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Information on EC 3.2.1.130 - glycoprotein endo-alpha-1,2-mannosidase

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EC Tree
IUBMB Comments
The enzyme catalyses the hydrolysis of the terminal alpha-D-glucosyl-(1->3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al .
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This record set is specific for:
UNIPROT: Q8A109
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
endomannosidase, manea, endo-alpha-d-mannosidase, endo-alpha-mannosidase, golgi-endomannosidase, alpha-endomannosidase, glucosyl mannosidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endo-alpha-mannosidase
-
endo-alpha-D-mannosidase
-
-
-
-
endo-alpha-mannosidase
-
-
-
-
endomannosidase
-
-
-
-
glucosyl mannosidase
-
-
-
-
glucosylmannosidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
glycoprotein glucosylmannohydrolase
The enzyme catalyses the hydrolysis of the terminal alpha-D-glucosyl-(1->3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al [7].
CAS REGISTRY NUMBER
COMMENTARY hide
108022-16-8
-
125858-79-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride + H2O
?
show the reaction diagram
-
-
-
?
Glc(1->3)Man9GlcNAc2 + H2O
Man8GlcNAc + glucosyl alpha(1->3)-mannose
show the reaction diagram
-
-
-
?
GlcMan7GlcNAc2 + H2O
Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
show the reaction diagram
-
-
-
?
GlcMan9GlcNAc2 + H2O
?
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GlcMan7GlcNAc2 + H2O
Man6GlcNAc2 + glucosyl alpha(1->3)-D-mannose
show the reaction diagram
-
-
-
?
GlcMan9GlcNAc2 + H2O
?
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3R,4R,5R)-3-(alpha-D-glucopyranosyloxy)-4-hydroxy-5-(hydroxymethyl)piperidine
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alpha-glucopyranosyl-(1->3)-deoxymannojirimycin
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.48 - 2.6
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58 - 4.77
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 9.9
alpha-glucopyranosyl-(1->3)-alpha-mannopyranosyl fluoride
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8A109_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
382
0
43215
TrEMBL
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
single wavelength anomalous dispersion technique
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E154A
the mutant shows near zero activity
E329A
the mutant shows near zero activity
E332A
the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
NiSO4-charged HiTrap column chromatography and S75 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thompson, A.J.; Williams, R.J.; Hakki, Z.; Alonzi, D.S.; Wennekes, T.; Gloster, T.M.; Songsrirote, K.; Thomas-Oates, J.E.; Wrodnigg, T.M.; Spreitz, J.; Stuetz, A.E.; Butters, T.D.; Williams, S.J.; Davies, G.J.
Structural and mechanistic insight into N-glycan processing by endo-alpha-mannosidase
Proc. Natl. Acad. Sci. USA
109
781-786
2012
Bacteroides thetaiotaomicron (Q8A109), Bacteroides thetaiotaomicron DSM 2079 (Q8A109), Bacteroides xylanisolvens (D6D1V7), Bacteroides xylanisolvens XB1A (D6D1V7)
Manually annotated by BRENDA team