Information on EC 2.7.1.72 - streptomycin 6-kinase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.7.1.72
-
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RECOMMENDED NAME
GeneOntology No.
streptomycin 6-kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + streptomycin = ADP + streptomycin 6-phosphate
show the reaction diagram
dATP can replace ATP, dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
streptomycin biosynthesis
-
-
Streptomycin biosynthesis
-
-
Biosynthesis of antibiotics
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:streptomycin 6-phosphotransferase
dATP can replace ATP; and dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors.
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CAS REGISTRY NUMBER
COMMENTARY hide
37278-11-8
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain ATCC 14607
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-
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme confers resistance to streptomycin
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-deoxydihydrostreptomycin + ATP
ADP + 3'-deoxydihydrostreptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
show the reaction diagram
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
dATP + streptomycin
dADP + streptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-alpha-phosphate + ATP
ADP + dihydrostreptomycin 3''-alpha-phosphate 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-phosphate + ATP
ADP + dihydrostreptomycin 3'',6-bisphosphate
show the reaction diagram
-
-
-
-
?
streptidine + ATP
streptidine 6-phosphate + ADP
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
10 mM, rather less stimulatory than Mg2+
Cu2+
-
10 mM, rather less stimulatory than Mg2+
Fe2+
-
10 mM, slight stimulation
Zn2+
-
10 mM, slight stimulation
additional information
-
not affected by Ca2+, 0.2-100 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
-
competitive
ADP
-
noncompetitive
AgNO3
-
strong inhibition
AMP
-
noncompetitive
dihydrostreptomycin
-
-
diphosphate
-
noncompetitive
EDTA
-
strong inhibition
streptomycin
-
-
additional information
-
no inhibition by sulfhydryl reagents, formamidine disulfide, p-chloromercuribenzoate
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 1.03
ATP
11
dihydrostreptomycin
-
-
28
streptidine
-
-
0.38 - 3.5
streptomycin
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
ATP
-
at pH 7.5 and 25°C
1.7
streptomycin
-
at pH 7.5 and 25°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5
streptomycin
-
at pH 7.5 and 25°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
adenosine
-
-
3
diphosphate
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.35
-
purified enzyme
additional information
-
assay methods
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
assay at, method 1
additional information
-
pI: 6.6
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
1 * 30000, SDS-PAGE
31400
-
gel filtration
36000
-
1 * 36000, SDS-PAGE
38000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta(DE3)pLysS cells
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.72)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)