Information on EC 3.1.1.66 - 5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene deacetylase

for references in articles please use BRENDA:EC3.1.1.66
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The expected taxonomic range for this enzyme is: Tagetes patula

EC NUMBER
COMMENTARY hide
3.1.1.66
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RECOMMENDED NAME
GeneOntology No.
5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene + H2O = 5-(3-hydroxy-4-acetoxybut-1-ynyl)-2,2'-bithiophene + acetate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
-
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SYSTEMATIC NAME
IUBMB Comments
5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene acetylhydrolase
A highly specific enzyme from Tagetes patula.
CAS REGISTRY NUMBER
COMMENTARY hide
95990-32-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-diacetoxybutynylbithiophene + H2O
3-hydroxy-4-acetoxybutynylbithiophene + acetate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,4-diacetoxybutynylbithiophene + H2O
3-hydroxy-4-acetoxybutynylbithiophene + acetate
show the reaction diagram
-
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest activity
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
- 20°C, storage in ethylene glycol monomethylether
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE