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Literature summary for 3.4.23.12 extracted from

  • Yang, M.; Hoeppner, M.; Rey, M.; Kadek, A.; Man, P.; Schriemer, D.C.
    Recombinant nepenthesin II for hydrogen/deuterium exchange mass spectrometry (2015), Anal. Chem., 87, 6681-6687 .
    View publication on PubMed
Search for more literature for 3.4.23.12

Cloned(Commentary)

Cloned (Comment) Organism
isoform Nep II is expressed in Escherichia coli BL21(DE3) cells Nepenthes gracilis

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride isoform NepII shows about 85% residual activity at 4 M, about 50% residual activity at 5 M, and is completely inhibited at 6 M guanidine hydrochloride; isoform NepI shows about 50% residual activity at 1 M and is completely inhibited at 4 M guanidine hydrochloride Nepenthes gracilis
additional information isoform NepII is not inhibited by urea Nepenthes gracilis
Urea isoform NepI shows about 85% residual activity at 4 M and is completely inhibited at 6 M urea Nepenthes gracilis

Organism

Organism UniProt Comment Textmining
Nepenthes gracilis Q766C2
-
-
Nepenthes gracilis Q766C3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Nepenthes gracilis

Purification (Commentary)

Purification (Comment) Organism
-
 Nepenthes gracilis

Source Tissue

Source Tissue Comment Organism Textmining
pitcher fluid
-
 Nepenthes gracilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aprataxin and PNKP-like factor + H2O
-
 Nepenthes gracilis ?
-
 ?
Bovine serum albumin + H2O
-
 Nepenthes gracilis ?
-
 ?
additional information isoform nepenthesin I has broad cleavage specificity with the exception of cleavage of C-terminal to proline and tryptophan Nepenthes gracilis ?
-
 ?
additional information isoform nepenthesin II shares the broad cleavage specificity of isoform nepenthesin I, but in addition, it cleaves C-terminal to tryptophan Nepenthes gracilis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37497, recombinant mature isoform NepII, MALDI-TOF mass spectrometry Nepenthes gracilis
monomer 1 * 37506, recombinant mature isoform NepII, MALDI-TOF mass spectrometry Nepenthes gracilis
monomer 1 * 50000, recombinant NepII proenzyme, SDS-PAGE Nepenthes gracilis

Synonyms

Synonyms Comment Organism
nepenthesin I isoform Nepenthes gracilis
nepenthesin II isoform Nepenthes gracilis
NepI
-
 Nepenthes gracilis
NEPII
-
 Nepenthes gracilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Nepenthes gracilis
55
-
-
 Nepenthes gracilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 55 more than 60% activity between 37 and 55°C Nepenthes gracilis
45 70 more than 60% activity between 45 and 70°C Nepenthes gracilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 4 the recombinant isoform NepI retains about 45%, 80%, 99%, 85% and 55% activity after 7 days at pH 1.5, 2.0, 3.0 and 4.0, respectively Nepenthes gracilis
1.5 4 the recombinant isoform NepII retains about 65%, 90%, 100%, 65% and 30% activity after 7 days at pH 1.5, 2.0, 3.0 and 4.0, respectively Nepenthes gracilis