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Information on EC 3.1.1.17 - gluconolactonase and Organism(s) Zymomonas mobilis and UniProt Accession Q01578

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.17 gluconolactonase
IUBMB Comments
Acts on a wide range of hexose-1,5-lactones. The hydrolysis of L-gulono-1,5-lactone was previously listed separately.
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Zymomonas mobilis
UNIPROT: Q01578
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Word Map
The taxonomic range for the selected organisms is: Zymomonas mobilis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
regucalcin, lactonase, smp30, senescence marker protein-30, smp30/gnl, human paraoxonase 1, senescence marker protein 30, gluconolactonase, smp-30, aldonolactonase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldonolactonase
-
-
-
-
D-glucono-delta-lacton-hydrolase
-
-
-
-
D-glucono-delta-lactone lactonohydrolase
-
-
-
-
GL
-
-
-
-
glucono-delta-lactonase
gulonolactonase
-
-
-
-
lactonase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glucono-1,5-lactone lactonohydrolase
Acts on a wide range of hexose-1,5-lactones. The hydrolysis of L-gulono-1,5-lactone was previously listed separately.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-44-6
EC 3.1.1.18
9012-73-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucono-1,5-lactone + H2O
D-gluconate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucono-1,5-lactone + H2O
D-gluconate
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
-
10 mM, 20% inhibition
metal-complexing agents
-
activity is restored by excess of Ca2+, Mn2+ or Zn2+
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
3 * 30000 or 4 * 44000, SDS-PAGE
32000
-
2 * 32000, SDS-PAGE
44000
-
3 * 30000 or 4 * 44000, SDS-PAGE
63000
-
gel filtration
90000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
3 * 30000 or 4 * 44000, SDS-PAGE
dimer
-
2 * 32000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
the enzyme is useful in the enzymatic bioconversion of fructose and lactose to gluconic acid and sorbitol, respectively, by the enzyme's glucose-fructose oxidoreductase and gluconolactonase activities, method development and evaluation for separation of reaction product mixtures containing lactobionic acid, sorbitol, lactose and fructose by HPLC, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli JM101
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zachariou, M.; Scopes, R.K.
Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production
J. Bacteriol.
167
863-869
1986
Zymomonas mobilis
Manually annotated by BRENDA team
Nidetzky, B.; Furlinger, M.; Gollhofer, D.; Haug, I.; Haltrich, D.; Kulbe, K.D.
Simultaneous enzymatic synthesis of gluconic acid and sorbitol. Production, purification, and application of glucose-fructose oxidoreductase and gluconolactonase
Appl. Biochem. Biotechnol.
63-65
173-188
1997
Delftia acidovorans, Pseudomonas aeruginosa, Pseudomonas chlororaphis, Pseudomonas fluorescens, Rhodotorula mucilaginosa, Zymomonas mobilis
Manually annotated by BRENDA team
Silveira, M.M.; Wisbeck, E.; Lemmel, C.; Erzinger, G.; Lopes da Costa, J.P.; Bertasso, M.; Jonas, R.
Bioconversion of glucose and fructose to sorbitol and gluconic acid by untreated cells of Zymomonas mobilis
J. Biotechnol.
75
99-103
1999
Zymomonas mobilis
Manually annotated by BRENDA team
Kanagasundaram, V.; Scopes, R.
Isolation and characterization of the gene encoding gluconolactonase from Zymomonas mobilis
Biochim. Biophys. Acta
1171
198-200
1992
Zymomonas mobilis
Manually annotated by BRENDA team
Wisbeck, E.; Silveira, M.M.; Ninow, J.; Jonas, R.
Evaluation of the flocculent strain Zymomonas mobilis Z1-81 for the production of sorbitol and gluconic acid
J. Basic Microbiol.
37
445-449
1997
Zymomonas mobilis, Zymomonas mobilis Z1-81
-
Manually annotated by BRENDA team
Pedruzzi, I.; Malvessi, E.; Mata, V.G.; Silva, E.A.; Silveira, M.M.; Rodrigues, A.E.
Quantification of lactobionic acid and sorbitol from enzymatic reaction of fructose and lactose by high-performance liquid chromatography
J. Chromatogr. A
1145
128-132
2007
Zymomonas mobilis
Manually annotated by BRENDA team