EC Number   |
|---|
   3.2.2.29 | - |
| 3.2.2.29 | cDNA sequence encoding human TDG (residues 112339) is subcloned into a pGEX4T-3 vector. SUMO-3-modified TDG112339 (SUMO-3TDG) is expressed bacterially using an Escherichia coli SUMOylation system by co-transforming the Escherichia coli strain BL21(DE3) with TDG112339/pGEX4T-3 and pT-E1E2S2 protein expression vectors |
| 3.2.2.29 | glutathione Sepharose column chromatography |
| 3.2.2.29 | GSTrap column chromatography |
| 3.2.2.29 | HiTrap Q column chromatography |
| 3.2.2.29 | Mono Q column chromatography as final chromatographic step |
| 3.2.2.29 | nickel-charged chelating column chromatography, HiTrap SP column chromatography, and Superdex 75 gel filtration |
| 3.2.2.29 | recombinant |
| 3.2.2.29 | recombinant enzyme from NIH3T3 fibroblasts |
| 3.2.2.29 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography |
