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Information on EC 3.4.22.B49 - cathepsin L1 and Organism(s) Schistosoma mansoni and UniProt Accession Q26534

for references in articles please use BRENDA:EC3.4.22.B49
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B49 cathepsin L1
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This record set is specific for:
Schistosoma mansoni
UNIPROT: Q26534
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Word Map
The taxonomic range for the selected organisms is: Schistosoma mansoni
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
clear preference for Arg at P1 position (Lys, Glu, Thr, and Met are less efficient). FheCL1 shows distinct preference for hydrophobic amino acids in the P2, Leu is favored. Cathepsin L1 can accommodate Pro in the P2 position, but less efficiently than cathepsin L2. FheCL1 produces clear degradation fragments from collagen
Synonyms
cathepsin l1, ctsl1, fhcl1, fhecl1, catl1, fgcatl1h, cgctsl1, cpfhw, cathepsin l1h, da-ctsl1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cathepsin L1
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-
-
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cathepsin L1 proteinase
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-
-
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CL1
-
-
-
-
FheCL1
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
60616-82-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Hemoglobin + H2O
?
show the reaction diagram
purified recombinant CL1 is not able to hydrolyze hemoglobin
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Hemoglobin + H2O
?
show the reaction diagram
purified recombinant CL1 is not able to hydrolyze hemoglobin
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CATL_SCHMA
319
0
36136
Swiss-Prot
Chloroplast (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36950
recombinant protein, calculated from amino acid sequence
37000
recombinant protein, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose resin column chromatography, gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-SI cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
mice immunized with recombinant CL1 show a decrease of 21.8% in Schistosoma mansoni burden
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Miysato, P.; Ramos, C.; Abreu, P.; Dias, W.; Nascimento, C.; Ho, P.; Kawano, T.
Expression of schistosomal cathepsin L1 in Escherichia coli and evaluation of its protective capacity in an animal challenge
J. Venom. Anim. Toxins incl. Trop. Dis.
15
289-304
2009
Schistosoma mansoni (Q26534)
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Manually annotated by BRENDA team