Information on EC 3.2.1.115 - branched-dextran exo-1,2-alpha-glucosidase

for references in articles please use BRENDA:EC3.2.1.115
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The expected taxonomic range for this enzyme is: Flavobacterium sp.

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EC NUMBER
COMMENTARY hide
3.2.1.115
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RECOMMENDED NAME
GeneOntology No.
branched-dextran exo-1,2-alpha-glucosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->2)-alpha-D-glucosidic linkages at the branch points of dextrans and related polysaccharides, producing free D-glucose
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
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hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
(1->2)-alpha-D-glucosyl-branched-dextran 2-glucohydrolase
Does not hydrolyse disaccharides or oligosaccharides containing linear 1,2-alpha-glucosidic linkages.
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CAS REGISTRY NUMBER
COMMENTARY hide
72840-94-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2(3),2(4)-di-O-alpha-glucosyl-isomaltotetraose + H2O
isomaltotetraose + 2 alpha-D-glucose
show the reaction diagram
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-
-
-
?
2(3)-O-alpha-D-glucosyl-isomaltotetraose + H2O
isomaltotetraose + alpha-D-glucose
show the reaction diagram
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-
-
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?
2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O
isomaltotriose + alpha-D-glucose
show the reaction diagram
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-
-
-
?
B-1298 soluble dextran + H2O
D-glucose + ?
show the reaction diagram
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incubation at 40°C for 24 h, specifically splitz alpha-1,2-glucosidic linkages
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-
?
B-1299 soluble dextran + H2O
D-glucose + ?
show the reaction diagram
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incubation at 40°C for 24 h
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-
?
B-1397 soluble dextran + H2O
D-glucose + ?
show the reaction diagram
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incubation at 40°C for 24 h
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-
?
dextran + H2O
alpha-D-glucose
show the reaction diagram
mutan IG-1 + H2O
?
show the reaction diagram
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major linkages: alpha-1,3-, alpha-1,6-, 18% as effective as soluble dextran for induction of enzyme
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
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1.33 mM, 0.9% relative activity
Cu2+
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1.33 mM, 1.7% relative activity
D-glucono-1,5-lactone
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strong inhibitor, 34.2% relative activity
Fe3+
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1.33 mM, 2.6% relative activity
Hg2+
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1.33 mM, 1.2% relative activity
iodoacetic acid
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still 60.1% relative activity
Sodium dodecyl sulfate
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strong inhibitor, 37.9% relative activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
effect of different carbon sources on enzyme activity, inducibility of enzyme
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
B-1298 soluble dextran
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mg/ml
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0.27
B-1299 soluble dextran
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mg/ml
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0.91
B-1397 soluble dextran
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mg/ml
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66
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pH 5.6, 40°C
additional information
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5.8 micro-g glucose/ml, substrate B-1298 soluble dextran; 5.8 micro-g glucose/ml, substrate B-1299 soluble dextran; 5.8 micro-g glucose/ml, substrate B-1397 soluble dextran
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
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assay at
6
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highest activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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assay at
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
125000
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 125000, SDS-PAGE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
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incubation at 4°C for 24 h
697134
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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up to 40°C on heating for 10 min
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.115)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)