Information on EC 3.2.1.115 - branched-dextran exo-1,2-alpha-glucosidase

for references in articles please use BRENDA:EC3.2.1.115
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Flavobacterium sp.

top print hide
EC NUMBER
COMMENTARY hide
3.2.1.115
-
top print hide
RECOMMENDED NAME
GeneOntology No.
branched-dextran exo-1,2-alpha-glucosidase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->2)-alpha-D-glucosidic linkages at the branch points of dextrans and related polysaccharides, producing free D-glucose
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
-
hydrolysis of O-glycosyl bond
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
(1->2)-alpha-D-glucosyl-branched-dextran 2-glucohydrolase
Does not hydrolyse disaccharides or oligosaccharides containing linear 1,2-alpha-glucosidic linkages.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
72840-94-9
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2(3),2(4)-di-O-alpha-glucosyl-isomaltotetraose + H2O
isomaltotetraose + 2 alpha-D-glucose
show the reaction diagram
-
-
-
-
?
2(3)-O-alpha-D-glucosyl-isomaltotetraose + H2O
isomaltotetraose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O
isomaltotriose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
B-1298 soluble dextran + H2O
D-glucose + ?
show the reaction diagram
-
incubation at 40°C for 24 h, specifically splitz alpha-1,2-glucosidic linkages
-
-
?
B-1299 soluble dextran + H2O
D-glucose + ?
show the reaction diagram
-
incubation at 40°C for 24 h
-
-
?
B-1397 soluble dextran + H2O
D-glucose + ?
show the reaction diagram
-
incubation at 40°C for 24 h
-
-
?
dextran + H2O
alpha-D-glucose
show the reaction diagram
mutan IG-1 + H2O
?
show the reaction diagram
-
major linkages: alpha-1,3-, alpha-1,6-, 18% as effective as soluble dextran for induction of enzyme
-
-
?
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
hydrolysis of alpha-1,2-glucosidic linkages at branching points of dextran
-
-
-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
-
1.33 mM, 0.9% relative activity
Cu2+
-
1.33 mM, 1.7% relative activity
D-glucono-1,5-lactone
-
strong inhibitor, 34.2% relative activity
Fe3+
-
1.33 mM, 2.6% relative activity
Hg2+
-
1.33 mM, 1.2% relative activity
iodoacetic acid
-
still 60.1% relative activity
Sodium dodecyl sulfate
-
strong inhibitor, 37.9% relative activity
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
effect of different carbon sources on enzyme activity, inducibility of enzyme
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
B-1298 soluble dextran
-
mg/ml
-
0.27
B-1299 soluble dextran
-
mg/ml
-
0.91
B-1397 soluble dextran
-
mg/ml
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66
-
pH 5.6, 40°C
additional information
-
5.8 micro-g glucose/ml, substrate B-1298 soluble dextran; 5.8 micro-g glucose/ml, substrate B-1299 soluble dextran; 5.8 micro-g glucose/ml, substrate B-1397 soluble dextran
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
assay at
6
-
highest activity
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
assay at
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
125000
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 125000, SDS-PAGE
top print hide
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
incubation at 4°C for 24 h
697134
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
up to 40°C on heating for 10 min
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.115)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)