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Information on EC 2.8.2.11 - galactosylceramide sulfotransferase and Organism(s) Homo sapiens and UniProt Accession Q99999

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.11 galactosylceramide sulfotransferase
IUBMB Comments
Also acts on lactosylceramide.
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This record set is specific for:
Homo sapiens
UNIPROT: Q99999
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
cerebroside sulfotransferase, gal3st1, glycolipid sulfotransferase, galactosylceramide sulfotransferase, galactolipid sulfotransferase, galactocerebroside sulfotransferase, galcer sulfotransferase, galactosylceramide 3'-sulfotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
galactosylceramide 3'-sulfotransferase
-
3'-phosphoadenosine-5'-phosphosulfate-cerebroside sulfotransferase
-
-
-
-
cerebroside sulfotransferase
galactocerebroside sulfotransferase
-
-
-
-
galactolipid sulfotransferase
-
-
-
-
galactosylceramide sulfotransferase
-
-
-
-
glycolipid sulfotransferase
-
-
-
-
glycosphingolipid sulfotransferase
-
-
-
-
GSase
-
-
-
-
sulfotransferase, galactocerebroside
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:galactosylceramide 3'-sulfotransferase
Also acts on lactosylceramide.
CAS REGISTRY NUMBER
COMMENTARY hide
9081-06-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + a galactosylceramide
adenosine 3',5'-bisphosphate + a galactosylceramidesulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + galactosylceramide
adenosine 3',5'-bisphosphate + galactosylceramide 3'-sulfate
show the reaction diagram
-
-
-
?
galactocerebroside + 3'-phosphoadenylylsulfate
sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + cerebroside
adenosine 3',5'-bisphosphate + cerebroside 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + psychosine
adenosine 3',5'-bisphosphate + psychosine 3-sulfate
show the reaction diagram
-
-
-
-
?
galactosyl 1-alkyl-2-acyl-sn-glycerol + 3'-phosphoadenylylsulfate
galactosyl 1-alkyl-2-acyl-sn-glycerol sulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
-
?
galactosyl diacylglycerol + 3'-phosphoadenylylsulfate
galactosyl diacylglycerol sulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
-
?
galactosylceramide + 3'-phosphoadenylylsulfate
galactosylceramide sulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
lactosylceramide + 3'-phosphoadenylylsulfate
lactosylceramide sulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + a galactosylceramide
adenosine 3',5'-bisphosphate + a galactosylceramidesulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + galactosylceramide
adenosine 3',5'-bisphosphate + galactosylceramide 3'-sulfate
show the reaction diagram
-
-
-
?
galactocerebroside + 3'-phosphoadenylylsulfate
sulfogalactoglycerolipid + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine diphosphopyridoxal
-
followed by reduction with NaBH4, addition of adenosine 3’-phosphate 5’-phosphosulfate results in protection against the inactivation
brefeldin A
-
-
Congo red
-
uncompetitive inhibition
NaCl
-
inhibition at concentration above 0.1 M
pyridoxal 5'phosphate
-
followed by reduction with NaBH4, irreversible inactivation, addition of adenosine 3’-phosphate 5’-phosphosulfate results in protection against the inactivation
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NaCl
-
activation at concentration up to 0.1 M
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025 - 0.074
3'-phosphoadenylylsulfate
0.0603
cerebroside
-
at pH 7.1 and 37°C
0.027
galactosylceramide
-
pH 6.5, 37°C
0.103
psychosine
-
at pH 7.1 and 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.17
adenosine diphosphopyridoxal
-
pH 7.0, 4°C
0.287
pyridoxal 5'phosphate
-
pH 7.0, 4°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0381
Congo red
Homo sapiens
-
at pH 7.1 and 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
renal cell carcinoma cell line, SMKT-R3 cells
Manually annotated by BRENDA team
reduced expression of the enzyme in the gastric mucosa of chronic gastritis according to the severety of gastric inflammation
Manually annotated by BRENDA team
from patients with chronic gastritis
Manually annotated by BRENDA team
-
renal cell carcinoma cell line, SMKT-R3 cells
Manually annotated by BRENDA team
-
the glycosphingolipid beta-galactosylceramide-3-O-sulfate is present in the secretory granules of the insulin producing beta-cells
Manually annotated by BRENDA team
-
S16 immortalized Schwann cell line
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
G3ST1_HUMAN
423
1
48764
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
-
1 * 54000 SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 54000 SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
rs2267161
-
single nucleotide polymorphism located in an exon in the gene encoding CST, heterozygosity confers increased risk of type 2 diabetes
rs42929
-
single nucleotide polymorphism located in an intron in the gene encoding CST
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 20% glycerol, stable for 3 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
when the cDNA is introduced into a eukaryotic expression vector and transfected into COS-1 cells, the enzyme activity expressed exceeds that in controls by 8-16fold
expressed in CHO cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
heterozygosity at SNP rs2267161 in the gene encoding cerebroside sulfotransferase confers increased risk of type 2 diabetes, females with the CC allele show lower insulin resistance
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mitsuyama, T.; Gasa, S.; Nojima, T.; Taniguchi, N.; Makita, A.
Purification and properties of galactosylceramide sulfatase activator from human liver
J. Biochem.
98
605-613
1985
Homo sapiens
Manually annotated by BRENDA team
Kobayashi, T.; Honke, K.; Gasa, S.; Miyazaki, T.; Tajima, H.; Matsumoto, K.; Nakamura, T.; Makita, A.
Hepatocyte growth factor elevates the activity levels of glycolipid sulfotransferases in renal cell carcinoma cells
Eur. J. Biochem.
219
407-413
1994
Homo sapiens
Manually annotated by BRENDA team
Farrer, R.G.; Warden, M.P.; Quarles, R.H.
Effects of brefeldin A on galactosphingolipid synthesis in an immortalized Schwann cell line: evidence for different intracellular locations of galactosylceramide sulfotransferase and ceramide galactosyltransferase activities
J. Neurochem.
65
1865-1873
1995
Homo sapiens
Manually annotated by BRENDA team
Kamio, K.; Honke, K.; Makita, A.
Pyridoxal 5'-phosphate binds to a lysine residue in the adenosine 3'-phosphate 5'-phosphosulfate recognition site of glycolipid sulfotransferase from human renal cancer cells
Glycoconj. J.
12
762-766
1995
Homo sapiens
Manually annotated by BRENDA team
Honke, K.; Yamane, M.; Ishii, A.; Kobayashi, T.; Makita, A.
Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells
J. Biochem.
119
421-427
1996
Homo sapiens
Manually annotated by BRENDA team
Honke, K.; Tsuda, M.; Hirahara, Y.; Ishii, A.; Makita, A.; Wada, Y.
Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
J. Biol. Chem.
272
4864-4868
1997
Homo sapiens (Q99999), Homo sapiens
Manually annotated by BRENDA team
Hayashi, A.; Nakashima, K.; Yamagishi, K.; Hoshi, T.; Suzuki, A.; Baba, H.
Localization of annexin II in the paranodal regions and Schmidt-Lanterman incisures in the peripheral nervous system
Glia
55
1044-1052
2007
Homo sapiens (Q99999)
Manually annotated by BRENDA team
Roeske-Nielsen, A.; Buschard, K.; Manson, J.; Rastam, L.; Lindblad, U.
A variation in the cerebroside sulfotransferase gene is linked to exercise-modified insulin resistance and to type 2 diabetes
Exp. Diabetes Res.
2009
429593
2009
Homo sapiens
Manually annotated by BRENDA team
Li, W.; Zech, I.; Gieselmann, V.; Mueller, C.E.
A capillary electrophoresis method with dynamic pH junction stacking for the monitoring of cerebroside sulfotransferase
J. Chromatogr. A
1407
222-227
2015
Homo sapiens
Manually annotated by BRENDA team