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Information on EC 3.2.1.22 - alpha-galactosidase and Organism(s) Saccharolobus solfataricus and UniProt Accession Q97U94

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IUBMB Comments
Also hydrolyses alpha-D-fucosides.
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This record set is specific for:
Saccharolobus solfataricus
UNIPROT: Q97U94
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Word Map
The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-galactosidase, alpha-galactosidase a, alpha-gal, alpha-gal a, agalsidase alfa, genzyme, alpha-d-galactosidase, a-galactosidase, fabrazyme, alpha-galactosidase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Agalsidase alfa
-
-
-
-
alpha-D-galactosidase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactoside galactohydrolase
-
-
-
-
ceramidase, galactosylgalactosylglucosyl-
-
-
-
-
ceramide trihexosidase
-
-
-
-
ceramidetrihexosidase
-
-
-
-
ceramidetrihexoside-alpha-galactosidase
-
-
-
-
melibiase
-
-
-
-
trihexosyl ceramide galactosidase
-
-
-
-
trihexosylceramide alpha-galactosidase
-
-
-
-
trihexosylceramidealpha-galactosidase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-01-2
-
9025-35-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-L-arabinopyranoside + H2O
p-nitrophenol + beta-L-arabinose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag2+
complete inhibition at 10 mM
Ca2+
complete inhibition at 10 mM
Cd2+
complete inhibition at 10 mM
Co2+
complete inhibition at 10 mM
Cu2+
complete inhibition at 10 mM
Hg2+
complete inhibition at 10 mM
Mn2+
complete inhibition at 10 mM
Ni2+
complete inhibition at 10 mM
Zn2+
complete inhibition at 10 mM
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.085
p-nitrophenyl alpha-D-galactopyranoside
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
37.4
p-nitrophenyl-beta-L-arabinopyranoside
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.18
crude cell extract, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
4.25
after 23fold purification, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
225000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.1 - 6.7
more than 50% active between pH 4.1 and 6.7
680458
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
completely inactive at temperatures below 50°C
75
50% active at 75°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose Fast Flow column chromatography, hydroxyapatite column chromatography, Mono Q 5/50 GL column chromatography, and Superdex 200 HR 10/30 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21(DE3)-RIL
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Brouns, S.J.; Smits, N.; Wu, H.; Snijders, A.P; Wright, P.C.; de Vos, W.M.; van der Oost, J.
Identification of a novel alpha-galactosidase from the hyperthermophilic archaeon Sulfolobus solfataricus.
J. Bacteriol.
188
2392-2399
2006
Saccharolobus solfataricus (Q97U94), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97U94)
Manually annotated by BRENDA team