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Information on EC 3.2.1.78 - mannan endo-1,4-beta-mannosidase and Organism(s) Niallia circulans and UniProt Accession Q0IJR6

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Niallia circulans
UNIPROT: Q0IJR6 not found.
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The taxonomic range for the selected organisms is: Niallia circulans
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-mannanase, endo-beta-mannanase, man5a, endo-mannanase, manb-1601, endo-beta-1,4-mannanase, man26a, man26b, man5c, endo-1,4-beta-mannanase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,4-beta-D-mannan mannanohydrolase
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-
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beta-1,4-mannan 4-mannanohydrolase
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-
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beta-D-mannanase
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-
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Beta-mannanase
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-
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beta-mannanase B
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-
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endo-1,4-beta-mannanase
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endo-1,4-mannanase
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endo-beta-1,4-mannase
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endo-beta-mannanase
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mannanase, endo-1,4-beta-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-mannan mannanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-54-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
locust bean gum + H2O
?
show the reaction diagram
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76% of products are oligomannosides
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?
guar gum + H2O
?
show the reaction diagram
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26% of the activity with locust bean gum
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?
locust bean gum + H2O
?
show the reaction diagram
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hydrolysis products are mainly oligosaccharides plus one monosaccharide
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?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
1 mM, 124% of initial activity
Co2+
1 mM, 112% of initial activity
Li+
1 mM, 112% of initial activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
strong inhibition
Hg2+
strong inhibition
Sodium dodecyl sulfate
strong inhibition
Ag+
1 mM, 2% residual activity
Cu2+
1 mM, 12% residual activity
Hg2+
1 mM, 8% residual activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4839
pH 7.6, 60°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
more than 75% of maximum activity within
6.8 - 8
more than 75% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q0IJR6_NIACI
326
1
35215
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
x * 32000, calculated and SDS-PAGE
31000
x * 31000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 32000, calculated and SDS-PAGE
?
x * 31000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
retains more than 75% of maximum activity after 1 h incubation
695697
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
60 min, 90% residual activity
60
20 min, complete loss of activity
50
retains 90% of its activity after incubation for 60 min
60
but no activity remains after incubation for 20 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is resistant to digestion by trypsin
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris and Escherichia coli
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, P.; Li, Y.; Wang, Y.; Meng, K.; Luo, H.; Yuan, T.; Bai, Y.; Zhan, Z.; Yao, B.
A novel beta-mannanase with high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and enzymatic characterization
Appl. Biochem. Biotechnol.
159
85-94
2008
Niallia circulans (Q0IJR6), Niallia circulans CGMCC1554 (Q0IJR6)
Manually annotated by BRENDA team
Li, Y.; Yang, P.; Meng, K.; Wang, Y.; Luo, H.; Wu, N.; Fan, Y.; Yao, B.
Gene cloning, expression, and characterization of a novel beta-mannanase from Bacillus circulans CGMCC 1416
J. Microbiol. Biotechnol.
18
160-166
2008
Niallia circulans (Q49HI4), Niallia circulans, Niallia circulans CGMCC 1416 (Q49HI4)
Manually annotated by BRENDA team