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Information on EC 2.7.1.166 - 3-deoxy-D-manno-octulosonic acid kinase and Organism(s) Pasteurella multocida and UniProt Accession Q9CLD5

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IUBMB Comments
The enzyme phosphorylates the 4-OH position of KDO in (KDO)-lipid IVA.
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Pasteurella multocida
UNIPROT: Q9CLD5
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The taxonomic range for the selected organisms is: Pasteurella multocida
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
kdo kinase, 3-deoxy-d-manno-octulosonic acid kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Kdo kinase
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:(KDO)-lipid IVA 3-deoxy-alpha-D-manno-oct-2-ulopyranose 4-phosphotransferase
The enzyme phosphorylates the 4-OH position of KDO in (KDO)-lipid IVA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + ATP
3-deoxy-4-O-phosphono-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + ADP
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + ATP
3-deoxy-4-O-phosphono-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + ADP
show the reaction diagram
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Pasteurella multocida contains two simultaneously expressed inner core structures: most glycoforms contain a single phosphorylated 3-deoxy-D-manno-octulosonic acid molecule that is frequently substituted with a phosphoethanolamine residue attached to the phosphate group (glycoform A), the alternative inner core lipopolysaccharide structure contains two unphosphorylated KDO residues (glycoform B). (KDO)-lipid IV(A) = 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose. (4-O-phospho-KDO)-lipid IV(A) = 3-deoxy-4-O-phosphono-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose
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-
?
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
Kdo kinase mutant produces only glycoform B (containing two unphosphorylated Kdo residues) and not glycoform A (containing a single phosphorylated 3-deoxy-D-manno-octulosonic acid molecule that is frequently substituted with a phosphoethanolamine residue attached to the phosphate group)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A112V
spontaneous KdkA mutant strain isolated from birds inoculated with high doses of a heptosyltransferase hptA mutant. The birds developed fowl cholera and the Pasteurella multocida isolates recovered from diseased birds no longer express truncated lipopolysaccharide. Mutation results in low level of enzymic acitivity
D193N
spontaneous KdkA mutant strain isolated from birds inoculated with high doses of a heptosyltransferase hptA mutant. The birds developed fowl cholera and the Pasteurella multocida isolates recovered from diseased birds no longer express truncated lipopolysaccharide. Mutation leads to complete loss of enzymic acitivity
H168Y
spontaneous KdkA mutant strain isolated from birds inoculated with high doses of a heptosyltransferase hptA mutant. The birds developed fowl cholera and the Pasteurella multocida isolates recovered from diseased birds no longer express truncated lipopolysaccharide. Residue is critical for Kdo kinase function and therefore glycoform A lipopolysaccharide assembly
R123P
spontaneous KdkA mutant strain isolated from birds inoculated with high doses of a heptosyltransferase hptA mutant. The birds developed fowl cholera and the Pasteurella multocida isolates recovered from diseased birds no longer express truncated lipopolysaccharide. Mutation is likely to have a significant impact on the secondary structure and probably affects the hinge action of the linker region
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Harper, M.; Boyce, J.D.; Cox, A.D.; St. Michael, F.; Wilkie, I.W.; Blackall, P.J.; Adler, B.
Pasteurella multocida expresses two lipopolysaccharide glycoforms simultaneously, but only a single form is required for virulence: identification of two acceptor-specific heptosyl I transferases
Infect. Immun.
75
3885-3893
2007
Pasteurella multocida, Pasteurella multocida VP161
Manually annotated by BRENDA team
Harper, M.; Cox, A.D.; St Michael, F.; Ford, M.; Wilkie, I.W.; Adler, B.; Boyce, J.D.
Natural selection in the chicken host identifies 3-deoxy-D-manno-octulosonic acid kinase residues essential for phosphorylation of Pasteurella multocida lipopolysaccharide
Infect. Immun.
78
3669-3677
2010
Pasteurella multocida (D9MNH9), Pasteurella multocida
Manually annotated by BRENDA team