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Information on EC 2.7.2.1 - acetate kinase and Organism(s) Methanosarcina mazei and UniProt Accession P0CW05

for references in articles please use BRENDA:EC2.7.2.1
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EC Tree
IUBMB Comments
Requires Mg2+ for activity. While purified enzyme from Escherichia coli is specific for acetate , others have found that the enzyme can also use propanoate as a substrate, but more slowly . Acetate can be converted into the key metabolic intermediate acetyl-CoA by coupling acetate kinase with EC 2.3.1.8, phosphate acetyltransferase. Both this enzyme and EC 2.7.2.15, propionate kinase, play important roles in the production of propanoate .
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This record set is specific for:
Methanosarcina mazei
UNIPROT: P0CW05
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The taxonomic range for the selected organisms is: Methanosarcina mazei
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
acetate kinase, ack, acetokinase, acka2, acka1, ackase, atp-ecoak, urkinase, acetic kinase, atp-specific ak, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MM_0495
locus name
acetate kinase (phosphorylating)
-
-
-
-
acetic kinase
-
-
-
-
acetokinase
-
-
-
-
AK
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:acetate phosphotransferase
Requires Mg2+ for activity. While purified enzyme from Escherichia coli is specific for acetate [4], others have found that the enzyme can also use propanoate as a substrate, but more slowly [7]. Acetate can be converted into the key metabolic intermediate acetyl-CoA by coupling acetate kinase with EC 2.3.1.8, phosphate acetyltransferase. Both this enzyme and EC 2.7.2.15, propionate kinase, play important roles in the production of propanoate [9].
CAS REGISTRY NUMBER
COMMENTARY hide
9027-42-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + acetate
ADP + acetyl phosphate
show the reaction diagram
-
-
-
?
ATP + acetate
ADP + acetyl phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + acetate
ADP + acetyl phosphate
show the reaction diagram
-
-
-
?
ATP + acetate
ADP + acetyl phosphate
show the reaction diagram
acetate kinase is a key enzymes of acetate metabolism of anaerobes
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 2
acetate
pH 7.0, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
251
isolated enzyme, pH 7.0, temperature not specified in the publication
7
cell lysate, pH 7.0, temperature not specified in the publication
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 43
20°C: 75% of maximal activity, 43°C: 45% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
calculated from sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
at a concentration of 2 mM acetate, the acetate kinase activity in cell extract of Methanosarcina mazei is not limiting the methane formation rate
physiological function
acetate kinase is a key enzyme of acetate metabolism of anaerobes
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44500
x * 44500, calculated from sequence
45000
x * 45000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
functionally expressed of the His6-tagged enzyme in Escherichia coli JM109
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ingram-Smith, C.; Martin, S.R.; Smith, K.S.
Acetate kinase: not just a bacterial enzyme
Trends Microbiol.
14
249-253
2006
Mesoplasma florum, Histoplasma capsulatum, Cupriavidus necator, Aspergillus fumigatus, Aspergillus nidulans, Bacillus subtilis, Borreliella burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Phanerodontia chrysosporium, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Streptococcus pneumoniae, Entamoeba histolytica, Enterococcus faecalis, Salmonella enterica, Fusarium graminearum, Helicobacter pylori, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Pyricularia grisea, Methanosarcina mazei, Methanosarcina thermophila, Staphylococcus aureus, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Phytophthora sojae, Sclerotinia sclerotiorum, Trichoderma reesei, Ustilago maydis, Methanosarcina acetivorans, Parastagonospora nodorum, Uncinocarpus reesii, Phytophthora ramorum
Manually annotated by BRENDA team
Tonouchi, A.; Nishizaki, Y.; Tohyama, H.; Takeda, K.
Cloning of a gene encoding acetate kinase from Methanosarcina mazei 2-P isolated from a Japanese paddy field soil
Curr. Microbiol.
45
390-393
2002
Methanosarcina mazei (P0CW04), Methanosarcina mazei 2-P (P0CW04), Methanosarcina mazei 2-P
Manually annotated by BRENDA team
Welte, C.; Krninger, L.; Deppenmeier, U.
Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei
FEMS Microbiol. Lett.
359
147-153
2014
Methanosarcina mazei (P0CW05), Methanosarcina mazei, Methanosarcina mazei DSM 3647 (P0CW05)
Manually annotated by BRENDA team