The enzyme, which has been described from mammals, is specific for phosphatidylserine and 2-lysophosphatidylserine, and does not act on phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid or phosphatidylinositol.
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SYSTEMATIC NAME
IUBMB Comments
3-sn-phosphatidyl-L-serine sn-1 acylhydrolase
The enzyme, which has been described from mammals, is specific for phosphatidylserine and 2-lysophosphatidylserine, and does not act on phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid or phosphatidylinositol.
an alternative splicing 376-amino acid product lacks two-thirds of the C-terminal domain of PS-PLA1 hydrolyzes exclusively lyso-phosphatidylserine but not phosphatidylserine appreciably. Phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, and their lyso derivatives are not hydrolyzed at all by the variant
an alternative splicing 376-amino acid product lacks two-thirds of the C-terminal domain of PS-PLA1 hydrolyzes exclusively lyso-phosphatidylserine but not phosphatidylserine appreciably. Phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, and their lyso derivatives are not hydrolyzed at all by the variant
an alternative splicing 376-amino acid product lacks two-thirds of the C-terminal domain of PS-PLA1 hydrolyzes exclusively lyso-phosphatidylserine but not phosphatidylserine appreciably. Phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, and their lyso derivatives are not hydrolyzed at all by the variant
an alternative splicing 376-amino acid product lacks two-thirds of the C-terminal domain of PS-PLA1 hydrolyzes exclusively lyso-phosphatidylserine but not phosphatidylserine appreciably. Phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, and their lyso derivatives are not hydrolyzed at all by the variant
An alternative splicing form of phosphatidylserine-specific phospholipase A1 that exhibits lysophosphatidylserine-specific lysophospholipase activity in humans.
transcripts are detected in various human tissues, including skeletal muscle, kidney, small intestine, spleen, and testis. The amount of the splicing variant in these tissues is about 10 to 20% that of the wild-type
transcripts are detected in various human tissues, including skeletal muscle, kidney, small intestine, spleen, and testis. The amount of the splicing variant in these tissues is about 10 to 20% that of the wild-type
PLA1A is upregulated by hepatitis C virus HCV infection, and PLA1A knockdown significantly reduces J399EM (genotype 2a) HCV propagation at the assembly step but not the entry, RNA replication, and protein translation steps of the life cycle. PLA1A has a role in the interaction of viral proteins NS2-E2 and NS2-NS5A. PLA1A stabilizes the nonstructural proteins NS2/NS5A dotted structure during infection
review on extracellular PLA1s including phosphatidylserine-specific PLA1, membrane-associated phosphatidic acid-selective mPA-PLA1alpha and mPA-PLA1beta. The tertiary structures of lipases show two surface loops, the lid and the beta9 loop. The lid and the beta9 loop cover the active site in its closed conformation. Phosphatidylserine-specific PLA1, membrane-associated phosphatidic acid-selective mPA-PLA1alpha and mPA-PLA1beta have short lids and short loops. They specifically hydrolyze phospatidylserine and phosphatidic acid, respectively, producing their corresponding lysophospholipids
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
membrane-associated mPA-PLA1 is insoluble in solubilization by 1% Triton X-100 and is detected in Triton X-100-insoluble buoyant fractions of sucrose gradients
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
corticosteroids, prednisolone, 6alpha-methylprednisolone, dexamethasone, and beclomethasone inhibite PS-PLA1 expression with half-maximal inhibitory concentrations less than 3.0 nM. Methotrexate, cyclosporine A, tacrolimus, 6-mercaptopurine, and mycophenoic acid show either a weak or moderate inhibition
expression is enhanced in THP-1 derived macrophages stimulated with lipopolysaccharide. Other toll-like receptor ligands (TLR2, 3, 5, 7, and 9) do not show a significant induction of PS-PLA1 mRNA
immunoassay for determination of enzyme in blood serum samples. The mean standard deviation of the serum PS-PLA1 antigen concentration in 191 healthy subjects is 33.8 +- 16.6 microg/l. The concentration is significantly higher among men (13.8-80.6 microg/l) than among women (12.1-68.8 microg/l)
blood serum PS-PLA1 level is significantly higher in systemic lupus erythematosus (SLE) patients than in healthy controls, active rheumatoid arthritis and Sjoegren's syndrome patients. Although PS-PLA1 is significantly elevated in systemic sclerosis and Sjoegren's syndrome patients compared with healthy controls, PS-PLA1 is significantly higher in untreated SLE patients than in treated SLE patients and disease control patients. A cut-off value of 18.2 ng/ml distinguishes untreated SLE from disease control, with sensitivity and specificity of 71.4% and 57.5%, respectively. PS-PLA1 is significantly correlated with SLE Disease Activity Index (SLEDAI) and immunoglobulin G (IgG), and inversely correlated with white blood cell counts, lymphocyte counts, total complement hemolytic activity (CH50), complements C3, and C4 in SLE patients overall
during infection with hepatitis C virus, PLA1A plays an important role in bridging the membrane-associated NS2-E2 complex and the NS5A-associated replication complex via its interaction with E2, NS2, and NS5A
An alternative splicing form of phosphatidylserine-specific phospholipase A1 that exhibits lysophosphatidylserine-specific lysophospholipase activity in humans