion method at 14°C. The crystal structure is determined at 2.0 A resolution. The structure of BsPncA consists of an alpha/beta domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the alpha/beta domain, while the subdomain is highly flexible. Both dimers and tetramers are observed in crystals, but only dimers are observed in solution

Bacillus velezensis

Organism

Organism	UniProt	Comment	Textmining
Bacillus velezensis	A0A223CGG0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nicotinamide + H2O	-	Bacillus velezensis	nicotinate + NH3	-	?

Subunits

Subunits	Comment	Organism
dimer	both dimers and tetramers are observed in crystals, but only dimers are observed in solution	Bacillus velezensis
tetramer	both dimers and tetramers are observed in crystals, but only dimers are observed in solution	Bacillus velezensis

Synonyms

Synonyms	Comment	Organism
nicotinamidase/pyrazinamidase PncA	-	Bacillus velezensis

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Release 2023.1 (January 2023)