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Information on EC 2.7.1.100 - S-methyl-5-thioribose kinase and Organism(s) Oryza sativa and UniProt Accession Q7XR61

for references in articles please use BRENDA:EC2.7.1.100
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IUBMB Comments
CTP also acts, but more slowly.
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This record set is specific for:
Oryza sativa
UNIPROT: Q7XR61
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Word Map
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
mtr kinase, 5-methylthioribose kinase, methylthioribose kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methylthioribose kinase
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MTR kinase1
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ATP:S-methylthioribose kinase
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kinase, 5-methylthioribose (phosphorylating)
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methylthioribose kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:S-methyl-5-thio-D-ribose 1-phosphotransferase
CTP also acts, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
68247-56-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + S-methyl-5-thio-D-ribose
ADP + S-methyl-5-thio-D-ribose 1-phosphate
show the reaction diagram
enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle
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-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0168
S-methyl-5-thio-D-ribose
pH 8.5, 37°C
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strong upregulation of enzyme expression during sulfur starvation
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involed in the methionine recycling
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48600
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE
50000
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, overvexpression of a slightly modified enzyme in Escherichia coli strain BL21 as GST-fusion protein
gene MTK1, quantitative realtime PCR enzyme expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
proteomic analysis of rice root proteins in untreated and in roots infected with Herbaspirillum seropedicae SmR1, overview. The 5-methylthioribose kinase is upregulated. quantitative realtime PCR expression analysis also confirms that the methionine recycling genes are upregulated in rice roots colonized by bacteria
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sauter, M.; Cornell, K.A.; Beszteri, S.; Rzewuski, G.
Functional analysis of methylthioribose kinase genes in plants
Plant Physiol.
136
4061-4071
2004
Arabidopsis thaliana (Q9C6D2), Oryza sativa (Q7XR61), Oryza sativa
Manually annotated by BRENDA team
Alberton, D.; Mueller-Santos, M.; Brusamarello-Santos, L.C.; Valdameri, G.; Cordeiro, F.A.; Yates, M.G.; de Oliveira Pedrosa, F.; de Souza, E.M.
Comparative proteomics analysis of the rice roots colonized by Herbaspirillum seropedicae strain SmR1 reveals induction of the methionine recycling in the plant host
J. Proteome Res.
12
4757-4768
2013
Oryza sativa (Q7XR61), Oryza sativa
Manually annotated by BRENDA team