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Information on EC 2.4.99.4 - beta-galactoside alpha-2,3-sialyltransferase
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2.4.99.4 beta-galactoside alpha-2,3-sialyltransferaseIUBMB Comments
The acceptor is Galbeta1,3GalNAc-R, where R is H, a threonine or serine residue in a glycoprotein, or a glycolipid. Lactose can also act as acceptor. May be identical with EC 2.4.99.2 monosialoganglioside sialyltransferase.
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Word Map
- 2.4.99.4
-
sialylation
- st6gal
- alpha-2,6-sialyltransferase
-
st6galnac
-
st8sia
-
sialylmotifs
-
cmp-n-acetylneuraminic
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2.4.99.1
- alpha-1,3-fucosyltransferase
- drug development
- synthesis
- medicine
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
2,3SiaT, alpha 2,3-ST 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2,3SiaT
alpha 2,3-ST
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-
-
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alpha2-->3 sialyltransferase
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-
-
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alpha2-3-sialyltransferase 1
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multifunctional enzyme which has alpha2-6-sialyltransferase, alpha2-3-sialidase, and alpha2-3-trans-sialidase activities in addition to its major alpha2-3-sialyltransferase activity
alpha3-SA-T
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-
-
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alpha3SA-T
beta-D-Gal-(1-3)-D-GalNAc alpha-(2-3)-sialyltransferase
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-
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beta-galactoside alpha-2,3-sialyltransferase
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CMP-N-acetylneuraminate:beta-D-galactoside alpha2-3 sialyltransferase
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-
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CMP-Neu5Ac:Glbeta1-3GalNAc alpha2,3-sialyltransferase
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-
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CMP-NeuAc:galactoside(alpha2-3)-sialyltransferase
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-
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CMP-SA:Galbeta3GalNAc-R alpha3-sialyltransferase
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-
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CMP-sialic acid:Galbeta1-3GalNAc-R alpha3-sialyltransferase
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-
-
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Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
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-
-
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Gal-NAc6S
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-
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Galbeta1,3GalNAc alpha2,3-sialyltransferase
galbeta1,3galNAcalpha2,3-sialyltransferase
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NeuAc alpha-2,3-sialyltransferase
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-
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sialyltransferase, cytidine monophosphoacetylneuraminate-beta-galactoside alpha2-->3-
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SIATFL
-
-
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ST3Gal I
ST3Gal III
ST3GalA.1
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-
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ST3GalIA
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-
-
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ST3O
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-
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alpha3SA-T
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-
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Galbeta1,3GalNAc alpha2,3-sialyltransferase
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-
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ST3Gal I
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycosyl group transfer
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-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:beta-D-galactoside alpha-(2->3)-N-acetylneuraminyl-transferase
The acceptor is Galbeta1,3GalNAc-R, where R is H, a threonine or serine residue in a glycoprotein, or a glycolipid. Lactose can also act as acceptor. May be identical with EC 2.4.99.2 monosialoganglioside sialyltransferase.
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CAS REGISTRY NUMBER
COMMENTARY
71124-51-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CMP + 3-O-[alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc]-L-tyrosyl-[protein]
CMP-N-acetyl-beta-neuraminate + 3-O-[beta-D-Gal-(1->3)-alpha-D-GalNAc]-L-tyrosyl-[protein]
the enzyme synthesizes CMP-NeuAc by transferring NeuAc from the alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-unit of O-glycans
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-
r
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
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-
-
?
CMP-N-acetyl-beta-neuraminate + benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
CMP + ?
-
-
-
?
CMP-N-acetyl-beta-neuraminate + benzyl beta-D-Gal-(1->3)-alpha-D-GalNAc
CMP + benzyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc
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-
-
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?
CMP-N-acetyl-beta-neuraminate + Galbeta(1->3)(GlcNAcbeta(1->6))GalNAc-OBn
CMP + ?
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-
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?
CMP-N-acetyl-beta-neuraminate + Galbeta(1->3)GalNAc-OBn
CMP + Neu5Acalpha(2->3)Galbeta(1->3)GalNAc-OBn
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-
-
?
CMP-N-acetyl-beta-neuraminate + octyl beta-D-Gal-(1->3)-alpha-D-GalNAc
CMP + octyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc
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-
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
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-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
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?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
-
?
CMP-N-acetyl-beta-neuraminate + [porcine submaxillary asialo-mucin]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [porcine submaxillary asialo-mucin]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
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-
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?
CMP-N-acetyl-beta-neuraminate + [porcine submaxillary asialo-mucin]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [porcine submaxillary asialo-mucin]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
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-
-
?
CMP-N-acetylneuraminate + 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
CMP + 4-methylumbelliferyl alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucoside
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best substrate, about 37% conversion
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-
?
CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
CMP + 4-nitrophenyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc
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-
-
-
?
CMP-N-acetylneuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
best acceptor substrate
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-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
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the enzyme is involved in O-linked glycosylation pathway for synthesis of core 1 and core 2 oligosaccharides
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?
CMP-N-acetylneuraminate + lactose
CMP + NeuAcalpha(2->3)Galbeta(1->4)Glc
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-
-
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?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
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-
-
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?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
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-
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?
CMP-N-acetyl-beta-neuraminate + lactose
CMP + alpha2,3-SiaLac + Neu5Ac
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-
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?
CMP-N-acetyl-beta-neuraminate + lactose
CMP + alpha2,3-SiaLac + Neu5Ac
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-
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?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
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-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
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-
?
additional information
?
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MeO-3-Galbeta(1->3)GalNAc-OBn is a poor acceptor for the enzyme
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additional information
?
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MeO-3-Galbeta(1->3)GalNAc-OBn is a poor acceptor for the enzyme
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additional information
?
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no activity with fetuin or alpha-1-acid glycoprotein (asialoorosomucoid)
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additional information
?
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with glycoprotein and ganglioside acceptors the substrate specificity is strict, with the sequence beta-D-Gal-(1->3)-alpha-D-GalNAc serving as the exclusive acceptor. The enzyme has an equilibrium random order mechanism
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
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-
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
-
-
-
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
-
-
?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
the enzyme is involved in O-linked glycosylation pathway for synthesis of core 1 and core 2 oligosaccharides
-
-
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
-
-
-
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Sodium citrate
direct sialylation using CMP-N-acetyl-beta-neuraminate
additional information
-
overexpression of N-acetylglucosaminyltransferase III significantly inhibits alpha2,3-sialylation
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
lysophosphatidylcholine
C16-18 acyl derivatives of lysophosphatidylcholine stimulate the activity of enzyme form A more extensively than the C14 acyl derivative, and the C12 acyl derivative is without effect on enzyme form A
phospholipid/octylglucoside liposomes
incorporation into phospholipid/octylglucoside liposomes activates enzyme form A
-
Triton X-100
stimulates activity of enzyme form A. Enzyme form B is fully active in absence of detergents. Detergents stimulate the activity of enzyme form A by lowering the KD and KM of CMP-N-acetylneuraminate and increasing the Vmax of the reaction
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma
Expression and function of alpha 2,3-sialyl- and alpha 1,3/1,4-fucosyltransferases in colon adenocarcinoma cell lines: role in synthesis of E-selectin counter-receptors.
beta-galactoside alpha-2,3-sialyltransferase deficiency
Sialyltransferase specificity in selectin ligand formation.
Brain Neoplasms
Effect of Golgi membrane phospholipid composition on the molecular species of GM3 gangliosides synthesized by rat liver sialyltransferase.
Breast Neoplasms
Multiplex reverse transcription polymerase chain reaction assessment of sialyltransferase expression in human breast cancer.
Breast Neoplasms
Prognostic value of tumoral sialyltransferase expression and circulating E-selectin concentrations in node-negative breast cancer patients.
Carcinoma
alpha-2,3-Sialyltransferase type 3N and alpha-1,3-fucosyltransferase type VII are related to sialyl Lewis(x) synthesis and patient survival from lung carcinoma.
Carcinoma
Altered mRNA expression of sialyltransferase in squamous cell carcinomas of the cervix.
Carcinoma
Expression of ST3Gal, ST6Gal, ST6GalNAc and ST8Sia in human hepatic carcinoma cell lines, HepG-2 and SMMC-7721 and normal hepatic cell line, L-02.
Carcinoma, Hepatocellular
Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver.
Carcinoma, Squamous Cell
Altered mRNA expression of sialyltransferase in squamous cell carcinomas of the cervix.
Carcinoma, Squamous Cell
Enhanced sialyltransferases transcription in cervical intraepithelial neoplasia.
Hypertension, Pulmonary
Multiplex reverse transcription polymerase chain reaction assessment of sialyltransferase expression in peripheral blood mononuclear cells in systemic sclerosis.
Infections
Alpha-2,3-sialyltransferase enhances Neisseria gonorrhoeae survival during experimental murine genital tract infection.
Leukemia
Processing O-glycan core 1, Gal beta 1-3GalNAc alpha-R. Specificities of core 2, UDP-GlcNAc: Gal beta 1-3 GalNAc-R(GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase and CMP-sialic acid: Gal beta 1-3GalNAc-R alpha 3-sialyltransferase.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Biosynthesis of O-glycans in leukocytes from normal donors and from patients with leukemia: increase in O-glycan core 2 UDP-GlcNAc:Gal beta 3 GalNAc alpha-R (GlcNAc to GalNAc) beta(1-6)-N-acetylglucosaminyltransferase in leukemic cells.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Processing O-glycan core 1, Gal beta 1-3GalNAc alpha-R. Specificities of core 2, UDP-GlcNAc: Gal beta 1-3 GalNAc-R(GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase and CMP-sialic acid: Gal beta 1-3GalNAc-R alpha 3-sialyltransferase.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Role of aberrant sialylation of chronic myeloid leukemia granulocytes on binding and signal transduction by chemotactic peptides and colony stimulating factors.
Leukemia, Myeloid, Acute
Effect of ST3GAL 4 and FUT 7 on sialyl Lewis X synthesis and multidrug resistance in human acute myeloid leukemia.
Leukemia, Myeloid, Acute
Processing O-glycan core 1, Gal beta 1-3GalNAc alpha-R. Specificities of core 2, UDP-GlcNAc: Gal beta 1-3 GalNAc-R(GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase and CMP-sialic acid: Gal beta 1-3GalNAc-R alpha 3-sialyltransferase.
Neoplasms
Alpha2,6-sialylation of cell-surface N-glycans inhibits glioma formation in vivo.
Neoplasms
Prognostic value of tumoral sialyltransferase expression and circulating E-selectin concentrations in node-negative breast cancer patients.
Neuroblastoma
Effect of Golgi membrane phospholipid composition on the molecular species of GM3 gangliosides synthesized by rat liver sialyltransferase.
Ovarian Neoplasms
Human beta-galactoside alpha-2,3-sialyltransferase (ST3Gal III) attenuated Taxol-induced apoptosis in ovarian cancer cells by downregulating caspase-8 activity.
Ovarian Neoplasms
Human GM3 Synthase Attenuates Taxol-Triggered Apoptosis Associated with Downregulation of Caspase-3 in Ovarian Cancer Cells.
Prostatic Neoplasms
Androgen-regulated transcriptional control of sialyltransferases in prostate cancer cells.
Reproductive Tract Infections
Alpha-2,3-sialyltransferase enhances Neisseria gonorrhoeae survival during experimental murine genital tract infection.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.02 - 1.388
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
-
0.04903
CMP-N-(4-pentynoyl)neuraminic acid
-
recombinant enzyme DELTA56ST3GalI, at pH 6.2 and 37°C
-
0.008
Galbeta(1->3)(GlcNAcbeta(1->6))GalNAc-OBn
isoform ST3Gal-I, at pH 6.0 and 25°C
0.35 - 0.39
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
0.54
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.67
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.71
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
1.4
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.02
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
-
0.026
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
wild type enzyme, at pH 7.4 and 37°C
-
0.096
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N170A, at pH 7.4 and 37°C
-
0.105
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme S148A, at pH 7.4 and 37°C
-
0.211
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N147S, at pH 7.4 and 37°C
-
0.352
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230F, at pH 7.4 and 37°C
-
0.444
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y191A, at pH 7.4 and 37°C
-
0.549
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315Y, at pH 7.4 and 37°C
-
0.854
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230A, at pH 7.4 and 37°C
-
1.388
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315A, at pH 7.4 and 37°C
-
0.04123
CMP-N-acetyl-beta-neuraminate
-
recombinant enzyme DELTA56ST3GalI, at pH 6.2 and 37°C
0.106
CMP-N-acetyl-beta-neuraminate
wild type enzyme, at pH 7.4 and 37°C
0.121
CMP-N-acetyl-beta-neuraminate
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
0.176
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230F, at pH 7.4 and 37°C
0.18
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315A, at pH 7.4 and 37°C
0.184
CMP-N-acetyl-beta-neuraminate
mutant enzyme N170A, at pH 7.4 and 37°C
0.205
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230A, at pH 7.4 and 37°C
0.271
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315Y, at pH 7.4 and 37°C
0.481
CMP-N-acetyl-beta-neuraminate
mutant enzyme S148A, at pH 7.4 and 37°C
0.668
CMP-N-acetyl-beta-neuraminate
mutant enzyme N147S, at pH 7.4 and 37°C
0.876
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y191A, at pH 7.4 and 37°C
0.18
CMP-N-acetylneuraminate
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.34
CMP-N-acetylneuraminate
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.44
CMP-N-acetylneuraminate
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.62
CMP-N-acetylneuraminate
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.35
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form A
-
0.39
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form B
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.2 - 3.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
-
2 - 8
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
17
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
19
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
47
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.2
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315Y, at pH 7.4 and 37°C
-
0.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y191A, at pH 7.4 and 37°C
-
0.65
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N170A, at pH 7.4 and 37°C
-
0.8
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N147S, at pH 7.4 and 37°C
-
2.28
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
-
2.32
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme S148A, at pH 7.4 and 37°C
-
2.42
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315A, at pH 7.4 and 37°C
-
2.97
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230A, at pH 7.4 and 37°C
-
3.15
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
wild type enzyme, at pH 7.4 and 37°C
-
3.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230F, at pH 7.4 and 37°C
-
0.13
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315Y, at pH 7.4 and 37°C
0.62
CMP-N-acetyl-beta-neuraminate
mutant enzyme N170A, at pH 7.4 and 37°C
0.63
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y191A, at pH 7.4 and 37°C
1.05
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315A, at pH 7.4 and 37°C
1.21
CMP-N-acetyl-beta-neuraminate
mutant enzyme N147S, at pH 7.4 and 37°C
2.25
CMP-N-acetyl-beta-neuraminate
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
2.5
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230A, at pH 7.4 and 37°C
2.7
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230F, at pH 7.4 and 37°C
3.65
CMP-N-acetyl-beta-neuraminate
mutant enzyme S148A, at pH 7.4 and 37°C
19
CMP-N-acetylneuraminate
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
23
CMP-N-acetylneuraminate
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
26
CMP-N-acetylneuraminate
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
32
CMP-N-acetylneuraminate
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.37 - 121.15
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
-
28
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
32
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
34
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
39
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.37
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315Y, at pH 7.4 and 37°C
-
1.05
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y191A, at pH 7.4 and 37°C
-
1.75
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315A, at pH 7.4 and 37°C
-
3.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230A, at pH 7.4 and 37°C
-
3.78
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N147S, at pH 7.4 and 37°C
-
6.77
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N170A, at pH 7.4 and 37°C
-
9.85
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230F, at pH 7.4 and 37°C
-
22.07
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme S148A, at pH 7.4 and 37°C
-
114.17
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
-
121.15
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
wild type enzyme, at pH 7.4 and 37°C
-
0.47
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315Y, at pH 7.4 and 37°C
0.72
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y191A, at pH 7.4 and 37°C
1.87
CMP-N-acetyl-beta-neuraminate
mutant enzyme N147S, at pH 7.4 and 37°C
3.35
CMP-N-acetyl-beta-neuraminate
mutant enzyme N170A, at pH 7.4 and 37°C
5.83
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315A, at pH 7.4 and 37°C
7.58
CMP-N-acetyl-beta-neuraminate
mutant enzyme S148A, at pH 7.4 and 37°C
12.2
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230A, at pH 7.4 and 37°C
15.33
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230F, at pH 7.4 and 37°C
18.6
CMP-N-acetyl-beta-neuraminate
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
30
CMP-N-acetylneuraminate
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
69
CMP-N-acetylneuraminate
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
73
CMP-N-acetylneuraminate
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
140
CMP-N-acetylneuraminate
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
T-lymphocytes, Epstein-Barr virus immortalized B-lymphocytes
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
physiological function
metabolism
the enzyme is involved in biosynthesis of O-linked oligosaccharide chains of glycoproteins
metabolism
the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
physiological function
-
ST3Gal III overexpression inhibits Taxol-triggered caspase-8 activation. ST3Gal III upregulation produces cellular resistance to taxol and hence reduces the efficacy of taxol therapy
physiological function
-
the enzyme modulates pancreatic cancer cell motility and adhesion in vitro and enhances its metastatic potential in vivo. Isoform ST3Gal III increases de novo expression of SLex by enzymatic competition, enhances pancreatic adenocarcinoma cells adhesion to rh-E-selectin, and increases tumour cell adhesion to HSE cells
physiological function
-
the enzyme regulates ovarian cancer cell migration and peritoneal dissemination via epidermal growth factor receptor signaling
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SIA4B_MOUSE
350
1
40130
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4B_PANTR
350
1
40074
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4B_RAT
350
1
40166
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4C_HUMAN
333
1
38045
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4C_MOUSE
333
1
38058
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4C_PANTR
328
1
37274
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4C_RAT
333
1
38043
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4A_CHICK
342
1
39540
Swiss-Prot
Secretory Pathway (Reliability: 3)
SIA4A_HUMAN
340
1
39075
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4A_MOUSE
337
1
39073
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4A_PANTR
340
1
39059
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4A_PIG
343
1
39769
Swiss-Prot
Secretory Pathway (Reliability: 1)
SIA4B_HUMAN
350
1
40173
Swiss-Prot
Secretory Pathway (Reliability: 1)
Q6H8N0_RAT
340
1
39349
TrEMBL
Secretory Pathway (Reliability: 1)
Q8NFD3_HUMAN
338
1
38431
TrEMBL
Secretory Pathway (Reliability: 1)
G7LI40_MEDTR
443
1
49676
TrEMBL
Secretory Pathway (Reliability: 2)
Q5ND67_ORYLA
334
1
38166
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6P9FQD0_SHEEP
333
1
38414
TrEMBL
Secretory Pathway (Reliability: 1)
A0A2G9GZX7_9LAMI
398
1
45383
TrEMBL
Secretory Pathway (Reliability: 4)
A0A0B2NY86_GLYSO
442
1
49741
TrEMBL
Secretory Pathway (Reliability: 1)
A0A2P6QDI0_ROSCH
430
0
49026
TrEMBL
other Location (Reliability: 4)
Q70D59_ONCMY
326
1
37384
TrEMBL
Secretory Pathway (Reliability: 2)
Q8N6A7_HUMAN
328
0
37360
TrEMBL
Secretory Pathway (Reliability: 2)
Q4JEZ1_MEDTR
421
1
48203
TrEMBL
other Location (Reliability: 3)
A0A2P6P4M4_ROSCH
437
1
49602
TrEMBL
Secretory Pathway (Reliability: 1)
A0A2G9HZV1_9LAMI
442
1
48886
TrEMBL
Secretory Pathway (Reliability: 2)
B9SCR7_RICCO
434
1
50338
TrEMBL
other Location (Reliability: 1)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C59S/C64S
the mutant shows reduced activity compared to the wild type enzyme
N147S
the mutant shows strongly reduced activity compared to the wild type enzyme
N170A
the mutant shows strongly reduced activity compared to the wild type enzyme
S148A
the mutant shows strongly reduced activity compared to the wild type enzyme
V315A
the mutant shows strongly reduced activity compared to the wild type enzyme
V315Y
the mutant shows strongly reduced activity compared to the wild type enzyme
Y191A
the mutant shows strongly reduced activity compared to the wild type enzyme
Y230A
the mutant shows strongly reduced activity compared to the wild type enzyme
Y230F
the mutant shows strongly reduced activity compared to the wild type enzyme
E271F
-
the mutation does not affect alpha2-3-sialyltransferase activity
E271F/R313Y
-
the mutations do not affect alpha2-3-sialyltransferase activity
R313Y
-
the mutation does not affect alpha2-3-sialyltransferase activity
A151D
the mutant shows significantly improved properties as compared to the native enzyme. The mutant displays overall best performance in the synthesis of the GM3 trisaccharide (alpha2,3-Neu5Ac-Lac) from lactose in a one-pot, two-enzyme cascade
L387A
the mutant shows significantly improved properties as compared to the native enzyme
S359T/S360T
the mutant shows significantly improved properties as compared to the native enzyme
A151D
-
the mutant shows significantly improved properties as compared to the native enzyme. The mutant displays overall best performance in the synthesis of the GM3 trisaccharide (alpha2,3-Neu5Ac-Lac) from lactose in a one-pot, two-enzyme cascade
-
L387A
-
the mutant shows significantly improved properties as compared to the native enzyme
-
S359T/S360T
-
the mutant shows significantly improved properties as compared to the native enzyme
-
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-Select nickel affinity column chromatography and Superdex S200 gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme DELTA56ST3Gal I lacking the first 56 amino acids is expressed in HEK-293 cells
-
full-length ST3Gal III DNA fragment subcloned into the pEF6/V5-His TOPO expression vector. SKOV-3 cells transfected with ST3Gal III-EF6
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
taxol treatment upregulates ST3Gal III expression and forces expression of ST3Gal III attenuated taxol induced apoptosis. Taxol induces the expression of ST3Gal III by a post-transcriptional mechanism in SKOV-3 ovarian cancer cells
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
-
ST3Gal III can serve as a therapeutic target of taxol therapy
medicine
-
the enzyme is a prognostic factor for migration and peritoneal dissemination of human ovarian cancer cells. High enzyme expression is associated with advanced stage epithelial ovarian cancer
synthesis
synthesis
-
chemoenzymatic synthesis of sialylated oligosaccharides for their evaluation in a polysialyltransferase assay
synthesis
the enzyme can be exploited for the enzymatic synthesis of diverse sialyl products
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, S.; Day, T.W.; Choi, M.R.; Safa, A.R.
Human beta-galactoside alpha-2,3-sialyltransferase (ST3Gal III) attenuated Taxol-induced apoptosis in ovarian cancer cells by downregulating caspase-8 activity
Mol. Cell. Biochem.
331
81-88
2009
Homo sapiens
brenda
Sugiarto, G.; Lau, K.; Li, Y.; Khedri, Z.; Yu, H.; Le, D.T.; Chen, X.
Decreasing the sialidase activity of multifunctional Pasteurella multocida alpha2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis
Mol. Biosyst.
7
3021-3027
2011
Pasteurella multocida
brenda
Perez-Garay, M.; Arteta, B.; Pages, L.; de Llorens, R.; de Bolos, C.; Vidal-Vanaclocha, F.; Peracaula, R.
alpha2,3-Sialyltransferase ST3Gal III modulates pancreatic cancer cell motility and adhesion in vitro and enhances its metastatic potential in vivo
PLoS ONE
5
e12524
2010
Homo sapiens
brenda
Lu, J.; Isaji, T.; Im, S.; Fukuda, T.; Kameyama, A.; Gu, J.
Expression of N-acetylglucosaminyltransferase III suppresses alpha2,3-sialylation, and its distinctive functions in cell migration are attributed to alpha2,6-sialylation levels
J. Biol. Chem.
291
5708-5720
2016
Homo sapiens
brenda
Gupta, R.; Matta, K.L.; Neelamegham, S.
A systematic analysis of acceptor specificity and reaction kinetics of five human alpha(2,3)sialyltransferases Product inhibition studies illustrate reaction mechanism for ST3Gal-I
Biochem. Biophys. Res. Commun.
469
606-612
2016
Homo sapiens (Q11201), Homo sapiens
brenda
Chandrasekaran, E.V.; Xue, J.; Xia, J.; Locke, R.D.; Matta, K.L.; Neelamegham, S.
Reversible sialylation synthesis of cytidine 5-monophospho-N-acetylneuraminic acid from cytidine 5-monophosphate with alpha2,3-sialyl O-glycan-, glycolipid-, and macromolecule-based donors yields diverse sialylated products
Biochemistry
47
320-330
2008
Rattus norvegicus (Q11205)
brenda
Shigeta, S.; Winter, H.C.; Goldstein, I.J.
alpha-(2->3)- and alpha-(2->6)-Sialyltransferase activities present in three variants of Ehrlich tumor cells identification of the products derived from N-acetyllactosamine and beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->O)-Bn
Carbohydr. Res.
264
111-121
1994
Mus musculus
brenda
Sadler, J.E.; Rearick, J.I.; Paulson, J.C.; Hill, R.L.
Purification to homogeneity of a beta-galactoside alpha2 leads to 3 sialyltransferase and partial purification of an alpha-N-acetylgalactosaminide alpha2 leads to 6 sialyltransferase from porcine submaxillary glands
J. Biol. Chem.
254
4434-4443
1979
Sus scrofa (Q02745)
brenda
Rearick, J.I.; Sadler, J.E.; Paulson, J.C.; Hill, R.L.
Enzymatic characterization of beta D-galactoside alpha2 leads to 3 sialyltransferase from porcine submaxillary gland
J. Biol. Chem.
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1979
Sus scrofa (Q02745)
brenda
Westcott, K.R.; Hill, R.L.
Reconstitution of a porcine submaxillary gland beta-D-galactoside alpha 2-3 sialyltransferase into liposomes
J. Biol. Chem.
260
13116-13121
1985
Sus scrofa (Q02745)
brenda
Higgins, E.A.; Siminovitch, K.A.; Zhuang, D.; Brockhausen, I.; Dennis, J.W.
Aberrant O-linked oligosaccharide biosynthesis in lymphocytes and platelets from patients with the Wiskott-Aldrich syndrome
J. Biol. Chem.
266
6280-6290
1991
Homo sapiens
brenda
Sengupta, P.; Misra, A.K.; Suzuki, M.; Fukuda, M.; Hindsgaul, O.
Chemoenzymatic synthesis of sialylated oligosaccharides for their evaluation in a polysialyltransferase assay
Tetrahedron Lett.
44
6037-6042
2003
Rattus norvegicus
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brenda
Fessner, W.D.; Mertsch, A.; He, N.; Yi, D.; Kickstein, M.
An alpha2,3-sialyltransferase from Photobacterium phosphoreum with broad substrate scope Controlling hydrolytic activity by directed evolution
Chem. Eur. J.
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11614-11624
2020
Photobacterium phosphoreum (A5LHX0), Photobacterium phosphoreum JT-ISH-467 (A5LHX0)
brenda
Noel, M.; Gilormini, P.A.; Cogez, V.; Yamakawa, N.; Vicogne, D.; Lion, C.; Biot, C.; Guerardel, Y.; Harduin-Lepers, A.
Probing the CMP-sialic acid donor specificity of two human beta-D-galactoside sialyltransferases (ST3Gal I and ST6gal I) selectively acting on O- and N-glycosylproteins
ChemBioChem
18
1251-1259
2017
Homo sapiens
brenda
Wen, K.; Sung, P.; Hsieh, S.; Chou, Y.; Lee, O.; Wu, C.; Wang, P.
alpha2,3-Sialyltransferase type I regulates migration and peritoneal dissemination of ovarian cancer cells
Oncotarget
8
29013-29027
2017
Homo sapiens
brenda
Ortiz-Soto, M.E.; Reising, S.; Schlosser, A.; Seibel, J.
Structural and functional role of disulphide bonds and substrate binding residues of the human beta-galactoside alpha-2,3-sialyltransferase 1 (hST3Gal1)
Sci. Rep.
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17993
2019
Homo sapiens (Q11201), Homo sapiens
brenda
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