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Information on EC 3.2.2.29 - thymine-DNA glycosylase and Organism(s) Pyrobaculum aerophilum and UniProt Accession Q7LX22

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.29 thymine-DNA glycosylase
IUBMB Comments
Thymine-DNA glycosylase is part of the DNA-repair machinery. Thymine removal is fastest when it is from a G/T mismatch with a 5'-flanking C/G pair. The glycosylase removes uracil from G/U, C/U, and T/U base pairs faster than it removes thymine from G/T .
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Pyrobaculum aerophilum
UNIPROT: Q7LX22
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Word Map
The taxonomic range for the selected organisms is: Pyrobaculum aerophilum
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Reaction Schemes
Hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free thymine.
Synonyms
thymine dna glycosylase, thymine-dna glycosylase, mismatch-specific thymine-dna glycosylase, pa-mig, g/t glycosylase, thymine dna-glycosylase, uracil/thymine dna glycosylase, mismatch-specific thymine-dna n-glycosylase, t:g mismatch-specific thymidine-dna glycosylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Pa-MIG
Q9P9L6
-
SYSTEMATIC NAME
IUBMB Comments
thymine-DNA deoxyribohydrolase (thymine-releasing)
Thymine-DNA glycosylase is part of the DNA-repair machinery. Thymine removal is fastest when it is from a G/T mismatch with a 5'-flanking C/G pair. The glycosylase removes uracil from G/U, C/U, and T/U base pairs faster than it removes thymine from G/T [3].
CAS REGISTRY NUMBER
COMMENTARY hide
149565-68-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
thymine-mismatched double-stranded DNA + H2O
thymine + double-stranded DNA with abasic site
show the reaction diagram
Q9P9L6
removes thymine from T/G mismatches
-
-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
show the reaction diagram
Q9P9L6
removes uracil from G/U mismatches, U/7,8-dihydro-oxoguanine and T/7,8-dihydro-oxoguanine mismatches
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4Fe-4S cluster
Q9P9L6
the enzyme contains a binding motif for the 4Fe-4S cluster
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27900
Q9P9L6
mass spectrometry
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
Q9P9L6
10 min, 87% of the protein remains soluble
90
Q9P9L6
10 min, 56% of the protein remains soluble
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Q9P9L6
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, H.; Fitz-Gibbon, S.; Marcotte, E.M.; Tai, J.H.; Hyman, E.C.; Miller, J.H.
Characterization of a thermostable DNA glycosylase specific for U/G and T/G mismatches from the hyperthermophilic archaeon Pyrobaculum aerophilum
J. Bacteriol.
182
1272-1279
2000
Pyrobaculum aerophilum (Q9P9L6), Pyrobaculum aerophilum
Manually annotated by BRENDA team