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Literature summary for 3.1.14.1 extracted from

  • Gomes, E.; Serzedello, A.
    Partial purification and some properties of a T2 ribonuclease from Aspergillus flaviceps (1996), Microbios, 87, 227-237.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA 120% of maximal activity with 1 mM Aspergillus flavipes

Inhibitors

Inhibitors Comment Organism Structure
Ag+ no activity at 1 mM AgNO3 Aspergillus flavipes
Ba2+ 85% of maximal activity at 1 mM BaCl2 Aspergillus flavipes
Cd2+ 75% of maximal activity at 1 mM CdCl2 Aspergillus flavipes
Co2+ 82% of maximal activity at 1 mM CoCl2 Aspergillus flavipes
Cu2+ 63% of maximal activity at 1 mM CuSO4 Aspergillus flavipes
Fe3+ 41% of maximal activity at 1 mM FeCl3 Aspergillus flavipes
Hg2+ no activity at 1 mM HgCl2 Aspergillus flavipes
Mn2+ 85% of maximal activity at 1 mM MnSO4 Aspergillus flavipes
Zn2+ 55% of maximal activity at 1 mM ZnCl2 Aspergillus flavipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
-
Aspergillus flavipes

Organism

Organism UniProt Comment Textmining
Aspergillus flavipes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Aspergillus flavipes

Reaction

Reaction Comment Organism Reaction ID
exonucleolytic cleavage to nucleoside 3'-phosphates exonucleolytic cleavage Aspergillus flavipes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.3
-
179fold partial purification Aspergillus flavipes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleic acid + H2O exonucleolytic cleavage Aspergillus flavipes 3'-phosphomononucleotides no base specificity ?
ribonucleic acid + H2O exonucleolytic cleavage Aspergillus flavipes 3'-phosphomononucleotides preference for 3'-AMP ?

Synonyms

Synonyms Comment Organism
Afl II ribonuclease
-
Aspergillus flavipes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55 sodium acetate buffer at pH 4.0 Aspergillus flavipes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
remaining activity after heating at 60°C for 90 min at pH 5.4 30%, at pH 7.0 40%, at pH 9.0 50% Aspergillus flavipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.25 0.25 M sodium acetate buffer Aspergillus flavipes

pH Range

pH Minimum pH Maximum Comment Organism
3 9 50% of activity near pH 5.0 Aspergillus flavipes