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EC Tree
IUBMB Comments A group of enzymes with varying specificity for the monoterpenol moiety. One has the highest activity on sterically hindered compounds such as (+)-bornyl diphosphate; another has highest activity on the diphosphates of primary allylic alcohols such as geraniol.
The enzyme appears in viruses and cellular organisms
Synonyms
bornyl pyrophosphate hydrolase, BPP hydrolase, monoterpenyl-pyrophosphatase, pyrophosphate hydrolase,
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bornyl pyrophosphate hydrolase
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monoterpenyl-pyrophosphatase
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pyrophosphate hydrolase
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a monoterpenyl diphosphate + H2O = a monoterpenol + diphosphate
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hydrolysis of phosphoric ester
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monoterpenyl-diphosphate diphosphohydrolase
A group of enzymes with varying specificity for the monoterpenol moiety. One has the highest activity on sterically hindered compounds such as (+)-bornyl diphosphate; another has highest activity on the diphosphates of primary allylic alcohols such as geraniol.
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D-bornyl-diphosphate + H2O
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camphor biosynthesis
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?
D-bornyl-diphosphate + H2O
D-borneol + diphosphate
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?
D-bornyl-phosphate + H2O
D-borneol + phosphate
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?
geranyl-diphosphate + H2O
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acyclic precursor of camphor
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?
geranyl-diphosphate + H2O
geraniol + diphosphate
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preference for geranyl-ester for 65 kDa BPP hydrolase
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?
neryl-diphosphate + H2O
nerol + diphosphate
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?
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D-bornyl-diphosphate + H2O
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camphor biosynthesis
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?
geranyl-diphosphate + H2O
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acyclic precursor of camphor
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?
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Mg2+
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slight stimulation at 10 mM
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diisopropylfluorophosphate
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IC50: 0.8 for 65 kDa BPP hydrolase
diphosphate
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IC50: 0.6 for 65 kDa BPP hydrolase, 2.5 for 20 kDa BPP hydrolase
F-
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IC50: 0.7 mM for 65 kDa BPP hydrolase, 15.5 mM for 20 kDa BPP hydrolase
Mn2+
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IC50: 0.1 mM for 65 kDa BPP hydrolase, 15.0 mM for 20 kDa BPP hydrolase
phosphate
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IC50: 10.5 mM for 65 kDa BPP hydrolase, 4.3 for 20 kDa BPP hydrolase
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0.023 - 0.12
D-bornyl-diphosphate
0.023
D-bornyl-diphosphate
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65 kDa BPP hydrolase
0.12
D-bornyl-diphosphate
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20 kDa BPP hydrolase
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0.8
diisopropylfluorophosphate
Salvia officinalis
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IC50: 0.8 mM for 65 kDa BPP hydrolase
0.7
F-
Salvia officinalis
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IC50: 0.7 mM for 65 kDa BPP hydrolase, 15.5 mM for 20 kDa BPP hydrolase
0.1
Mn2+
Salvia officinalis
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IC50: 0.1 mM for 65 kDa BPP hydrolase, 15.0 mM for 20 kDa BPP hydrolase
10.5
phosphate
Salvia officinalis
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IC50: 10.5 mM for 65 kDa BPP hydrolase, 4.3 for 20 kDa BPP hydrolase
0.6
diphosphate
Salvia officinalis
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IC50: 0.6 mM for 65 kDa BPP hydrolase
2.5
diphosphate
Salvia officinalis
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IC50 2.5 mM for 20 kDa BPP hydrolase
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3.5
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65 kDa BPP hydrolase
7.6
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20 kDa BPP hydrolase
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2.5 - 4.5
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65 kDa BPP hydrolase, 33% of maximum activity above pH 4.5 and below pH 2.5
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sage
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brenda
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brenda
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J9PZR5_CATRO
589
0
67728
TrEMBL
Chloroplast (Reliability: 2 )
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20000
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gel filtration, activity on bornyl-diphosphate and bornyl-monophosphate
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0°C, 100 mM Tris-maleate pH 7.0, one week, 50% loss of activity for 20 kDa BPP hydrolase
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0°C, 100 mM Tris-maleate pH 7.0, one week, less than 15% loss of activity for 65 kDa BPP hydrolase
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first step: gel filtration, second step: hydroxylapatite chromatography
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Croteau R.; Karp, F.
Biosynthesis of monoterpenes: Hydrolysis of bornyl pyrophosphate, an essential step in camphor biosynthesis, and hydrolysis of geranyl pyrophosphate, the acyclic precursor of camphor, by enzymes from Sage (Salvia officinalis)
Arch. Biochem. Biophys.
198
523-532
1979
Salvia officinalis
brenda
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