Information on EC 3.2.1.107 - protein-glucosylgalactosylhydroxylysine glucosidase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
3.2.1.107
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RECOMMENDED NAME
GeneOntology No.
protein-glucosylgalactosylhydroxylysine glucosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protein alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine + H2O = D-glucose + protein beta-D-galactosyl-L-hydroxylysine
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
procollagen hydroxylation and glycosylation
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SYSTEMATIC NAME
IUBMB Comments
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
The enzyme specifically hydrolyses glucose from alpha-D-glucosyl-(1->2)-beta-D-galactosyl disaccharide units that are linked to hydroxylysine residues of collagen and collagen-like proteins. Acetylation of the epsilon-amino group of the glycosylated hydroxylysine abolishes activity.
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CAS REGISTRY NUMBER
COMMENTARY hide
72829-45-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
White Leghorns
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Manually annotated by BRENDA team
male albino rat Charles River CD strain
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Manually annotated by BRENDA team
Sprague-Dawley
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
show the reaction diagram
basement membrane
D-glucose + ?
show the reaction diagram
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-
?
isomaltose + H2O
?
show the reaction diagram
kojibiose + H2O
alpha-D-glucose
show the reaction diagram
maltose + H2O
alpha-D-glucose
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucono-1,5-lactone
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10 mM complete inhibition
glucosamine
iodoacetic acid
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NaCl
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0.8 mM 62% inhibition
p-chloromercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2 - 5.9
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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highest activity in the kidneys of neonatal rats and decreases with age
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4 - 4.7
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-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.9 - 5.8
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pH 3.9 and 5.8, 50% of maximal activity
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
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gel filtration
75000
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SDS-PAGE
85000
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SDS-PAGE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
15 mg/l bovine serum albumin stabilize
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.107)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)