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IUBMB Comments The enzyme, characterized from Pseudomonas strains, is involved in degradation of short chain alkyl methyl ketones.
The expected taxonomic range for this enzyme is: Pseudomonas
Synonyms
estZ ,
mekB ,
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estZ
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mekB
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ethyl acetate + H2O = acetate + ethanol
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-
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-
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ethyl acetate acetohydrolase
The enzyme, characterized from Pseudomonas strains, is involved in degradation of short chain alkyl methyl ketones.
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4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
?
alpha-naphthyl caproate + H2O
alpha-naphthol + caproate
-
-
-
?
alpha-naphthyl propionate + H2O
alpha-naphthol + propionate
-
-
-
?
ethyl acetate + H2O
acetate + ethanol
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
methyl acetate + H2O
methanol + acetate
propyl acetate + H2O
propanol + acetate
vinyl acetate + H2O
ethenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
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-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
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-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
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-
-
?
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
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-
-
?
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
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-
-
?
ethyl acetate + H2O
ethanol + acetate
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-
-
?
ethyl acetate + H2O
ethanol + acetate
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-
-
?
methyl acetate + H2O
methanol + acetate
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-
-
?
methyl acetate + H2O
methanol + acetate
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-
-
?
propyl acetate + H2O
propanol + acetate
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-
-
?
propyl acetate + H2O
propanol + acetate
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-
-
?
propyl acetate + H2O
propanol + acetate
-
-
-
?
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ethyl acetate + H2O
acetate + ethanol
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-
-
?
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diethyldicarbonate
almost complete inhibition
phenylmethylsulfonyl fluoride
almost complete inhibition
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0.054
4-nitrophenyl acetate
37°C, pH 7.5
0.018
Alpha-naphthyl acetate
37°C, pH 7.5
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64
4-nitrophenyl acetate
37°C, pH 7.5
29
Alpha-naphthyl acetate
37°C, pH 7.5
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1200
4-nitrophenyl acetate
37°C, pH 7.5
1600
Alpha-naphthyl acetate
37°C, pH 7.5
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11
substrate 4-nitrophenylacetate, pH 7.5, 37°C
15
substrate alpha-naphthyl caproate, pH 7.5, 37°C
217
substrate 4-nitrophenyl acetate, pH 7.0, 30°C
230
substrate ethyl acetate, pH 7.0, 30°C
288
substrate vinyl acetate, pH 7.0, 30°C
3.4
substrate 4-nitrophenyl butanoate, pH 7.0, 30°C
33
substrate alpha-naphthyl propionate, pH 7.5, 37°C
42
substrate alpha-naphthyl acetate, pH 7.5, 37°C
77
substrate propyl acetate, pH 7.0, 30°C
78
substrate methyl acetate, pH 7.0, 30°C
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UniProt
brenda
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UniProt
brenda
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UniProt
brenda
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physiological function
enzyme belongs to family IV of lipolytic enzymes and contains the conserved catalytic triad of serine, aspartic acid, and histidine
physiological function
strain Pseudomonas veronii MEK700 is able to grow on 2-butanone and 2-hexanol. Inactivation of monooxygenase MekA and MekB abolishes growth . All three genes MekA, MekB and transcriptional activator MekR are required for growth of Pseudomonas veronii MEK700 on 2-butanone and 2-hexanol
physiological function
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strain Pseudomonas veronii MEK700 is able to grow on 2-butanone and 2-hexanol. Inactivation of monooxygenase MekA and MekB abolishes growth . All three genes MekA, MekB and transcriptional activator MekR are required for growth of Pseudomonas veronii MEK700 on 2-butanone and 2-hexanol
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Q0MRG5_9PSED
349
0
38289
TrEMBL
-
Q8KQK1_PSEPU
318
0
34320
TrEMBL
-
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monomer
1 * 36000, SDS-PAGE
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expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
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analysis
method to rapidly screen bacterial colonies for the presence of esterases which hydrolyze ethyl acetate based on pH change
synthesis
expression of EstZ in Escherichia coli KO11 reduces the concentration of ethyl acetate in fermentation broth (4.8xa0% ethanol) to less than 20 mg/l
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Hasona, A.; York, S.; Yomano, L.; Ingram, L.; Shanmugam, K.
Decreasing the level of ethyl acetate in ethanolic fermentation broths of Escherichia coli KO11 by expression of Pseudomonas putida estZ esterase
Appl. Environ. Microbiol.
68
2651-2659
2002
Pseudomonas putida (Q8KQK1)
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brenda
Onaca, C.; Kieninger, M.; Engesser, K.; Altenbuchner, J.
Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700
J. Bacteriol.
189
3759-3767
2007
Pseudomonas veronii (Q0MRG5), Pseudomonas veronii MEK700 (Q0MRG5)
brenda
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Enzyme Nomenclature
3.1.1.113 ethyl acetate hydrolase
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