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IUBMB Comments Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known .
The taxonomic range for the selected organisms is: Mus musculus The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
inositol monophosphatase, impase, inositol phosphatase, impa2, impa1, myo-inositol monophosphatase, inpp5f, impp, myo-inositol-1-phosphatase, inositol-1-phosphatase,
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inositol 1-phosphate phosphatase
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inositol monophosphate phosphatase
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inositol phosphatase
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inositol phosphohydrolase
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inositol-1(or 4)-monophosphatase
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inositol-1-phosphatase
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L-myo-inositol-1-phosphate phosphatase
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Lithium-sensitive myo-inositol monophosphatase A1
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myo-inositol 1 (or 4) -monophosphatase
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myo-inositol 1-phosphatase
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myo-inositol monophosphatase
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Myo-inositol monophosphatase A2
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myo-inositol-1(or 4)-phosphate phosphohydrolase
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IMPase
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inositol monophosphatase
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inositol monophosphatase
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myo-inositol-phosphate phosphohydrolase
Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known [4].
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phosphatidylinositol 4-phosphate + H2O
phosphatidylinositol + phosphate
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alpha-D-glucose 1-phosphate
alpha-D-glucose + phosphate
IMPA1 shows intermediate activity towards alpha-D-glucose 1-phosphate, whereas IMPA2 shows significantly lower affinity
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D-myo-inositol 1-phosphate + H2O
D-myo-inositol + phosphate
IMPA1 shows strong activity towards D-myo-inositol 1-phosphate, whereas IMPA2 shows significantly lower affinity
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myo-inositol phosphate + H2O
myo-inositol + phosphate
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scyllo-inositol 1-phosphate + H2O
scyllo-inositol + phosphate
IMPA1 shows strong activity towards scyllo-inositol 1-phosphate, whereas IMPA2 shows almost no affinity
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additional information
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selctive for phosphatidylinositol 4-phosphate, no reaction with other phosphatidylinositols tested
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myo-inositol phosphate + H2O
myo-inositol + phosphate
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additional information
the enzyme shows weaker or almost no activity when Mg2+ is replaced by Ca2+, Mn2+, Na+, or K+ (2 mM each)
additional information
the enzyme shows weaker or almost no activity when Mg2+ is replaced by Ca2+, Mn2+, Na+, or K+ (2 mM each)
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Li+
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0.7
Li+
Mus musculus
IMPA1, in the presence of 2 mM MgCl2
25
Li+
Mus musculus
IMPA2, in the presence of 2 mM MgCl2
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isoform INPP5f
UniProt
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colocalizes with 5-phosphatase OCRL on endocytic membranes including vesicles formed by clathrin-mediated endocytosis, macropinosomes, and Rab5 endosomes
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physiological function
Sac2/INPP5F interacts with 5-phosphatase OCRL which is potentiated by Rab5. Rab5 activity is required to recruit Sac2/INPP5F to endosomes
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SAC2_MOUSE
1132
0
127608
Swiss-Prot
other Location (Reliability: 3 )
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homodimer
x-ray crystallography
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sitting drop vapor diffusion method, using 40% (w/v) PEG 3350, 0.2 M magnesium formate, at 22°C
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D460N
loss of activity with phosphatidylinositol 4-phosphate
D104N
IMPA2 mutant, no activity
D93N
IMPA1 mutant, no activity
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8
the activity of IMPA1 drops to 30% at pH 8.0
680880
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Q Sepharose column chromatography, Mono Q column chromatography, and Sephadex 75 gel filtration
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expressed in Escherichia coli BLR (DE3) pLysS cells
expressed in HEK-293T cells
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Ohnishi, T.; Ohba, H.; Seo, K.C.; Im, J.; Sato, Y.; Iwayama, Y.; Furuichi, T.; Chung, S.K.; Yoshikawa, T.
Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1
J. Biol. Chem.
282
637-646
2007
Mus musculus (O55023), Mus musculus (Q91UZ5)
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Singh, N.; Halliday, A.C.; Knight, M.; Lack, N.A.; Lowe, E.; Churchill, G.C.
Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens
Acta Crystallogr. Sect. F
68
1149-1152
2012
Mus musculus (O55023), Mus musculus, Homo sapiens (P29218), Homo sapiens
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Nakatsu, F.; Messa, M.; Nandez, R.; Czapla, H.; Zou, Y.; Strittmatter, S.M.; De Camilli, P.
Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway
J. Cell Biol.
209
85-95
2015
Mus musculus (Q8CDA1)
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