Information on EC 2.7.1.72 - streptomycin 6-kinase
for references in articles please use BRENDA:EC2.7.1.72Word Map on EC 2.7.1.72

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The enzyme appears in viruses and cellular organisms
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ATP + streptomycin = ADP + streptomycin 6-phosphate
dATP can replace ATP, dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors
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phospho group transfer
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streptomycin biosynthesis
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Streptomycin biosynthesis
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Biosynthesis of antibiotics
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ATP:streptomycin 6-phosphotransferase
dATP can replace ATP; and dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors.
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aminoglycoside phosphotransferase
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kinase, streptidine (phosphorylating)
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kinase, streptomycin 6- (phosphorylating)
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streptidine kinase
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streptidine kinase (phosphorylating)
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streptomycin 6-kinase (phosphorylating)
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streptomycin 6-O-phosphotransferase
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streptomycin 6-phosphotransferase
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streptomycin phosphotransferase
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strain ATCC 14607
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strain ATCC 11062
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physiological function
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the enzyme confers resistance to streptomycin
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3'-deoxydihydrostreptomycin + ATP
ADP + 3'-deoxydihydrostreptomycin 6-phosphate
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ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
ATP + streptomycin
ADP + streptomycin 6-phosphate
dATP + streptomycin
dADP + streptomycin 6-phosphate
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dihydrostreptomycin 3''-alpha-phosphate + ATP
ADP + dihydrostreptomycin 3''-alpha-phosphate 6-phosphate
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dihydrostreptomycin 3''-phosphate + ATP
ADP + dihydrostreptomycin 3'',6-bisphosphate
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streptidine + ATP
streptidine 6-phosphate + ADP
additional information
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ATP + dihydrostreptomycin

ADP + dihydrostreptomycin 6-phosphate
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ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
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ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
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ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
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ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
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ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
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?
ATP + streptomycin

ADP + streptomycin 6-phosphate
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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streptomycin is secreted
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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streptomycin is secreted
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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detoxification of a potent inhibitor of protein biosynthesis
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis
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?
streptidine + ATP

streptidine 6-phosphate + ADP
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streptidine + ATP
streptidine 6-phosphate + ADP
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O-phosphorylstreptidine + ADP
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streptidine + ATP
streptidine 6-phosphate + ADP
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streptidine + ATP
streptidine 6-phosphate + ADP
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additional information

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no activity with GTP, CTP, UTP
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additional information
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no activity with streptomycin derivatives that contain 3'-alpha-aldehyde groups
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additional information
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no activity with N-amidinostreptamine, N'-amidinostreptamine, N-amidinoinosamine, dTTP
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additional information
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no activity with GTP, CTP, UTP
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additional information
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no activity with GTP, CTP, UTP
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ATP + streptomycin
ADP + streptomycin 6-phosphate
ATP + streptomycin

ADP + streptomycin 6-phosphate
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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streptomycin is secreted
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ATP + streptomycin
ADP + streptomycin 6-phosphate
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streptomycin is secreted
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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detoxification of a potent inhibitor of protein biosynthesis
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis
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?
ATP + streptomycin
ADP + streptomycin 6-phosphate
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self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis
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?
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ATP

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Co2+
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10 mM, rather less stimulatory than Mg2+
Cu2+
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10 mM, rather less stimulatory than Mg2+
Fe2+
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10 mM, slight stimulation
Zn2+
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10 mM, slight stimulation
additional information
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not affected by Ca2+, 0.2-100 mM
Mg2+

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required
Mg2+
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maximal activity with 10 mM; required
Mn2+

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can replace Mg2+ in activation
Mn2+
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can replace Mg2+ in activation
Mn2+
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10 mM, slight stimulation
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AgNO3
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strong inhibition
diphosphate
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noncompetitive
additional information
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no inhibition by sulfhydryl reagents, formamidine disulfide, p-chloromercuribenzoate
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dihydrostreptomycin
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0.4
ATP

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1.03
ATP
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at pH 7.5 and 25°C
0.38
streptomycin

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at pH 7.5 and 25°C
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1.7
ATP
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at pH 7.5 and 25°C
1.7
streptomycin
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at pH 7.5 and 25°C
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4.5
streptomycin
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at pH 7.5 and 25°C
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additional information
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assay methods
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additional information
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pI: 6.6
7.4

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assay at
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brenda
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brenda
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30000
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1 * 30000, SDS-PAGE
36000
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1 * 36000, SDS-PAGE
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monomer

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1 * 30000, SDS-PAGE
monomer
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1 * 36000, SDS-PAGE
monomer
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1 * 36000, SDS-PAGE
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Ni-NTA column chromatography
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expressed in Escherichia coli Rosetta(DE3)pLysS cells
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STRA_STRGA
307
33154
Swiss-Prot
STRA_STRGR
307
33244
Swiss-Prot
D9TCR4_MICAI
Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / BCRC 12538 / CBS 129.76 / JCM 10878 / NBRC 16125 / NRRL B-16091 / INA 9442)
290
31403
TrEMBL
I0LB82_9ACTN
288
31945
TrEMBL
A0A1L7F8X8_9PSEU
316
33995
TrEMBL
A0A2H1L322_9MICO
339
37688
TrEMBL
E8W6J5_STRFA
Streptomyces pratensis (strain ATCC 33331 / IAF-45CD)
303
32718
TrEMBL
A0A2H1K763_9MICO
339
37172
TrEMBL
A0A0D6IBD5_MYCSM
302
33452
TrEMBL
G0Q7L8_9ACTN
307
33228
TrEMBL
A0A2H1JEM6_9MICO
339
36714
TrEMBL
D3Q2G9_STANL
Stackebrandtia nassauensis (strain DSM 44728 / NRRL B-16338 / NBRC 102104 / LLR-40K-21)
309
33653
TrEMBL
A0QXZ6_MYCS2
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
309
34349
TrEMBL
A0A1B2GLC3_STRNR
308
33332
TrEMBL
A1R510_PAEAT
Paenarthrobacter aurescens (strain TC1)
336
37080
TrEMBL
A0A1Q8LYG6_9PSEU
297
31390
TrEMBL
A0A1Y5P1D7_9MYCO
294
33198
TrEMBL
A0A2C9T286_9MYCO
294
32737
TrEMBL
A0A1C6YXH2_HAFAL
267
29931
TrEMBL
A0A0B8NDT6_9NOCA
78
8166
TrEMBL
A0A2H1IAM1_9MICO
339
36785
TrEMBL
M9U8W1_9ACTN
93
10662
TrEMBL
F2RG02_STRVP
Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
298
32102
TrEMBL
A0A1Q8JQ71_9PSEU
294
31357
TrEMBL
A0A1D7W5C4_BRELN
339
36810
TrEMBL
A0A2H1IRL9_9MICO
339
36794
TrEMBL
G4HW95_MYCRH
297
33315
TrEMBL
A0A1J1ECH9_LYSEN
271
30427
TrEMBL
Q0SDP8_RHOJR
Rhodococcus jostii (strain RHA1)
75
8563
TrEMBL
A0A1D8BZB3_9PSEU
291
31486
TrEMBL
A0A1L7F9X8_9PSEU
298
31551
TrEMBL
A0A2P9I4J2_9ACTN
318
33940
TrEMBL
A0A0A1G307_9MYCO
318
35734
TrEMBL
A0A2H1IZ65_9MICO
339
36748
TrEMBL
A0A0N9Y739_MYCFO
318
35748
TrEMBL
G0PWL8_9ACTN
302
31960
TrEMBL
B5GMY7_STRC2
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
312
33629
TrEMBL
N0D167_9ACTN
321
33670
TrEMBL
L1KNV1_9ACTN
292
32658
TrEMBL
A0A1X6XKG7_9MICO
339
37580
TrEMBL
E6SE91_INTC7
Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)
312
34106
TrEMBL
A0A1B2GPW6_STRNR
302
32165
TrEMBL
D7AUR1_NOCDD
Nocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / CIP 107115 / JCM 7437 / KCTC 9190 / NBRC 14626 / NCTC 10488 / NRRL B-5397 / IMRU 509)
307
34053
TrEMBL
A0A0M8KRR5_9MICO
307
33433
TrEMBL
A0A077LVB2_9MICO
302
33059
TrEMBL
A0A2H1J5J6_BRELN
339
37129
TrEMBL
Q0SDP9_RHOJR
Rhodococcus jostii (strain RHA1)
186
20536
TrEMBL
A0A1Q8LB99_9PSEU
297
31360
TrEMBL
A0A169NQ41_STRLU
303
32329
TrEMBL
A0A2H1KGG4_9MICO
339
37177
TrEMBL
Q53ID0_STRGR
343
37291
TrEMBL
A0A2H1I4K5_9MICO
339
37624
TrEMBL
D2PUZ7_KRIFD
Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399)
304
34298
TrEMBL
L7F4Y2_9ACTN
295
32869
TrEMBL
N0CJS9_9ACTN
382
39768
TrEMBL
W7JDF2_9PSEU
273
28415
TrEMBL
I7G2Y8_MYCS2
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
309
34349
TrEMBL
A0A0K3BFQ9_9PSEU
302
33695
TrEMBL
Q7X3B0_9BACT
278
30824
TrEMBL
A0A0B8NFG6_9NOCA
220
24038
TrEMBL
A0A1D8BXX4_9PSEU
315
33980
TrEMBL
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Walker, J.B.; Walker, M.S.
Streptomycin biosynthesis. Enzymatic synthesis of O-phosphorylstreptidine from streptidine and adenosinetriphosphate
Biochim. Biophys. Acta
148
335-341
1967
Streptomyces bikiniensis
brenda
Walker, J.B.; Skorvaga, M.
Phosphorylation of streptomycin and dihydrostreptomycin by Streptomyces. Enzymatic synthesis of different diphosphorylated derivatives
J. Biol. Chem.
248
2435-2440
1973
Streptomyces bikiniensis
brenda
Walker, J.B.
ATP:streptomycin 6-phosphotransferase
Methods Enzymol.
43
628-632
1975
Streptomyces bikiniensis, Streptomyces glebosus
brenda
Sugiyama, M.; Sakamoto, M.; Mochizuki, H.; Nimi, O.; Nomi, R.
Purification and characterization of streptomycin 6-kinase, an enzyme implicated in self-protection of a streptomycin-producing micro-organism
J. Gen. Microbiol.
129
1683-1687
1983
Streptomyces griseus, Streptomyces griseus HUT 6037
brenda
Ashenafi, M.; Ammosova, T.; Nekhai, S.; Byrnes, W.M.
Purification and characterization of aminoglycoside phosphotransferase APH(6)-Id, a streptomycin-inactivating enzyme
Mol. Cell. Biochem.
387
207-216
2014
Escherichia coli
brenda
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