Information on EC 3.1.2.18 - ADP-dependent short-chain-acyl-CoA hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.2.18
-
RECOMMENDED NAME
GeneOntology No.
ADP-dependent short-chain-acyl-CoA hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + H2O = CoA + a carboxylate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-oxobutanoate degradation II
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-
SYSTEMATIC NAME
IUBMB Comments
ADP-dependent-short-chain-acyl-CoA hydrolase
Requires ADP; inhibited by NADH. Maximum activity is shown with propanoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
117698-16-5
-
124149-01-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Syrian hamster, adult female, acclimated to cold
-
-
Manually annotated by BRENDA team
male Sv/129 mice
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA + H2O
acetoacetate + CoA
show the reaction diagram
-
specific
-
-
?
acetyl-CoA + H2O
acetate + CoA
show the reaction diagram
-
low activity
-
-
?
acyl-CoA + H2O
CoA + a carboxylate
show the reaction diagram
an acyl-CoA + H2O
CoA + a carboxylate
show the reaction diagram
-
-
-
-
?
malonyl-CoA + H2O
malonate + CoA
show the reaction diagram
-
very poor substrate
-
-
?
propionyl-CoA + H2O
CoA + propionate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + H2O
CoA + a carboxylate
show the reaction diagram
an acyl-CoA + H2O
CoA + a carboxylate
show the reaction diagram
-
-
-
-
?
propionyl-CoA + H2O
CoA + propionate
show the reaction diagram
-
ensures that the propionyl-CoA level in mitochondria is not detrimentally increased
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, Km-value: 0.3 mM
K+
-
activation
Mg2+
-
activation
additional information
-
no activation by Na+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
-
inhibitory at high concentration
ATP
-
inhibitory at high concentration
beta-NADH
additional information
-
no inhibition by alpha-NADH, NADPH, NAD+, EDTA or EGTA
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
di(2-ethylhexyl)phthalate
sulfur-substituted fatty acids
-
stimulate the liver enzyme in the light-mitochondrial fraction
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additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
ADP
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pH 7.4, 25C
0.3
Ca2+
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pH 7.4, 25C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
assay at
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low expression of Acot12
Manually annotated by BRENDA team
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low expression of Acot12
Manually annotated by BRENDA team
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proximal, high expression of Acot12
Manually annotated by BRENDA team
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high expression of Acot12
Manually annotated by BRENDA team
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low expression of Acot12
Manually annotated by BRENDA team
-
low expression of Acot12
Manually annotated by BRENDA team
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low expression of Acot12
Manually annotated by BRENDA team
additional information
-
not in liver tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
low amount
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000
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at least, gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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t1/2: 1 min, in buffer with Triton X-100 but without substrates, decrease of activity in intact mitochondria, ADP stabilizes: t1/2: 1 h, substrates protect
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ADP stabilizes
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE