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Information on EC 2.7.8.34 - CDP-L-myo-inositol myo-inositolphosphotransferase and Organism(s) Archaeoglobus fulgidus and UniProt Accession O29976

for references in articles please use BRENDA:EC2.7.8.34
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IUBMB Comments
In many organisms this activity is catalysed by a bifunctional enzyme. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain of the bifunctional EC 2.7.7.74/EC 2.7.8.34 (CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase) uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-1L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors. The enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3-phosphate, a widespread organic solute in microorganisms adapted to hot environments.
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This record set is specific for:
Archaeoglobus fulgidus
UNIPROT: O29976
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The taxonomic range for the selected organisms is: Archaeoglobus fulgidus
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
dipps, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CDP-inositol:inositol-1-phosphate transferase
bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS)
DIPPS
bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (IPCT/DIPPS)
CTP:inositol-1-phosphate cytidylyltransferase
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bifunctional enzyme
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
CDP-1L-myo-inositol:1L-myo-inositol 1-phosphate myo-inositolphosphotransferase
In many organisms this activity is catalysed by a bifunctional enzyme. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain of the bifunctional EC 2.7.7.74/EC 2.7.8.34 (CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase) uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-1L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors. The enzyme is involved in biosynthesis of bis(1L-myo-inositol) 1,3-phosphate, a widespread organic solute in microorganisms adapted to hot environments.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
show the reaction diagram
CDP-glycerol + 1L-myo-inositol 1-phosphate
CMP + 1L-(1X-glyceryl)-myo-inositol phosphate 3-phosphate
show the reaction diagram
bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase. The cytidylyltransferase is absolutely specific for CTP and 1L-myo-inositol 1-phosphate. The di-myo-inositol-1,3'-phosphate-1'-phosphate synthase domain uses only 1L-myo-inositol 1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-L-myo-inositol and CDP-D-myo-inositol, are recognized as alcohol donors
-
-
?
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in synthesis of di-myo-inositol phosphate. Archaeoglobus fulgidus accumulates di-myo-inositol phosphate and diglycerol phosphate in response to heat and osmotic stresses, respectively, and the level of glycero-phospho-myo-inositol increases primarily when the two stresses are combined
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apo enzyme, sitting drop vapor diffusion method, using 1.2-1.4 M trisodium citrate dihydrate, 100 mM HEPES pH 6.8-7.2 and 10 mM MgCl2
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-Trap column chromatography and Superdex G75 gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli BL21(DE3)pLyS cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Borges, N.; Goncalves, L.G.; Rodrigues, M.V.; Siopa, F.; Ventura, R.; Maycock, C.; Lamosa, P.; Santos, H.
Biosynthetic pathways of inositol and glycerol phosphodiesters used by the hyperthermophile Archaeoglobus fulgidus in stress adaptation
J. Bacteriol.
188
8128-8135
2006
Archaeoglobus fulgidus (O29976)
Manually annotated by BRENDA team
Rodrigues, M.V.; Borges, N.; Henriques, M.; Lamosa, P.; Ventura, R.; Fernandes, C.; Empadinhas, N.; Maycock, C.; da Costa, M.S.; Santos, H.
Bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase, the key enzyme for di-myo-inositol-phosphate synthesis in several (hyper)thermophiles
J. Bacteriol.
189
5405-5412
2007
Aquifex aeolicus (O67379), Archaeoglobus fulgidus (O29976), Pyrococcus furiosus (Q8U1Z6), Thermococcus kodakarensis (Q5JDA9)
Manually annotated by BRENDA team
Brito, J.A.; Borges, N.; Santos, H.; Archer, M.
Production, crystallization and preliminary X-ray analysis of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus
Acta Crystallogr. Sect. F
66
1463-1465
2010
Archaeoglobus fulgidus, Archaeoglobus fulgidus AF0263
Manually annotated by BRENDA team