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Information on EC 3.2.1.156 - oligosaccharide reducing-end xylanase and Organism(s) Bifidobacterium adolescentis and UniProt Accession A1A048

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IUBMB Comments
The enzyme, originally isolated from the bacterium Bacillus halodurans C-125, releases the xylose unit at the reducing end of oligosaccharides ending with the structure beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose, leaving the new reducing end in the alpha configuration. It is specific for the beta anomers of xylooligosaccharides whose degree of polymerization is equal to or greater than 3. The penultimate residue must be beta-D-xylopyranose, but replacing either of the flanking residues with glucose merely slows the rate greatly.
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Bifidobacterium adolescentis
UNIPROT: A1A048
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The taxonomic range for the selected organisms is: Bifidobacterium adolescentis
The enzyme appears in selected viruses and cellular organisms
Synonyms
rex8a, reducing-end xylose-releasing exo-oligoxylanase, rxyn8, phxyl, reducing end xylose-releasing exo-oligoxylanase, oligosaccharide reducing-end xylanase, gh8 xylanase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oligosaccharide reducing-end xylanase
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reducing-end xylose-releasing exo-oligoxylanase
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reducing-end xylose-releasing exooligoxylanase
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SYSTEMATIC NAME
IUBMB Comments
beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose reducing-end xylanase
The enzyme, originally isolated from the bacterium Bacillus halodurans C-125, releases the xylose unit at the reducing end of oligosaccharides ending with the structure beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranose, leaving the new reducing end in the alpha configuration. It is specific for the beta anomers of xylooligosaccharides whose degree of polymerization is equal to or greater than 3. The penultimate residue must be beta-D-xylopyranose, but replacing either of the flanking residues with glucose merely slows the rate greatly.
CAS REGISTRY NUMBER
COMMENTARY hide
55126-95-9
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879497-03-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrohenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl + H2O
2-nitrophenol + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
show the reaction diagram
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-
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?
arabinofuranosyl-xylotriose + H2O
D-xylose + xylobiose + ?
show the reaction diagram
good substrate
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?
xylan + H2O
?
show the reaction diagram
beechwood xylan or wheat flour arabinoxylan, low activity
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-
?
xylooligosaccharides + H2O
D-xylose + Xylbeta(1-4)Xyl
show the reaction diagram
-
-
-
?
xylopentaose + H2O
xylotetraose + xylobiose
show the reaction diagram
the recombinant enzyme constitutively hydrolyzes a xylose residue from Xn when n is above 2, until the final products are (n-2)X1 and X2
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?
xylotetraose + H2O
xylotriose + xylobiose
show the reaction diagram
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-
-
?
xylotriose + H2O
D-xylose + xylobiose
show the reaction diagram
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
show the reaction diagram
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-
-
-
?
2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
show the reaction diagram
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?
2-nitrophenyl xylohexaose + H2O
?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
Rex8As are key enzymes in the efficient saccharification of heteroxylan into xylose, a major component of lignocellulosic substrates
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
RexA is stable at temperatures up to 40°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme is stable for at least 4 months at 4°C in 25 mM sodium acetate buffer, pH 5.0
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by using a single-step HisTrap purification performed on a Atka fast protein liquid chromatograph. Protein yield: 140 mg recombinant enzyme per liter cell culture
Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
expressed in Escherichia coli BL21(DE3)pLysS cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lagaert, S.; Van Campenhout, S.; Pollet, A.; Bourgois, T.M.; Delcour, J.A.; Courtin, C.M.; Volckaert, G.
Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis
Appl. Environ. Microbiol.
73
5374-5377
2007
Bifidobacterium adolescentis (A1A048), Bifidobacterium adolescentis
Manually annotated by BRENDA team
Pollet, A.; Schoepe, J.; Dornez, E.; Strelkov, S.; Delcour, J.; Courtin, C.
Functional analysis of glycoside hydrolase family 8 xylanases shows narrow but distinct substrate specificities and biotechnological potential
Appl. Microbiol. Biotechnol.
87
2125-2135
2010
Bifidobacterium adolescentis, Halalkalibacterium halodurans, Pseudoalteromonas haloplanktis, uncultured bacterium
Manually annotated by BRENDA team
Lagaert, S.; Pollet, A.; Delcour, J.A.; Lavigne, R.; Courtin, C.M.; Volckaert, G.
Characterization of two beta-xylosidases from Bifidobacterium adolescentis and their contribution to the hydrolysis of prebiotic xylooligosaccharides
Appl. Microbiol. Biotechnol.
92
1179-1185
2011
Bifidobacterium adolescentis (A1A048), Bifidobacterium adolescentis
Manually annotated by BRENDA team
Malgas, S.; Pletschke, B.I.
The effect of an oligosaccharide reducing-end xylanase, BhRex8A, on the synergistic degradation of xylan backbones by an optimised xylanolytic enzyme cocktail
Enzyme Microb. Technol.
122
74-81
2019
Bifidobacterium adolescentis (A1A048), Bifidobacterium adolescentis, Bifidobacterium adolescentis ATCC 15703 (A1A048), Bifidobacterium adolescentis DSM 20083 (A1A048), Bifidobacterium adolescentis E194a (A1A048), Bifidobacterium adolescentis NCTC 11814 (A1A048), Halalkalibacterium halodurans (Q9KB30), Halalkalibacterium halodurans DSM 181973 (Q9KB30)
Manually annotated by BRENDA team