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Literature summary for 2.7.1.72 extracted from
- Purification and characterization of streptomycin 6-kinase, an enzyme implicated in self-protection of a streptomycin-producing micro-organism (1983), J. Gen. Microbiol., 129, 1683-1687.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | competitive | Streptomyces griseus |  |
| ADP | noncompetitive | Streptomyces griseus | |
| AgNO3 | strong inhibition | Streptomyces griseus | |
| AMP | noncompetitive | Streptomyces griseus | |
| ATP | - |
Streptomyces griseus | |
| diphosphate | noncompetitive | Streptomyces griseus | |
| EDTA | strong inhibition | Streptomyces griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
ATP | - |
Streptomyces griseus | |
| 3.5 | - |
streptomycin | - |
Streptomyces griseus | |
| 11 | - |
dihydrostreptomycin | - |
Streptomyces griseus | |
| 28 | - |
streptidine | - |
Streptomyces griseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, rather less stimulatory than Mg2+ | Streptomyces griseus | |
| Cu2+ | 10 mM, rather less stimulatory than Mg2+ | Streptomyces griseus | |
| Fe2+ | 10 mM, slight stimulation | Streptomyces griseus | |
| Mg2+ | required | Streptomyces griseus | |
| Mg2+ | maximal activity with 10 mM | Streptomyces griseus | |
| Mn2+ | 10 mM, slight stimulation | Streptomyces griseus | |
| additional information | not affected by Ca2+, 0.2-100 mM | Streptomyces griseus | |
| Zn2+ | 10 mM, slight stimulation | Streptomyces griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
1 * 36000, SDS-PAGE | Streptomyces griseus |
| 38000 | - |
gel filtration | Streptomyces griseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + streptomycin | Streptomyces griseus | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | ADP + streptomycin 6-phosphate | - |
? | |
| ATP + streptomycin | Streptomyces griseus HUT 6037 | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | ADP + streptomycin 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
| Streptomyces griseus HUT 6037 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces griseus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.35 | - |
purified enzyme | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + dihydrostreptomycin | - |
Streptomyces griseus | ADP + dihydrostreptomycin 6-phosphate | - |
? | |
| ATP + dihydrostreptomycin | - |
Streptomyces griseus HUT 6037 | ADP + dihydrostreptomycin 6-phosphate | - |
? | |
| ATP + streptomycin | - |
Streptomyces griseus | ADP + streptomycin 6-phosphate | - |
? | |
| ATP + streptomycin | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | Streptomyces griseus | ADP + streptomycin 6-phosphate | - |
? | |
| ATP + streptomycin | - |
Streptomyces griseus HUT 6037 | ADP + streptomycin 6-phosphate | - |
? | |
| ATP + streptomycin | self-protection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis | Streptomyces griseus HUT 6037 | ADP + streptomycin 6-phosphate | - |
? | |
| additional information | no activity with GTP, CTP, UTP | Streptomyces griseus | ? | - |
? | |
| additional information | no activity with GTP, CTP, UTP | Streptomyces griseus HUT 6037 | ? | - |
? | |
| streptidine + ATP | - |
Streptomyces griseus | streptidine 6-phosphate + ADP | - |
? | |
| streptidine + ATP | - |
Streptomyces griseus HUT 6037 | streptidine 6-phosphate + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.6 | Streptomyces griseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptomyces griseus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
adenosine | - |
Streptomyces griseus | |
| 3 | - |
diphosphate | - |
Streptomyces griseus | |
| 5 | - |
ADP | - |
Streptomyces griseus | |
| 6 | - |
ATP | - |
Streptomyces griseus | |
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