Information on EC 2.7.1.72 - streptomycin 6-kinase

for references in articles please use BRENDA:EC2.7.1.72
Word Map on EC 2.7.1.72
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
2.7.1.72
-
top print hide
RECOMMENDED NAME
GeneOntology No.
streptomycin 6-kinase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + streptomycin = ADP + streptomycin 6-phosphate
show the reaction diagram
dATP can replace ATP, dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
streptomycin biosynthesis
-
-
Streptomycin biosynthesis
-
-
Biosynthesis of antibiotics
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
ATP:streptomycin 6-phosphotransferase
dATP can replace ATP; and dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
37278-11-8
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain ATCC 14607
-
-
Manually annotated by BRENDA team
top print hide
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme confers resistance to streptomycin
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-deoxydihydrostreptomycin + ATP
ADP + 3'-deoxydihydrostreptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
show the reaction diagram
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
dATP + streptomycin
dADP + streptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-alpha-phosphate + ATP
ADP + dihydrostreptomycin 3''-alpha-phosphate 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-phosphate + ATP
ADP + dihydrostreptomycin 3'',6-bisphosphate
show the reaction diagram
-
-
-
-
?
streptidine + ATP
streptidine 6-phosphate + ADP
show the reaction diagram
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
10 mM, rather less stimulatory than Mg2+
Cu2+
-
10 mM, rather less stimulatory than Mg2+
Fe2+
-
10 mM, slight stimulation
Zn2+
-
10 mM, slight stimulation
additional information
-
not affected by Ca2+, 0.2-100 mM
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
-
competitive
ADP
-
noncompetitive
AgNO3
-
strong inhibition
AMP
-
noncompetitive
dihydrostreptomycin
-
-
diphosphate
-
noncompetitive
EDTA
-
strong inhibition
streptomycin
-
-
additional information
-
no inhibition by sulfhydryl reagents, formamidine disulfide, p-chloromercuribenzoate
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 1.03
ATP
11
dihydrostreptomycin
-
-
28
streptidine
-
-
0.38 - 3.5
streptomycin
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
ATP
-
at pH 7.5 and 25°C
1.7
streptomycin
-
at pH 7.5 and 25°C
top print hide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5
streptomycin
-
at pH 7.5 and 25°C
top print hide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
adenosine
-
-
3
diphosphate
-
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.35
-
purified enzyme
additional information
-
assay methods
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
assay at, method 1
additional information
-
pI: 6.6
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
1 * 30000, SDS-PAGE
31400
-
gel filtration
36000
-
1 * 36000, SDS-PAGE
38000
-
gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
-
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta(DE3)pLysS cells
-
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.72)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)