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Information on EC 2.5.1.138 - coumarin 8-geranyltransferase and Organism(s) Citrus limon and UniProt Accession A0A077K8G3

for references in articles please use BRENDA:EC2.5.1.138
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EC Tree
IUBMB Comments
The enzyme, characterized from the plant Citrus limon, is specific for geranyl diphosphate as a prenyl donor. It also has low activity with the coumarins 5,7-dihydroxycoumarin and 5-methoxy-7-hydroxycoumarin.
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This record set is specific for:
Citrus limon
UNIPROT: A0A077K8G3
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The taxonomic range for the selected organisms is: Citrus limon
The expected taxonomic range for this enzyme is: Citrus limon
Synonyms
clpt1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
umbelliferone 8-geranyltransferase
-
ClPT1
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate:umbelliferone 8-geranyltransferase
The enzyme, characterized from the plant Citrus limon, is specific for geranyl diphosphate as a prenyl donor. It also has low activity with the coumarins 5,7-dihydroxycoumarin and 5-methoxy-7-hydroxycoumarin.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + 5,7-dihydroxycoumarin
diphosphate + ?
show the reaction diagram
-
-
-
?
geranyl diphosphate + 5-methoxy-7-hydroxycoumarin
diphosphate + ?
show the reaction diagram
-
-
-
?
geranyl diphosphate + esculetin
diphosphate + 8-geranylesculetin
show the reaction diagram
-
-
-
?
geranyl diphosphate + umbelliferone
diphosphate + 8-geranylumbelliferone
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
assay in presence of Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
10 mM, complete inhibition
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression level in young and mature fruit
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
protein contains multiple transmembrane domains
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CGT1A_CITLI
407
8
45250
Swiss-Prot
other Location (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Munakata, R.; Inoue, T.; Koeduka, T.; Karamat, F.; Olry, A.; Sugiyama, A.; Takanashi, K.; Dugrand, A.; Froelicher, Y.; Tanaka, R.; Uto, Y.; Hori, H.; Azuma, J.; Hehn, A.; Bourgaud, F.; Yazaki, K.
Molecular cloning and characterization of a geranyl diphosphate-specific aromatic prenyltransferase from lemon
Plant Physiol.
166
80-90
2014
Citrus limon (A0A077K8G3), Citrus limon
Manually annotated by BRENDA team