KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
umbelliferone | product demethylsuberosin, pH 8.0, 25°C | Petroselinum crispum | |
0.025 | - |
umbelliferone | product osthenol, pH 8.0, 25°C | Petroselinum crispum | |
0.045 | - |
dimethylallyl diphosphate | product osthenol, pH 8.0, 25°C | Petroselinum crispum | |
0.079 | - |
dimethylallyl diphosphate | product demethylsuberosin, pH 8.0, 25°C | Petroselinum crispum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Petroselinum crispum | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum crispum | W0SKS3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + umbelliferone | - |
Petroselinum crispum | diphosphate + demethylsuberosin | - |
? | |
dimethylallyl diphosphate + umbelliferone | - |
Petroselinum crispum | diphosphate + osthenol | minor product besides demethylsuberosin, about 5% of the amount of demethylsuberosin | ? | |
additional information | enzyme has strict substrate specificity towards umbelliferone and dimethylallyl diphosphate, and a strong preference for the C6 position of the prenylated product (demethylsuberosin), leading to linear furanocoumarins. The C8-prenylated derivative (osthenol) is also formed, but to a much lesser extent | Petroselinum crispum | ? | - |
? |
Organism | Comment | Expression |
---|---|---|
Petroselinum crispum | expression in various parsley tissues is increased by UV irradiation, with a concomitant increase in furanocoumarin production | up |
General Information | Comment | Organism |
---|---|---|
physiological function | expression in the coumarin-producing plant Ruta graveolens shows increased consumption of endogenous umbelliferone. Expression of the prenyltransferase and a 4coumaroyl CoA 2'hydroxylase gene in Nicotiana benthamiana, which does not produce furanocoumarins, results in formation of demethylsuberosin | Petroselinum crispum |