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Literature summary for 2.7.1.82 extracted from

  • Lin, Y.C.; Liu, Y.C.; Nakamura, Y.
    The choline/ethanolamine kinase family in Arabidopsis: essential role of CEK4 in phospholipid biosynthesis and embryo development (2015), Plant Cell, 27, 1497-1511.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene At2g26830, PCR-based genotyping of CEK family members CEK1-4 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of CEK4 knockout mutant, the CEK4 knockout causes embryonic lethality, and developmental arrest after the heart stage, with no defect in the pollen Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme has no transmembrane domain Arabidopsis thaliana
-
-
plasma membrane mainly Arabidopsis thaliana 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, about, sequence calculation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ethanolamine Arabidopsis thaliana
-
ADP + O-phosphoethanolamine
-
?
additional information Arabidopsis thaliana enzyme CEK4 is a bifunctional choline/ethanolamine kinase ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O81024 gene At2g26830
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Arabidopsis thaliana
-
additional information enzyme CEK4 is preferentially expressed in the vasculature, organ boundaries, and mature embryos. Isozyme CEK4 shows increased expression in senescent tissues Arabidopsis thaliana
-
pollen high expression level of isozyme CEK4 in male reproductive organs Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
vascular system
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ethanolamine
-
Arabidopsis thaliana ADP + O-phosphoethanolamine
-
?
additional information enzyme CEK4 is a bifunctional choline/ethanolamine kinase Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, about, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CEK4
-
Arabidopsis thaliana
choline/ethanolamine kinase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana

General Information

General Information Comment Organism
evolution the enzmye belongs to the choline/ethanolamine kinase (CEK) family catalyzing the initial steps of phospholipid biosynthesis Arabidopsis thaliana
malfunction knocking out of CEK4 caused an embryo-lethal phenotype with defective embryo development, which is complemented by transformation of genomic CEK4. Overexpression of enzyme CEK4 in wild-type Arabidopsis thaliana increases the levels of phosphatidylcholine in seedlings and mature siliques and of major membrane lipids in seedlings and triacylglycerol in mature siliques Arabidopsis thaliana
metabolism the enzyme catalyzes the initial steps of phospholipid biosynthesis Arabidopsis thaliana
physiological function enzyme CEK4 may be the plasma membrane-localized isoform of the CEK family involved in the rate-limiting step of phosphatidylcholine biosynthesis and appears to be required for embryo development in Arabidopsis thaliana Arabidopsis thaliana