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Information on EC 2.7.1.144 - tagatose-6-phosphate kinase
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2.7.1.144 tagatose-6-phosphate kinaseSpecify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
D-tagatose 6-phosphate kinase, D-tagatose-6-phosphate kinase, Lacc, phosphotagatokinase, tagatose 6-phosphate kinase (phosphorylating), 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-tagatose 6-phosphate kinase
Lacc
tagatose 6-phosphate kinase (phosphorylating)
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D-tagatose 6-phosphate kinase
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Lacc
belongs to the pfkB family of carbohydrate kinases, which form a subset of the ribokinase superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
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ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
mechanism suggested
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-tagatose-6-phosphate 1-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY
39434-00-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose 6-phosphate
ADP + D-fructose 1,6-bisphosphate
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-
-
?
CTP + D-tagatose 6-phosphate
CDP + D-tagatose 1,6-bisphosphate
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-
-
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?
GTP + D-tagatose 6-phosphate
GDP + D-tagatose 1,6-bisphosphate
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?
ITP + D-tagatose 6-phosphate
IDP + D-tagatose 1,6-bisphosphate
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-
-
-
?
TTP + D-tagatose 6-phosphate
TDP + D-tagatose 1,6-bisphosphate
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-
-
-
?
UTP + D-tagatose 6-phosphate
UDP + D-tagatose 1,6-bisphosphate
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-
-
-
?
additional information
?
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enzyme efficient biocatalytic synthesis system is developed for the phosphorylation of D-tagatose 6-phosphate at its C1-position using the recombinant D-tagatose 6-phosphate kinase and the phosphoenolpyruvate/pyruvate kinase-system for ATP regeneration. This straightforward and scalable one-step biocatalytic synthesis of D-tagatose 1,6-diphosphate is successfully scaled up to the gram scale. Method evaluation and optimization, production analysis by NMR spectroscopy, overview
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ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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inducible by D-galactose
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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involved in the catabolism of lactose and D-galactose
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
second step of the D-tagatose-6-phosphate pathway which is the direct catabolic pathway for lactose
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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induced by growth on lactose
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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-
-
?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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inducible by D-galactose
-
-
?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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involved in the catabolism of lactose and D-galactose
-
-
?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
second step of the D-tagatose-6-phosphate pathway which is the direct catabolic pathway for lactose
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-
?
ATP + D-tagatose 6-phosphate
ADP + D-tagatose 1,6-bisphosphate
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induced by growth on lactose
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mg2+
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absolute requirement for a divalent cation, a Mg2+ : ATP ratio of 2.0-2.5 is required for maximal activity
Mg2+
required, two ions bound to the active site when substrate and cofactor are bound
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 9.5
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pH 7.5: about 75% of maximal activity, pH 9.5: about 55% of maximal activity, glycylglycine buffer
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
D-tagatose 1,6-diphosphate is an important metabolite which can be formed via the classical tagatose 6-phosphate pathway or via the tagatose 1-phosphate pathway
physiological function
enzyme LacC catalyses the efficient biocatalytic synthesis of D-tagatose 1,6-diphosphate via phosphorylation of D-tagatose 6-phosphate
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
dimer
gel filtration data, crystal structure analysis, two monomers associate via interactions between their lid domains, which come together to form a beta-barrel structure known as a beta-clasp
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
high resolution structures of D-tagatose-6-phosphate kinase (LacC) in two crystal forms. The structures define LacC in apoform, in binary complexes with ADP or the cofactor analogue adenosine 5'-(beta,gamma-imino)triphosphate, and in a ternary complex with adenosine 5'-(beta,gamma-imino)triphosphate and D-tagatose-6-phosphate. LacC adopts a closed structure required for phosphoryl transfer only when both substrate and co-factor are bound
native and L124M, L125M double mutant crystallized by hanging drop vapor diffusion method in apoform, in binary complexes with ADP or the cofactor analogue AMP-PNP, and in a ternary complex with AMP-PNP and D-tagatose-6-phosphate, wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
L124M/L125M
wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 20% v/v glycerol, 0.2% v/v 2-mercaptoethanol, stable for several months
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli strain BL21(DE3)
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene lacC, codon-optimized, recombinant expression in Escherichia coli strain BL21(DE3)
native enzyme and L124M, L125M double mutant expressed in Escherichia coli BL21(DE3)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Van Rooijen, R.J.; van Schalkwijk, S.; de Vos, W.M.
Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactose operon of Lactococcus lactis
J. Biol. Chem.
266
7176-7181
1991
Lactobacillus delbrueckii subsp. lactis
brenda
Anderson, R.L.; Bissett, D.L.
D-Tagatose-6-phosphate kinase from Staphylococcus aureus
Methods Enzymol.
90
87-91
1982
Staphylococcus aureus
-
brenda
Bissett, D.L.; Anderson, R.L.
Lactose and D-galactose metabolism in Staphylococcus aureus. III: Purification and properties of D-tagatose-6-phosphate kinase
J. Biol. Chem.
255
8745-8749
1980
Staphylococcus aureus
brenda
Hamilton, I.R.; Lebtag, H.
Lactose metabolism by Streptococcus mutans: evidence for induction of the tagatose 6-phosphate pathway
J. Bacteriol.
140
1102-1104
1979
Streptococcus mutans
brenda
Schleifer, K.H.; Hartinger, A.; Götz, F.
Occurence of D-tagatose-6-phosphate pathway of D-galactose metabolism among Staphylococci
FEMS Microbiol. Lett.
3
9-11
1978
Staphylococcus aureus, no activity in Lactobacillus plantarum, no activity in Streptococcus faecalis, Staphylococcus epidermidis, Staphylococcus hominis
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brenda
Jagusztyn-Krynicka, E.K.; Hansen, J.B.; Crow, V.L.; Thomas, T.D.; Honeyman, A.L.; Curtiss, R.III.
Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster
J. Bacteriol.
174
6152-6158
1992
Streptococcus mutans
brenda
Miallau, L.; Hunter, W.N.; McSweeney, S.M.; Leonard, G.A.
Structures of Staphylococcus aureus D-tagatose-6-phosphate kinase implicate domain motions in specificity and mechanism
J. Biol. Chem.
282
19948-19957
2007
Staphylococcus aureus (P0A0B9), Staphylococcus aureus
brenda
Schoenenberger, B.; Kind, S.; Meier, R.; Eggert, T.; Obkircher, M.; Wohlgemuth, R.
Efficient biocatalytic synthesis of D-tagatose 1,6-diphosphate by LacC-catalysed phosphorylation of D-tagatose 6-phosphate
Biocatal. Biotransform.
38
53-63
2020
Lactococcus lactis subsp. lactis (P23391)
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brenda
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