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Enzyme Nomenclature
Information on EC 3.1.1.113 - ethyl acetate hydrolase
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3.1.1.113 ethyl acetate hydrolaseIUBMB Comments
The enzyme, characterized from Pseudomonas strains, is involved in degradation of short chain alkyl methyl ketones.
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The expected taxonomic range for this enzyme is: Pseudomonas
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
estZ
mekB
estZ
-
-
-
-
mekB
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ethyl acetate + H2O = acetate + ethanol
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ethyl acetate acetohydrolase
The enzyme, characterized from Pseudomonas strains, is involved in degradation of short chain alkyl methyl ketones.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-naphthyl propionate + H2O
alpha-naphthol + propionate
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-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
enzyme belongs to family IV of lipolytic enzymes and contains the conserved catalytic triad of serine, aspartic acid, and histidine
physiological function
strain Pseudomonas veronii MEK700 is able to grow on 2-butanone and 2-hexanol. Inactivation of monooxygenase MekA and MekB abolishes growth . All three genes MekA, MekB and transcriptional activator MekR are required for growth of Pseudomonas veronii MEK700 on 2-butanone and 2-hexanol
physiological function
Pseudomonas veronii MEK700
-
strain Pseudomonas veronii MEK700 is able to grow on 2-butanone and 2-hexanol. Inactivation of monooxygenase MekA and MekB abolishes growth . All three genes MekA, MekB and transcriptional activator MekR are required for growth of Pseudomonas veronii MEK700 on 2-butanone and 2-hexanol
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
method to rapidly screen bacterial colonies for the presence of esterases which hydrolyze ethyl acetate based on pH change
synthesis
expression of EstZ in Escherichia coli KO11 reduces the concentration of ethyl acetate in fermentation broth (4.8xa0% ethanol) to less than 20 mg/l
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hasona, A.; York, S.; Yomano, L.; Ingram, L.; Shanmugam, K.
Decreasing the level of ethyl acetate in ethanolic fermentation broths of Escherichia coli KO11 by expression of Pseudomonas putida estZ esterase
Appl. Environ. Microbiol.
68
2651-2659
2002
Pseudomonas putida (Q8KQK1)
-
brenda
Onaca, C.; Kieninger, M.; Engesser, K.; Altenbuchner, J.
Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700
J. Bacteriol.
189
3759-3767
2007
Pseudomonas veronii (Q0MRG5), Pseudomonas veronii MEK700 (Q0MRG5)
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