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Information on EC 3.1.14.1 - yeast ribonuclease
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3.1.14.1 yeast ribonucleaseSpecify your search results
Word Map
- 3.1.14.1
-
interstrand
- alpha-toxin
- exoribonuclease
- pre-trnas
- beta-toxin
-
zymocin
-
fancd2
-
karyomegalic
-
leukocidins
-
mcm5s2u
-
5-methoxycarbonylmethyl-2-thiouridine
- analysis
- nutrition
The enzyme appears in viruses and cellular organisms
Synonyms
gamma-toxin, nuclease 1, trna endonuclease, mtexo, rna-degrading enzyme, rnase trv, yeast mitochondrial degradosome, yeast ribonuclease, rna-depolymerase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
RNase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
exonucleolytic cleavage to nucleoside 3'-phosphates
-
-
-
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
-
exonucleolytic cleavage to nucleoside 3'-phosphates
exonucleolytic cleavage
Saccharomyces fragilis x Saccharomyces dobzhanski
-
exonucleolytic cleavage to nucleoside 3'-phosphates
conserved Tyr814 is involved in catalysis and is essential for enzyme Dssp1 function and mitochondrial biogenesis
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
phosphoric ester hydrolysis
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CAS REGISTRY NUMBER
COMMENTARY
9001-99-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
polyadenylic acid + H2O
3'-AMP
Saccharomyces fragilis x Saccharomyces dobzhanski
-
-
-
?
polyuridylic acid + H2O
3'-UMP
Saccharomyces fragilis x Saccharomyces dobzhanski
-
-
-
?
RNA + H2O
nucleoside 3'-phosphate + ?
-
in vitro transcribed tRNAGlu3 UUC, tRNAGIn UUG and tRNALys UUU are substrates, stable anticodon stem and the anticodon loop are the minimal requirements for cleavage, synthetic minihelix RNA corresponding to the anticodon stem loop of the natural substrate tRNAGlu3 mcm5s2UUC is cleaved at the same position as the natural substrate. In ASLGlu3 UUC, the nucleotides U34U35C36A37C38 are required for optimal gamma-toxin cleavage, whereas a purine at position 32 or a G in position 33 dramatically reduces the cleavage of the anticodon stem loop
-
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
no base specificity, preference for 3'-AMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
Saccharomyces fragilis x Saccharomyces dobzhanski
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
no base specificity, 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP, nucleotide release including 2',3'-cyclic nucloetides in decreasing order 3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP, preference for 3'-AMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
3'-AMP, 3'-GMP, 3'-UMP, 3'-CMP, 2',3' cyclic mononucleotides
?
RNA + H2O
nucleoside 3'-phosphates
-
mitochondrial mRNA and rRNA
-
?
RNA + H2O
nucleoside 3'-phosphates
-
total RNA from Schizosaccharomyces pombe
-
?
additional information
?
-
-
not: DNA, diphenylphosphate, 2',3'-cyclic nucleotides
-
-
?
additional information
?
-
-
enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex, the degradosome is not involved in tRNA processing
-
?
additional information
?
-
-
enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs
-
?
additional information
?
-
-
degradation of mitochondrial RNA to oligonucleoside 3'-phosphates after autolysis of the cells
-
-
?
additional information
?
-
-
the enzyme is responsible for RNA turnover and degradation in mitochondria, mechanism of the degradosome acting from 3' to 5', overview
-
-
?
additional information
?
-
-
degradation of mitochondrial RNA to oligonucleoside 3'-phosphates after autolysis of the cells, determination of product compositions at different conditions, overview
-
-
?
additional information
?
-
-
the enzyme is not essential for degradation of 26S and 17S rRNA
-
?
additional information
?
-
-
only slight degradation of denaturated DNA, p-nitrophenyl phosphate, bis(p-nitrophenyl)phosphate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
Saccharomyces fragilis x Saccharomyces dobzhanski
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
ribonucleic acid + H2O
3'-phosphomononucleotides
-
exonucleolytic cleavage
-
?
additional information
?
-
-
enzyme is part of the RNA degrading enzyme complex in mitochondria, the degradosome, which is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs
-
?
additional information
?
-
-
degradation of mitochondrial RNA to oligonucleoside 3'-phosphates after autolysis of the cells
-
-
?
additional information
?
-
-
the enzyme is responsible for RNA turnover and degradation in mitochondria, mechanism of the degradosome acting from 3' to 5', overview
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
KCl
Mg2+
NaCl
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Polyvinyl sulfate
Saccharomyces fragilis x Saccharomyces dobzhanski
-
70% of maximal activity with 50 microg/ml, 10% of maximal activity with 100 microg/ml
spermidine
Saccharomyces fragilis x Saccharomyces dobzhanski
-
slight inhibition: 80% of maximal activity with 0.01 M
additional information
-
tryptophan residues and disulfide bonds required for activity
-
Zn2+
-
100% inhibition by 0.1 mM ZnSO4, reversible by addition of 1 mM EDTA, sodium citrate and histidine, not by glycine, arginine, agmatine and sodium acetate even at 0.01 M
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
phenylmethanesulfonyl fluoride
-
0.1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis
protease isp6+
-
strongly dependent on, gene isp6+ encodes a protease, which is involved in RNA-degrading enzyme activity, an isp6+-deficient mutant is also devoid of RNA-degrading enzyme activity
-
Trimethylamine N-oxide
-
enhances cleavage of unmodified tRNA and anticodon stem loops by gamma-toxin-GST
EDTA
-
1 mM: inhibition of intracellular proteolytic proteins during the incubation of disrupted yeast cells for RNA hydrolysis
additional information
-
strong stimulatory effects of the mcm5 group, weak positive effect of the s2 group and a negative effect of the bacterial 5-methylaminomethyl group
-
additional information
-
enzyme is induced during nitrogen starvation
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Arthritis, Infectious
Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis.
Carcinoma
Induction of the interferon-inducible RNA-degrading enzyme, RNase L, by stress-inducing agents in the human cervical carcinoma cells.
Corneal Dystrophies, Hereditary
Polymorphism of the APEX nuclease 1 gene in keratoconus and Fuchs endothelial corneal dystrophy.
Endophthalmitis
Assessment of the role of gamma-toxin in experimental endophthalmitis using a hlg-deficient mutant of Staphylococcus aureus.
Fanconi Anemia
Case report: a 58 -year -old man with small kidneys and elevated liver enzymes.
Fanconi Anemia
Common variants in FAN1, located in 15q13.3, confer risk for schizophrenia and bipolar disorder in Han Chinese.
Fuchs' Endothelial Dystrophy
Polymorphism of the APEX nuclease 1 gene in keratoconus and Fuchs endothelial corneal dystrophy.
Huntington Disease
Genetic and Functional Analyses Point to FAN1 as the Source of Multiple Huntington Disease Modifier Effects.
Hypersensitivity
KTI11 and KTI13, Saccharomyces cerevisiae genes controlling sensitivity to G1 arrest induced by Kluyveromyces lactis zymocin.
Infections
Assessment of the role of gamma-toxin in experimental endophthalmitis using a hlg-deficient mutant of Staphylococcus aureus.
Iritis
Reactions with Antisera and Pathological Effects of Staphylococcus aureus Gamma-Toxin in the Cornea.
Keratoconus
Polymorphism of the APEX nuclease 1 gene in keratoconus and Fuchs endothelial corneal dystrophy.
Kidney Diseases
Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction.
Neoplasms
FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2.
Neoplasms
Germline Mutations in FAN1 Cause Hereditary Colorectal Cancer by Impairing DNA Repair.
Nephritis, Interstitial
Fan1 deficiency results in DNA interstrand cross-link repair defects, enhanced tissue karyomegaly, and organ dysfunction.
Nephritis, Interstitial
FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2.
Nephritis, Interstitial
Generation of a human induced pluripotent stem cell line (CMCi001-A) from a patient with karyomegalic interstitial nephritis with homozygous frameshift deletion mutation c.1985_1994del10 of the FANCD2/FANCI-Associated Nuclease 1 gene.
Starvation
Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation.
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 7
7
Saccharomyces fragilis x Saccharomyces dobzhanski
-
nuclease obtained from stationary or exponentially growing cells
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5 - 9
-
Ph 4.5: about 15% of maximum activity, pH 9.0: about 50% of maximum activity
5 - 8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 60
-
the composition of RNA degradation products varies with autolysis temperature, overview
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
Saccharomyces fragilis x Saccharomyces dobzhanski
-
released from disrupted cells
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20000 - 23000
26000 - 29000
20000 - 23000
-
207000 for RNase U4A, 21300 for RNase U4B, 22100 RNase U4C
26000 - 29000
-
29500 for RNase U4A, 26900 for RNase U4B, 20000for RNase U4C
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
enzyme is part of the RNA degrading enzyme complex, the degradosome, in mitochondria, consisting of 2 large subunits Dssp1 and Suv3p, a RNA helicase and a RNase activity, the former being essential for exoribonuclease activity of the complex
additional information
-
the enzyme is part of the mitochondrial degradosome
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Y814S
-
point mutation in Dssp1, mutant strain is respiratory incompetent with very unstable mitochondrial genomes
additional information
-
construction of a enzyme null-mutant pnu1DELTA, pnu1+ is dispensable for mitochondrial function and not essential for cell growth
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 10
5 - 10
4 - 10
-
at pH 8 stable up to at least 50°C, inactivation at higher temperatures
134106
5 - 10
-
at 40°C: inactivation rate at pH 10 markedly higher than at pH 4.1, 6.3 and 8.6
134105
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
-
remaining activity after heating at 60°C for 90 min at pH 5.4 30%, at pH 7.0 40%, at pH 9.0 50%
additional information
-
NaCl and KCl might protect from heat denaturation at 52°C
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, under toluene, pH 7.5, several weeks, little loss of activity
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant degradosome from Saccharomyces cerevisiae W303 strain derivatives
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
knowledge of amino acid sequences are useful for phylogenetic studies of several fungi
nutrition
-
possibility for reducing nucleic acid content in yeast protein used in human food
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohtaka, Y.; Uchida, K.; Sakai, T.
Purification and properties of ribonuclease from yeast
J. Biochem.
54
322-327
1963
Saccharomyces cerevisiae
brenda
Nakao, Y.; Lee, S.Y.; Halvorson, H.O.; Bock, R.M.
The ribonucleases of yeast. I. Properties and variability of ribonucleases
Biochim. Biophys. Acta
151
114-125
1968
Saccharomyces fragilis x Saccharomyces dobzhanski
brenda
Ogata, K.; Song, S.; Yamada, H.
Studies on extracellular ribonuclease from yeast. Part I. Purification and physico-chemical properties of RNase from Rhodotorula glutinis
Agric. Biol. Chem.
35
58-64
1971
Rhodotorula glutinis
-
brenda
Blank, A.; Dekker, C.A.
Ribonuclease U4 from Ustilago sphaerogena. Purification and physical properties
Biochemistry
11
3956-3962
1972
Ustilago sphaerogena
brenda
Blank, A.; Dekker, C.A.
Ribonuclease U4. Novel phosphotransferases catalyzing exonucleolytic degradation of ribonucleic acid
Biochemistry
11
3962-3970
1972
Ustilago sphaerogena
brenda
Gomes, E.; Serzedello, A.
Partial purification and some properties of a T2 ribonuclease from Aspergillus flaviceps
Microbios
87
227-237
1996
Aspergillus flavipes
-
brenda
Shetty, J.K.; Weaver, R.C.; Kinsella, J.E.
A rapid method for the isolation of ribonuclease from yeast (Saccharomyces carlsbergensis)
Biochem. J.
189
363-366
1980
Saccharomyces pastorianus
brenda
Shetty, J.K.; Weaver, R.C.; Kinsella, J.E.
Ribonuclease isolated from yeast (Saccharomyces carlsbergensis): characterization and properties
Biotechnol. Bioeng.
23
953-964
1981
Saccharomyces pastorianus
-
brenda
Inada, Y.; Watanabe, H.; Ohgi, K.; Irie, M.
Isolation, characterization, and primary structure of a base non-specific and adenylic acid preferential ribonuclease with higher specific activity from Trichoderma viride
J. Biochem.
110
896-904
1991
Trichoderma viride
brenda
Nakashima, A.; Yoshida, M.; Nakayama, K.; Kato-Furuno, A.; Ueno, M.; Ushimaru, T.; Uritani, M.
Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation
J. Biochem.
131
391-398
2002
Schizosaccharomyces pombe
brenda