Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.23.12 - nepenthesin and Organism(s) Nepenthes gracilis and UniProt Accession Q766C3

for references in articles please use BRENDA:EC3.4.23.12
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.12 nepenthesin
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Nepenthes gracilis
UNIPROT: Q766C3 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Nepenthes gracilis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg
Synonyms
nepenthesin, nepenthesin i, nepenthesin ii, nepii, nep i, nep ii, aspartylendopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nepenthesin I
isoform
nepenthacin
-
-
-
-
nepenthasin;aspartyl endopeptidase
-
-
-
-
nepenthes aspartic protease
-
-
-
-
Nepenthesin
-
-
-
-
nepenthesin I
-
-
nepenthesin II
isoform
proteinase, nepenthes acid
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9073-80-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aprataxin and PNKP-like factor + H2O
?
show the reaction diagram
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
?
aprataxin and PNKP-like factor + H2O
?
show the reaction diagram
-
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Hemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
guanidine hydrochloride
isoform NepI shows about 50% residual activity at 1 M and is completely inhibited at 4 M guanidine hydrochloride
Urea
isoform NepI shows about 85% residual activity at 4 M and is completely inhibited at 6 M urea
guanidine hydrochloride
isoform NepII shows about 85% residual activity at 4 M, about 50% residual activity at 5 M, and is completely inhibited at 6 M guanidine hydrochloride
pepstatin A
-
-
additional information
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 55
more than 60% activity between 37 and 55°C
45 - 70
more than 60% activity between 45 and 70°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme is the primary constituent of pitcher fluids in the pitcher plant genus Nepenthes and is responsible for the hydrolysis of prey protein
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NEP1_NEPGR
437
0
46357
Swiss-Prot
Secretory Pathway (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with pepstatin A, hanging drop vapor diffusion method, using either 25% (w/v) PEG 3350, 0.1 M glycine pH 2.5 or 2 M ammonium sulfate, 0.1 M glycine pH 2.5, 0.025% (v/v) dichloromethane
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5 - 4
the recombinant isoform NepI retains about 45%, 80%, 99%, 85% and 55% activity after 7 days at pH 1.5, 2.0, 3.0 and 4.0, respectively
752478
1.5 - 4
the recombinant isoform NepII retains about 65%, 90%, 100%, 65% and 30% activity after 7 days at pH 1.5, 2.0, 3.0 and 4.0, respectively
752478
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41(DE3) cells
-
isoform Nep II is expressed in Escherichia coli BL21(DE3) cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the presence of dipteran larvae in the pitcher is correlated with lower fluid proteolytic activity although this effect is absent in pitchers that also have high concentrations of fluid microbes
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fejfarova, K.; Kadek, A.; Mrazek, H.; Hausner, J.; Tretyachenko, V.; Koval, T.; Man, P.; Hasek, J.; Dohnalek, J.
Crystallization of nepenthesin I using a low-pH crystallization screen
Acta Crystallogr. Sect. F
72
24-28
2016
Nepenthes gracilis
Manually annotated by BRENDA team
Yang, M.; Hoeppner, M.; Rey, M.; Kadek, A.; Man, P.; Schriemer, D.C.
Recombinant nepenthesin II for hydrogen/deuterium exchange mass spectrometry
Anal. Chem.
87
6681-6687
2015
Nepenthes gracilis (Q766C2), Nepenthes gracilis (Q766C3)
Manually annotated by BRENDA team
Lam, W.; Chong, K.; Anand, G.; Tan, H.
In situ proteolytic activity in Nepenthes gracilis pitcher plant traps is affected by both pitcher-extrinsic and pitcher-intrinsic factors
Int. J. Plant Sci.
180
179-185
2019
Nepenthes gracilis
-
Manually annotated by BRENDA team