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EC Tree
IUBMB Comments The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine
+
=
+
[collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
Synonyms
2-O-alpha-D-glucopyranosyl-5-O-beta-D-galactopyranosylhydroxy-L-lysine glucohydrolase, 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase,
ATHL1 , GGHG, glucosidase, glycosylgalactosylhydroxylysine, glycosyl-galactosyl-hydroxylysine glucosidase, glycosylgalactosylhydroxylysine glucosidase, PGGHG,
more
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2-O-alpha-D-glucopyranosyl-5-O-beta-D-galactopyranosylhydroxy-L-lysine glucohydrolase
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2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase
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glucosidase, glycosylgalactosylhydroxylysine
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glycosyl-galactosyl-hydroxylysine glucosidase
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glycosylgalactosylhydroxylysine glucosidase
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[collagen]-(5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy-L-lysine + H2O = D-glucose + [collagen]-(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysine
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hydrolysis of O-glycosyl bond
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protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
basement membrane
D-glucose + ?
-
-
-
?
kojibiose + H2O
alpha-D-glucose
maltose + H2O
alpha-D-glucose
Type IV collagen + H2O
?
the enzyme cleaves glucose from disaccharide unit (Glc-alpha-(1,2)-Gal) linked to hydroxylysine residues of collagen
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?
additional information
?
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?
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
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N-acetylated
-
?
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
-
-
-
-
?
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
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-
-
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?
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
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?
isomaltose + H2O
?
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?
isomaltose + H2O
?
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?
kojibiose + H2O
alpha-D-glucose
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?
kojibiose + H2O
alpha-D-glucose
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?
maltose + H2O
alpha-D-glucose
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-
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?
maltose + H2O
alpha-D-glucose
-
-
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-
?
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D-glucono-1,5-lactone
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10 mM complete inhibition
NaCl
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0.8 mM 62% inhibition
Cu2+
-
1 mM
glucosamine
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glucosamine
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competitive
p-chloromercuribenzoate
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p-chloromercuribenzoate
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4 micromol complete inhibition
p-chloromercuribenzoate
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1 mM complete inhibition
Zn2+
-
1 mM
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2.2 - 5.9
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine
2.2
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine
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-
5.7
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine
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5.9
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine
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-
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additional information
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highest activity in the kidneys of neonatal rats and decreases with age
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3.9 - 5.8
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pH 3.9 and 5.8, 50% of maximal activity
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male albino rat Charles River CD strain
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brenda
Sprague-Dawley
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brenda
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SwissProt
brenda
White Leghorns
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brenda
female, normale and diabetic mal Wistar rats
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brenda
male albino rat Charles River CD strain
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brenda
Sprague-Dawley
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brenda
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PGGHG_CHICK
702
0
78358
Swiss-Prot
other Location (Reliability: 2 )
PGGHG_DANRE
655
0
72439
Swiss-Prot
other Location (Reliability: 2 )
PGGHG_DICDI
713
0
80522
Swiss-Prot
other Location (Reliability: 2 )
PGGHG_HUMAN
737
0
80655
Swiss-Prot
other Location (Reliability: 1 )
PGGHG_MOUSE
690
0
76488
Swiss-Prot
other Location (Reliability: 1 )
A0A6J8CDI5_MYTCO
707
0
79295
TrEMBL
Secretory Pathway (Reliability: 4 )
A0A6J8CLS0_MYTCO
702
0
79882
TrEMBL
other Location (Reliability: 5 )
A0A5P9QE66_9MICO
1274
1
135029
TrEMBL
-
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D301E
mutations leads to complete elimination of enzyme activity
D301N
mutations leads to complete elimination of enzyme activity
E430D
mutations leads to complete elimination of enzyme activity
E430Q
mutations leads to complete elimination of enzyme activity
E574D
mutations leads to complete elimination of enzyme activity
E574Q
mutations leads to complete elimination of enzyme activity
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15 mg/l bovine serum albumin stabilize
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ATHL1 cDNA is cloned to a cloning and expression vector and two plasmid clones with different ATHL1 CDS insert are obtained. Each plasmid DNA is transformed into Escherichia coli cells for expression and two isoforms of chicken PGGHG are obtained. Both isoforms effectively released glucose from type IV collagen
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Hamazaki, H.; Hotta, K.
Purification and characterization of an alpha-glucosidase specific for hydroxylysine-linked disaccharide of collagen
J. Biol. Chem.
254
9682-9687
1979
Gallus gallus
brenda
Hamazaki, H.; Hotta, K.
Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens
Eur. J. Biochem.
111
587-591
1980
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
brenda
Sternberg, M.; Spiro, R.G.
Studies on the catabolism of the hydroxylysine-linked disaccharide units of basement membranes and collagens. Isolation and characterization of a rat kidney alpha-glucosidase of high specificity
J. Biol. Chem.
254
10329-10336
1979
Rattus norvegicus, Rattus norvegicus Charles River CD
brenda
Grigorova-Boros, A.M.; Tacnet, F.; Fischer, R.W.; Winterhalter, K.H.; Sternberg, M.
Studies on the catabolism of glycated haemoglobin. Glycated globin is not a substrate for the glycosyl-galactosyl-hydroxylysine glucosidase but for a peptidase activity present in rat kidney and spleen
Amino Acids
4
63-72
1993
Rattus norvegicus
brenda
Hamazaki, H.; Hamazaki, M.
Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis
Biochem. Biophys. Res. Commun.
469
357-362
2016
Gallus gallus (F1NZI4), Gallus gallus
brenda
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