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Literature summary for 3.2.1.156 extracted from
- Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis (2007), Appl. Environ. Microbiol., 73, 5374-5377.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-tagged fusion protein in Escherichia coli | Bifidobacterium adolescentis |
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme is stable for at least 4 months at 4°C in 25 mM sodium acetate buffer, pH 5.0 | Bifidobacterium adolescentis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
SDS-PAGE | Bifidobacterium adolescentis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium adolescentis | A1A048 | putative | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by using a single-step HisTrap purification performed on a Atka fast protein liquid chromatograph. Protein yield: 140 mg recombinant enzyme per liter cell culture | Bifidobacterium adolescentis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylopentaose + H2O | the recombinant enzyme constitutively hydrolyzes a xylose residue from Xn when n is above 2, until the final products are (n-2)X1 and X2 | Bifidobacterium adolescentis | xylotetraose + xylobiose | - |
? |  |
| xylotetraose + H2O | - |
Bifidobacterium adolescentis | xylotriose + xylobiose | - |
? | |
| xylotriose + H2O | - |
Bifidobacterium adolescentis | D-xylose + xylobiose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RexA | - |
Bifidobacterium adolescentis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bifidobacterium adolescentis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
RexA is stable at temperatures up to 40°C | Bifidobacterium adolescentis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bifidobacterium adolescentis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Bifidobacterium adolescentis |
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Release 2023.1 (January 2023)
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