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Information on EC 2.7.1.82 - ethanolamine kinase and Organism(s) Arabidopsis thaliana and UniProt Accession O81024

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Arabidopsis thaliana
UNIPROT: O81024 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ethanolamine kinase, etnk1, etnk2, etn kinase, ethanolamine kinase 1, ethanolamine kinase 2, treki1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
choline/ethanolamine kinase
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EKI
-
-
-
-
EKI1
-
-
-
-
ethanolamine phosphokinase
-
-
-
-
EtnK
-
-
-
-
kinase, ethanolamine (phosphorylating)
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:ethanolamine O-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9075-78-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
-
-
-
?
additional information
?
-
enzyme CEK4 is a bifunctional choline/ethanolamine kinase
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
-
-
-
?
additional information
?
-
enzyme CEK4 is a bifunctional choline/ethanolamine kinase
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression
Manually annotated by BRENDA team
high expression level of isozyme CEK4 in male reproductive organs
Manually annotated by BRENDA team
additional information
enzyme CEK4 is preferentially expressed in the vasculature, organ boundaries, and mature embryos. Isozyme CEK4 shows increased expression in senescent tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
the enzyme has no transmembrane domain
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzmye belongs to the choline/ethanolamine kinase (CEK) family catalyzing the initial steps of phospholipid biosynthesis
malfunction
knocking out of CEK4 caused an embryo-lethal phenotype with defective embryo development, which is complemented by transformation of genomic CEK4. Overexpression of enzyme CEK4 in wild-type Arabidopsis thaliana increases the levels of phosphatidylcholine in seedlings and mature siliques and of major membrane lipids in seedlings and triacylglycerol in mature siliques
metabolism
the enzyme catalyzes the initial steps of phospholipid biosynthesis
physiological function
enzyme CEK4 may be the plasma membrane-localized isoform of the CEK family involved in the rate-limiting step of phosphatidylcholine biosynthesis and appears to be required for embryo development in Arabidopsis thaliana
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EKI_ARATH
374
0
42860
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
x * 40000, about, sequence calculation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, about, sequence calculation
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
construction of CEK4 knockout mutant, the CEK4 knockout causes embryonic lethality, and developmental arrest after the heart stage, with no defect in the pollen
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 (DE3) cells
gene At2g26830, PCR-based genotyping of CEK family members CEK1-4
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lin, Y.C.; Liu, Y.C.; Nakamura, Y.
The choline/ethanolamine kinase family in Arabidopsis: essential role of CEK4 in phospholipid biosynthesis and embryo development
Plant Cell
27
1497-1511
2015
Arabidopsis thaliana (O81024)
Manually annotated by BRENDA team
Lin, Y.C.; Araguirang, G.E.; Ngo, A.H.; Lin, K.T.; Angkawijaya, A.E.; Nakamura, Y.
The four Arabidopsis choline/ethanolamine kinase isozymes play distinct roles in metabolism and development
Plant Physiol.
183
152-166
2020
Arabidopsis thaliana (O81024)
Manually annotated by BRENDA team