Ligand D-gluconate

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Basic Ligand Information

Molecular Structure
Picture of D-gluconate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6H12O7
D-gluconate
RGHNJXZEOKUKBD-SQOUGZDYSA-N
Synonyms:
aldonic acid, D-gluconic acid, gluconate, gluconic acid


Show all pahtways known for Show all BRENDA pathways known for D-gluconate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (16 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
gluconate + NAD(P)H = D-glucose + NAD(P)+
-
show the reaction diagram
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O
-

In Vivo Product in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
D-glucose + NADP+ = D-gluconate + NADPH + H+
-
-
show the reaction diagram
D-glucono-1,5-lactone + H2O = D-gluconate
-

Substrate in Enzyme-catalyzed Reactions (49 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
D-gluconate + NADH + H+ = ?
-
show the reaction diagram
D-gluconate + NAD+ = ?
-
show the reaction diagram
D-gluconate + NADP+ = ? + NADPH
-
show the reaction diagram
gluconate + NAD(P)H = D-glucose + NAD(P)+
-
show the reaction diagram
D-gluconic acid + NAD+ = ?
-
show the reaction diagram
gluconate + NAD+ = ? + NADH
-
show the reaction diagram
D-gluconate + 2,6-dichlorophenolindophenol = ?
-
show the reaction diagram
D-gluconate + acceptor = 5-keto-D-gluconate + reduced acceptor
-
show the reaction diagram
D-gluconate + acceptor = 2-oxo-gluconate + reduced acceptor
-
show the reaction diagram
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O
-
show the reaction diagram
D-gluconate = ?
-
show the reaction diagram
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O
-

Product in Enzyme-catalyzed Reactions (38 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
2-dehydro-D-gluconate + NADPH + H+ = D-gluconate + NADP+
-
-
show the reaction diagram
2-ketogluconate + NAD(P)H = D-gluconate + NAD(P)+
-
-
show the reaction diagram
D-glucose + NAD(P)+ = D-gluconate + NAD(P)H
-
-
show the reaction diagram
2-dehydro-D-gluconate + NADPH + H+ = D-gluconate + NADP+
-
show the reaction diagram
D-glucose + 2,6-dichlorophenolindophenol = D-gluconic acid + reduced 2,6-dichlorophenolindophenol
-
show the reaction diagram
cellodextrin + O2 = aldonic acid + H2O2
-
-
show the reaction diagram
D-glucose + NAD+ + H2O = D-gluconate + NADH
-
-
show the reaction diagram
delta-D-gluconolactone + H2O = D-gluconate
-
-
show the reaction diagram
6-phospho-D-gluconate + H2O = D-gluconate + phosphate
-
-
show the reaction diagram
6-phospho-D-gluconate + H2O = D-gluconate + phosphate
-
-
show the reaction diagram
6-phosphogluconate + H2O = gluconate + phosphate
-
-
show the reaction diagram
D-gluconate 6-phosphate + H2O = D-gluconate + phosphate
-
-
show the reaction diagram
6-phosphogluconate + H2O = gluconate + phosphate
-

Activator in Enzyme-catalyzed Reactions (5 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (8 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
non-competitive inhibitor to D-glucose and competitive inhibitor to N-methylphenazonium methyl sulfate
-
slight inhibition
-
binds to an enzyme-ADP complex forming a dead-end complex
-
17% of initial activity at 0.2 M
1 mM, 87% inhibition
-
10 mM, 100% inhibition
-
32% inhibition at 1 mM
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3D Structure of Enzyme-Ligand-Complex (PDB) (5 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (14 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
at pH 7.9 and 25°C
0.98
-
pH 8, temperature not specified in the publication
2.6
-
mutant Q190A, pH 6.5, 30°C
24
-
pH 6.0-6.5, 30°C
pH 6.5, 30°C
wild-type, pH 6.5, 30°C
130
-
pH 7.2, temperature not specified in the publication
9.3
-
pH 6.0, 70°C
10.4
-
pH 7, 50°C, enzyme activated by 2-mercaptoethanol
1.19
-
pH 7, 50°C, non-activated enzyme
0.4
-

KM Value (43 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
at pH 7.9 and 25°C
12
-
-
4.4
-
pH 6, 25°C
0.63
-
cofactor NAD+, pH 8.5
19
-
pH 8, temperature not specified in the publication
8.4
-
pH 5.5, 30°C
161
-
pH 4
2.9
-
pH 5.0
3.2
-
pH 8.5, 38°C
0.98
-
pH 6.0-6.5, 30°C
pH 6.5, 30°C
mutant Q190A, pH 6.5, 30°C
41
-
wild-type, pH 6.5, 30°C
1.7
-
30°C, pH 7.6
1.74
-
pH 7.2, temperature not specified in the publication
0.106
-
25°C, pH 7
0.042
-
25°C, pH 8, gene gntU
0.212
-
pH 6.0, 70°C
1.57
-
pH 6.2, 60°C
2.5
-
pH 7.5, 42°C
3
-
at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2
0.4
-
-
2.42
-
pH 7, 50°C
7.8
-

Ki Value (2 results)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
sucrose concentration 1.2-4.9 mM
6.3
-
sucrose concentration 12.2-97.4 mM
7.5
-

References & Links

Links to other databases for D-gluconate

ChEBI
PubChem
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PubChem