Information on EC 3.1.7.3 - monoterpenyl-diphosphatase

for references in articles please use BRENDA:EC3.1.7.3
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The expected taxonomic range for this enzyme is: Salvia officinalis

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EC NUMBER
COMMENTARY hide
3.1.7.3
-
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RECOMMENDED NAME
GeneOntology No.
monoterpenyl-diphosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a monoterpenyl diphosphate + H2O = a monoterpenol + diphosphate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Monoterpenoid biosynthesis
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-
Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
monoterpenyl-diphosphate diphosphohydrolase
A group of enzymes with varying specificity for the monoterpenol moiety. One has the highest activity on sterically hindered compounds such as (+)-bornyl diphosphate; another has highest activity on the diphosphates of primary allylic alcohols such as geraniol.
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CAS REGISTRY NUMBER
COMMENTARY hide
72668-82-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
sage
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-bornyl-diphosphate + H2O
?
show the reaction diagram
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camphor biosynthesis
-
-
-
D-bornyl-diphosphate + H2O
D-borneol + diphosphate
show the reaction diagram
-
-
-
?
D-bornyl-phosphate + H2O
D-borneol + phosphate
show the reaction diagram
-
-
-
?
geranyl-diphosphate + H2O
?
show the reaction diagram
-
acyclic precursor of camphor
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-
-
geranyl-diphosphate + H2O
geraniol + diphosphate
show the reaction diagram
-
preference for geranyl-ester for 65 kDa BPP hydrolase
-
?
neryl-diphosphate + H2O
nerol + diphosphate
show the reaction diagram
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-bornyl-diphosphate + H2O
?
show the reaction diagram
-
camphor biosynthesis
-
-
-
geranyl-diphosphate + H2O
?
show the reaction diagram
-
acyclic precursor of camphor
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
slight stimulation at 10 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diisopropylfluorophosphate
-
IC50: 0.8 for 65 kDa BPP hydrolase
diphosphate
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IC50: 0.6 for 65 kDa BPP hydrolase, 2.5 for 20 kDa BPP hydrolase
F-
-
IC50: 0.7 mM for 65 kDa BPP hydrolase, 15.5 mM for 20 kDa BPP hydrolase
Mn2+
-
IC50: 0.1 mM for 65 kDa BPP hydrolase, 15.0 mM for 20 kDa BPP hydrolase
phosphate
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IC50: 10.5 mM for 65 kDa BPP hydrolase, 4.3 for 20 kDa BPP hydrolase
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023 - 0.12
D-bornyl-diphosphate
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
diisopropylfluorophosphate
Salvia officinalis;
-
IC50: 0.8 mM for 65 kDa BPP hydrolase
0.6 - 2.5
diphosphate
0.7
F-
Salvia officinalis;
-
IC50: 0.7 mM for 65 kDa BPP hydrolase, 15.5 mM for 20 kDa BPP hydrolase
0.1
Mn2+
Salvia officinalis;
-
IC50: 0.1 mM for 65 kDa BPP hydrolase, 15.0 mM for 20 kDa BPP hydrolase
10.5
phosphate
Salvia officinalis;
-
IC50: 10.5 mM for 65 kDa BPP hydrolase, 4.3 for 20 kDa BPP hydrolase
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
65 kDa BPP hydrolase
7.6
-
20 kDa BPP hydrolase
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 4.5
-
65 kDa BPP hydrolase, 33% of maximum activity above pH 4.5 and below pH 2.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
gel filtration, activity on bornyl-diphosphate and bornyl-monophosphate
65000
-
gel filtration
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 100 mM Tris-maleate pH 7.0, one week, 50% loss of activity for 20 kDa BPP hydrolase
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0°C, 100 mM Tris-maleate pH 7.0, one week, less than 15% loss of activity for 65 kDa BPP hydrolase
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
first step: gel filtration, second step: hydroxylapatite chromatography
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.7.3)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)