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Literature summary for 2.7.2.1 extracted from
- Cloning of a gene encoding acetate kinase from Methanosarcina mazei 2-P isolated from a Japanese paddy field soil (2002), Curr. Microbiol., 45, 390-393.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functionally expressed of the His6-tagged enzyme in Escherichia coli JM109 | Methanosarcina mazei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44500 | - |
x * 44500, calculated from sequence | Methanosarcina mazei |
| 45000 | - |
x * 45000, SDS-PAGE | Methanosarcina mazei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Methanosarcina mazei | acetate kinase is a key enzymes of acetate metabolism of anaerobes | ADP + acetyl phosphate | - |
? |  |
| ATP + acetate | Methanosarcina mazei 2-P | acetate kinase is a key enzymes of acetate metabolism of anaerobes | ADP + acetyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina mazei | P0CW04 | - |
- |
| Methanosarcina mazei 2-P | P0CW04 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina mazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Methanosarcina mazei | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | acetate kinase is a key enzymes of acetate metabolism of anaerobes | Methanosarcina mazei | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | - |
Methanosarcina mazei 2-P | ADP + acetyl phosphate | - |
? | |
| ATP + acetate | acetate kinase is a key enzymes of acetate metabolism of anaerobes | Methanosarcina mazei 2-P | ADP + acetyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Methanosarcina mazei |
| ? | x * 44500, calculated from sequence | Methanosarcina mazei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACK | - |
Methanosarcina mazei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Methanosarcina mazei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 43 | 20°C: 75% of maximal activity, 43°C: 45% of maximal activity | Methanosarcina mazei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methanosarcina mazei | calculated from sequence | - |
5.7 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | acetate kinase is a key enzyme of acetate metabolism of anaerobes | Methanosarcina mazei |
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