BRENDA - Enzyme Database
show all sequences of 2.7.1.144

Lactose and D-galactose metabolism in Staphylococcus aureus. III: Purification and properties of D-tagatose-6-phosphate kinase

Bissett, D.L.; Anderson, R.L.; J. Biol. Chem. 255, 8745-8749 (1980)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NH4+
33.3 mM activates
Staphylococcus aureus
General Stability
General Stability
Organism
2-mercaptoethanol stabilizes during purification
Staphylococcus aureus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Li+
33.3 mM, 65% inhibition
Staphylococcus aureus
Na+
33.3 mM, 31% inhibition
Staphylococcus aureus
NEM
-
Staphylococcus aureus
phosphate
at high concentrations
Staphylococcus aureus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
D-tagatose 6-phosphate
-
Staphylococcus aureus
0.15
-
D-fructose 6-phosphate
-
Staphylococcus aureus
0.16
-
ATP
-
Staphylococcus aureus
0.17
-
ITP
-
Staphylococcus aureus
0.4
-
GTP
-
Staphylococcus aureus
0.9
-
UTP
-
Staphylococcus aureus
18
-
CTP
-
Staphylococcus aureus
20
-
TTP
-
Staphylococcus aureus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
8% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
Co2+
54% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
K+
33.3 mM activates
Staphylococcus aureus
Mg2+
absolute requirement for a divalent cation, a Mg2+ : ATP ratio of 2.0-2.5 is required for maximal activity
Staphylococcus aureus
Mn2+
27% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
Ni2+
15% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
Rb+
33.3 mM activates
Staphylococcus aureus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
alpha2, 2 * 52000, SDS-PAGE
Staphylococcus aureus
82000
-
gel filtration
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-tagatose 6-phosphate
Staphylococcus aureus
inducible by D-galactose
ADP + D-tagatose 1,6-bisphosphate
-
-
?
ATP + D-tagatose 6-phosphate
Staphylococcus aureus
involved in the catabolism of lactose and D-galactose
ADP + D-tagatose 1,6-bisphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Staphylococcus aureus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Staphylococcus aureus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
17.5
-
-
Staphylococcus aureus
Storage Stability
Storage Stability
Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 20% v/v glycerol, 0.2% v/v 2-mercaptoethanol, stable for several months
Staphylococcus aureus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose 6-phosphate
-
640993
Staphylococcus aureus
ADP + D-fructose 1,6-bisphosphate
-
-
-
-
ATP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
ADP + D-tagatose 1,6-bisphosphate
-
-
-
?
ATP + D-tagatose 6-phosphate
inducible by D-galactose
640993
Staphylococcus aureus
ADP + D-tagatose 1,6-bisphosphate
-
-
-
?
ATP + D-tagatose 6-phosphate
involved in the catabolism of lactose and D-galactose
640993
Staphylococcus aureus
ADP + D-tagatose 1,6-bisphosphate
-
-
-
?
CTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
CDP + D-tagatose 1,6-bisphosphate
-
-
-
?
GTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
GDP + D-tagatose 1,6-bisphosphate
-
-
-
?
ITP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
IDP + D-tagatose 1,6-bisphosphate
-
-
-
?
TTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
TDP + D-tagatose 1,6-bisphosphate
-
-
-
?
UTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
UDP + D-tagatose 1,6-bisphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
alpha2, 2 * 52000, SDS-PAGE
Staphylococcus aureus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
in potassium phosphate buffer
Staphylococcus aureus
8
-
in Hepes buffer
Staphylococcus aureus
8.5
-
in glycylglycine buffer, bicine buffer and Tris buffer
Staphylococcus aureus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
pH 7.5: about 75% of maximal activity, pH 9.5: about 55% of maximal activity, glycylglycine buffer
Staphylococcus aureus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NH4+
33.3 mM activates
Staphylococcus aureus
General Stability (protein specific)
General Stability
Organism
2-mercaptoethanol stabilizes during purification
Staphylococcus aureus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Li+
33.3 mM, 65% inhibition
Staphylococcus aureus
Na+
33.3 mM, 31% inhibition
Staphylococcus aureus
NEM
-
Staphylococcus aureus
phosphate
at high concentrations
Staphylococcus aureus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
D-tagatose 6-phosphate
-
Staphylococcus aureus
0.15
-
D-fructose 6-phosphate
-
Staphylococcus aureus
0.16
-
ATP
-
Staphylococcus aureus
0.17
-
ITP
-
Staphylococcus aureus
0.4
-
GTP
-
Staphylococcus aureus
0.9
-
UTP
-
Staphylococcus aureus
18
-
CTP
-
Staphylococcus aureus
20
-
TTP
-
Staphylococcus aureus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
8% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
Co2+
54% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
K+
33.3 mM activates
Staphylococcus aureus
Mg2+
absolute requirement for a divalent cation, a Mg2+ : ATP ratio of 2.0-2.5 is required for maximal activity
Staphylococcus aureus
Mn2+
27% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
Ni2+
15% of the activation with Mg2+, at 1.3 mM
Staphylococcus aureus
Rb+
33.3 mM activates
Staphylococcus aureus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
alpha2, 2 * 52000, SDS-PAGE
Staphylococcus aureus
82000
-
gel filtration
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-tagatose 6-phosphate
Staphylococcus aureus
inducible by D-galactose
ADP + D-tagatose 1,6-bisphosphate
-
-
?
ATP + D-tagatose 6-phosphate
Staphylococcus aureus
involved in the catabolism of lactose and D-galactose
ADP + D-tagatose 1,6-bisphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Staphylococcus aureus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
17.5
-
-
Staphylococcus aureus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 20% v/v glycerol, 0.2% v/v 2-mercaptoethanol, stable for several months
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose 6-phosphate
-
640993
Staphylococcus aureus
ADP + D-fructose 1,6-bisphosphate
-
-
-
-
ATP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
ADP + D-tagatose 1,6-bisphosphate
-
-
-
?
ATP + D-tagatose 6-phosphate
inducible by D-galactose
640993
Staphylococcus aureus
ADP + D-tagatose 1,6-bisphosphate
-
-
-
?
ATP + D-tagatose 6-phosphate
involved in the catabolism of lactose and D-galactose
640993
Staphylococcus aureus
ADP + D-tagatose 1,6-bisphosphate
-
-
-
?
CTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
CDP + D-tagatose 1,6-bisphosphate
-
-
-
?
GTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
GDP + D-tagatose 1,6-bisphosphate
-
-
-
?
ITP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
IDP + D-tagatose 1,6-bisphosphate
-
-
-
?
TTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
TDP + D-tagatose 1,6-bisphosphate
-
-
-
?
UTP + D-tagatose 6-phosphate
-
640993
Staphylococcus aureus
UDP + D-tagatose 1,6-bisphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha2, 2 * 52000, SDS-PAGE
Staphylococcus aureus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
in potassium phosphate buffer
Staphylococcus aureus
8
-
in Hepes buffer
Staphylococcus aureus
8.5
-
in glycylglycine buffer, bicine buffer and Tris buffer
Staphylococcus aureus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
pH 7.5: about 75% of maximal activity, pH 9.5: about 55% of maximal activity, glycylglycine buffer
Staphylococcus aureus
Other publictions for EC 2.7.1.144
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674827
Miallau
Structures of Staphylococcus a ...
Staphylococcus aureus
J. Biol. Chem.
282
19948-19957
2007
-
-
1
1
1
-
-
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640997
Jagusztyn-Krynicka
Streptococcus mutans serotype ...
Streptococcus mutans
J. Bacteriol.
174
6152-6158
1992
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5189
Van Rooijen
Molecular cloning, characteriz ...
Lactobacillus delbrueckii subsp. lactis
J. Biol. Chem.
266
7176-7181
1991
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640992
Anderson
-
D-Tagatose-6-phosphate kinase ...
Staphylococcus aureus
Methods Enzymol.
90
87-91
1982
1
-
-
-
-
-
2
6
-
2
-
1
-
1
-
-
1
-
-
1
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
6
-
2
-
1
-
-
-
1
-
1
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
640993
Bissett
Lactose and D-galactose metabo ...
Staphylococcus aureus
J. Biol. Chem.
255
8745-8749
1980
1
-
-
-
-
1
4
8
-
7
2
2
-
2
-
-
1
-
-
-
1
1
9
1
-
-
-
-
3
1
-
-
-
-
-
1
-
-
-
-
-
1
-
4
-
8
-
7
2
2
-
-
-
1
-
-
1
1
9
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
640995
Hamilton
Lactose metabolism by Streptoc ...
Streptococcus mutans
J. Bacteriol.
140
1102-1104
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
11
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640996
Schleifer
-
Occurence of D-tagatose-6-phos ...
no activity in Lactobacillus plantarum, no activity in Streptococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis
FEMS Microbiol. Lett.
3
9-11
1978
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-