Cloned (Comment) | Organism |
---|---|
gene At2g26830, PCR-based genotyping of CEK family members CEK1-4 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of CEK4 knockout mutant, the CEK4 knockout causes embryonic lethality, and developmental arrest after the heart stage, with no defect in the pollen | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme has no transmembrane domain | Arabidopsis thaliana | - |
- |
plasma membrane | mainly | Arabidopsis thaliana | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, about, sequence calculation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | Arabidopsis thaliana | - |
ADP + O-phosphoethanolamine | - |
? | |
additional information | Arabidopsis thaliana | enzyme CEK4 is a bifunctional choline/ethanolamine kinase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O81024 | gene At2g26830 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Arabidopsis thaliana | - |
additional information | enzyme CEK4 is preferentially expressed in the vasculature, organ boundaries, and mature embryos. Isozyme CEK4 shows increased expression in senescent tissues | Arabidopsis thaliana | - |
pollen | high expression level of isozyme CEK4 in male reproductive organs | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
vascular system | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | - |
Arabidopsis thaliana | ADP + O-phosphoethanolamine | - |
? | |
additional information | enzyme CEK4 is a bifunctional choline/ethanolamine kinase | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, about, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
CEK4 | - |
Arabidopsis thaliana |
choline/ethanolamine kinase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the enzmye belongs to the choline/ethanolamine kinase (CEK) family catalyzing the initial steps of phospholipid biosynthesis | Arabidopsis thaliana |
malfunction | knocking out of CEK4 caused an embryo-lethal phenotype with defective embryo development, which is complemented by transformation of genomic CEK4. Overexpression of enzyme CEK4 in wild-type Arabidopsis thaliana increases the levels of phosphatidylcholine in seedlings and mature siliques and of major membrane lipids in seedlings and triacylglycerol in mature siliques | Arabidopsis thaliana |
metabolism | the enzyme catalyzes the initial steps of phospholipid biosynthesis | Arabidopsis thaliana |
physiological function | enzyme CEK4 may be the plasma membrane-localized isoform of the CEK family involved in the rate-limiting step of phosphatidylcholine biosynthesis and appears to be required for embryo development in Arabidopsis thaliana | Arabidopsis thaliana |