The enzyme from parsley (Petroselinum crispum) is specific for umbelliferone and prenyl diphosphate. A minor product is osthenol, which is produced by transfer of the prenyl group to C-8 of umbelliferone.
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The enzyme appears in viruses and cellular organisms
The enzyme from parsley (Petroselinum crispum) is specific for umbelliferone and prenyl diphosphate. A minor product is osthenol, which is produced by transfer of the prenyl group to C-8 of umbelliferone.
enzyme has strict substrate specificity towards umbelliferone and dimethylallyl diphosphate, and a strong preference for the C6 position of the prenylated product (demethylsuberosin), leading to linear furanocoumarins. The C8-prenylated derivative (osthenol) is also formed, but to a much lesser extent
enzyme catalyzes the 6-prenylation of umbelliferone in the course of phytoalexin synthesis in Ammi majus cell suspension cultures in response to elicitor treatment
expression in the coumarin-producing plant Ruta graveolens shows increased consumption of endogenous umbelliferone. Expression of the prenyltransferase and a 4âcoumaroyl CoA 2'âhydroxylase gene in Nicotiana benthamiana, which does not produce furanocoumarins, results in formation of demethylsuberosin
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History
2.5.1.139 umbelliferone 6-dimethylallyltransferase
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